In vitro preservation of medicinal plants

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The present research project was successful in micropropagation protocol and medium term preservation method for in vitro shoot culture of Scopolia japonica Maxim. (Solanaceae) and Atractylodes ovata DC. (Compositae). In vitro grown shoots were successfully stored at 2℃ up to 2 years. Reculturing of the shoots in freshly prepared Linsmaier and Skoog medium supplemented with indole-3-acetic acid (1μM) and 6-benzyladenine (10μM) showed hundred percentage viability. Excised shoots were rooted in LS medium containing none of plant growth regulators or indole-3-acetic acid (1μM).Three- and four-year-old in vitro raised plants of S.japonica and the regenerated plants of A.lancea cultivated in the field for one or two years did not show any detectable variability in their morphological as well as biochemical characters, i.e. the composition and contents of tropane alkaloids in the S.japonica rhizome and the contents of atractylon, a sesquiterpene component of essential oil in the A.lancea rhizome, respectively, when compared with the each control set.Results reveals that non-frozen cold storage of in vitro shoot cultures of medicinal plants can be used as a germplasm preservation system for a medium-duration without deterioration of their biochemical as well as biological characteristics.