Study on Salivary PMNs as a marker for the Periodontal Disease.

唾液PMNs作为牙周病标志物的研究。

• 批准号: 14571990
• 负责人: NUMABE Yukihiro
• 金额: $ 1.92万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571990/
• 关键词: Neutrophils; Saliva; Periodontal tissue; Periodontal disease; Flow Cytometry; Cytometry

PMNs are associated with host defense mechanism and tissue destruction in periodontal tissue. Alterations of PMN functions such as phagocytosis, chemotaxis, oxidative burst, enzyme release and F-actin formation play a pivotal role in periodontal pathogenesis. Furthermore, one of the major end-products of PMN activity is the extracellular release of active proteases. PMNs contain large amounts of neutral serine protease such as elastase, cathepsin and collagenase, these enzymes can degrade many important proteins in the extracellular matrix of periodontal tissue. In recent years, it has been suggested that PMN apoptosis plays a role in the regulation of inflammation in periodontal tissue and control the host immune response. Our study focused on the following themes to investigate the possibility of Salivary PMNs (SPMNs) as a marker for periodontal disease. Alteration of SPMN phagocytic functions and enzyme activities in periodontal disease patients and Smokers. Status of PMN apoptosis expression in saliva and the relationship between effects of various factors around these cells (e.g. fluid, cytokine) and apoptosis induction. We employ a flowcytometry, cytoenzymology and laser scanning microscopy technique to investigate these phenomena. In summary, these results indicate that alteration of environment around PMNs in saliva is significantly related to the change of PMN functions and progression of periodontitis. Examination of PMNs in Saliva may be important factor for detecting the periodontal disease progression.

PMN与牙周组织中的宿主防御机理和组织破坏有关。 PMN功能的改变，例如吞噬作用，趋化性，氧化爆发，酶释放和F-肌动蛋白形成在牙周发病机理中起关键作用。此外，PMN活性的主要最终产物之一是活性蛋白酶的细胞外释放。 PMN包含大量中性丝氨酸蛋白酶，例如弹性酶，组织蛋白酶和胶原酶，这些酶可以降解牙周组织外基质中许多重要的蛋白质。近年来，有人提出，PMN凋亡在牙周组织的炎症调节中起作用并控制宿主免疫反应。我们的研究重点是以下主题，以研究唾液PMN（SPMN）作为牙周疾病标记的可能性。牙周疾病患者和吸烟者中SPMN吞噬功能和酶活性的改变。 PMN唾液中PMN凋亡表达的状态以及这些细胞周围各种因素的影响（例如液体，细胞因子）和凋亡诱导之间的关系。我们采用流程术，细胞综合学和激光扫描显微镜技术来研究这些现象。总而言之，这些结果表明，唾液中PMN周围环境的改变与PMN功能的变化和牙周炎的进展显着相关。对唾液中PMN的检查可能是检测牙周疾病进展的重要因素。

项目成果

Analysis of Saliva for Periodontal Diagnosis and Monitoring

唾液分析用于牙周诊断和监测

• 发表时间: 2004
• 期刊: Journal of Dental Research 84・Special Issue
• 作者: 笹本 宝;伊藤 弘;沼部幸博;鴨井久一;Numabe Y
• 通讯作者: Numabe Y

Numabe Y: "Alterations of Salivary PMN Functions after Cigarette Smoking"Journal of Dental Research. 82・Special Issue. 394 (2003)

Numabe Y：“吸烟后唾液中性粒细胞功能的改变”《牙科研究杂志》82·特刊394（2003）。

Suppression of Apoptosis Expression in Oral PMNs after Mouthwash with Acidic Electrolyzed Water

酸性电解水漱口后口腔中性粒细胞凋亡表达的抑制

• 发表时间: 2002
• 期刊: The Japanese Journal of Conservative Dentistry 45
• 作者: Masami Miura;Yukihiro Numabe;Yukino Kanesaka;Yuko Kubota;Takara Sasamoto;Kyuichi Kamoi
• 通讯作者: Kyuichi Kamoi

ニコチンとコチニンによる好中球遊走能の変化について,-NIH Imageによる解析-

尼古丁和可替宁引起的中性粒细胞迁移能力的变化 -使用 NIH Image 进行分析-

• 发表时间: 2004
• 期刊: 日本歯科保存学雑誌 47
• 作者: 三浦雅美;沼部幸博;鴨井久一
• 通讯作者: 鴨井久一

Alterations of Salivary PMN Functions after Cigarette Smoking

吸烟后唾液中性粒细胞功能的改变

• 发表时间: 2003
• 期刊: Journal of Dental Research 82(special issue)
• 作者: Yukihiro Numabe;Masami Miura;Hiroaki Osozawa;Kyuichi Kamoi
• 通讯作者: Kyuichi Kamoi