Gingival Crevicular PMN is a marker for the Periodontal Disease

龈沟 PMN 是牙周病的标志物

基本信息

批准号：
11672090
负责人：
NUMABE Yukihiro
金额：
$ 2.24万
依托单位：
The Nippon Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

PMNs are associated with host defence mechanism and tissue destruction in periodontal tissue. Alterations of PMN functions such as phagocytosis, chemotaxis, oxidative burst, enzyme release and F-actin formation play a pivotal role in periodontal pathogenesis. Furthermore, one of the major end-products of PMN activity is the extracellular release of active proteases. PMNs contain large amounts of neutral serine protease such as elastase, cathepsin and collagenase, these enzymes can degrade many important proteins in the extracellular matrix of periodontal tissue. In recent years, it has been suggested that PMN apoptosis plays a role in the regulation of inflammation in periodontal tissue and control the host immune response. Our study focused on the following themes to investigate the possibility of GCF as a marker of periodontal disease. 1. Alteration of PMN functions after stimulation by lipopolysaccharide and other chemical mediator. 2. Alteration of PMN elastase and other enzyme activities in gingival crevicular fluid (GCF). 3. Status of PMN apoptosis expression in gingival crevice and the relationship between effects of various factors around these cells (e.g. fluid, cytokine) and apoptosis induction. We employ a flowcytometry, cytoenzymology and laser scanning microscopy technique to investigate these phenomena. In summary, these results indicate that alteration GCF component is significantly related to the change of PMN functions and progression of periodontitis. Examination of PMNs in GCF may be most important factor for detecting the periodontal disease progression.

PMN与牙周组织中的宿主防御机理和组织破坏有关。 PMN功能的改变，例如吞噬作用，趋化性，氧化爆发，酶释放和F-肌动蛋白形成在牙周发病机理中起关键作用。此外，PMN活性的主要最终产物之一是活性蛋白酶的细胞外释放。 PMN包含大量中性丝氨酸蛋白酶，例如弹性酶，组织蛋白酶和胶原酶，这些酶可以降解牙周组织外基质中许多重要的蛋白质。近年来，有人提出，PMN凋亡在牙周组织的炎症调节中起作用并控制宿主免疫反应。我们的研究重点是以下主题，以研究GCF作为牙周疾病标志的可能性。 1。脂多糖和其他化学介质刺激后PMN功能的改变。 2。在牙龈裂纹液（GCF）中改变PMN弹性酶和其他酶活性。 3。牙龈缝隙中PMN凋亡表达的状态以及这些细胞周围各种因素的影响（例如液体，细胞因子）和凋亡诱导之间的关系。我们采用流程术，细胞综合学和激光扫描显微镜技术来研究这些现象。总而言之，这些结果表明，改变GCF成分与PMN功能的变化和牙周炎的进展显着相关。检查GCF中PMN的检查可能是检测牙周疾病进展的最重要因素。