In vitro preservation of medicinal plants

本课题成功地研究了东莨菪离体芽培养的微繁方案和中期保存方法。（茄科）和苍术。(菊科)。试管苗在2℃条件下成功保存2年。在新配制的Linsmaier和Skoog培养基中添加吲哚-3-乙酸（1μM）和6-苄基腺嘌呤（10μM），再生芽的存活率为100%。在不含植物生长调节剂和吲哚-3-乙酸（1μM）的LS培养基中生根。离体培养3年、4年和田间栽培1年、2年的金针叶再生植株在形态和生化特征（金针叶根茎中tropane生物碱的组成和含量，金针叶根茎精油中倍半萜成分苍术的含量）方面均未表现出明显的差异。结果表明，药用植物离体芽培养物的非冷冻冷藏可作为种质资源的中期保存体系，且不影响其生化和生物学特性。

The present research project was successful in micropropagation protocol and medium term preservation method for in vitro shoot culture of Scopolia japonica Maxim. (Solanaceae) and Atractylodes ovata DC. (Compositae). In vitro grown shoots were successfully stored at 2℃ up to 2 years. Reculturing of the shoots in freshly prepared Linsmaier and Skoog medium supplemented with indole-3-acetic acid (1μM) and 6-benzyladenine (10μM) showed hundred percentage viability. Excised shoots were rooted in LS medium containing none of plant growth regulators or indole-3-acetic acid (1μM).Three- and four-year-old in vitro raised plants of S.japonica and the regenerated plants of A.lancea cultivated in the field for one or two years did not show any detectable variability in their morphological as well as biochemical characters, i.e. the composition and contents of tropane alkaloids in the S.japonica rhizome and the contents of atractylon, a sesquiterpene component of essential oil in the A.lancea rhizome, respectively, when compared with the each control set.Results reveals that non-frozen cold storage of in vitro shoot cultures of medicinal plants can be used as a germplasm preservation system for a medium-duration without deterioration of their biochemical as well as biological characteristics.