The establishment of the system that predicts the prognosis of acute leukemia patients on the basis of the biological properties of leukemic blast progenitors.

建立基于白血病原始细胞生物学特性预测急性白血病患者预后的系统。

• 批准号: 11672291
• 负责人: NARA Nobuo
• 金额: $ 2.5万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672291/
• 关键词: acute myelogenous leukemia; residual leukemic cells; IgH gene rearrangement; WT-1 gene; Notch protein; Jagged protein; 白血病幹細胞

Acute myelogenous leukemia(AML)is highly lethal malignant homopathy. To cure AML patients, malignant keukemic cells should be completely purged from the patients. Most of AML patients can achieve complete remission by intensive chemotherapy and/or by bone marrow transplantation. Some of them, however, suffer from the relapse of AML.The major issue in leukemia therapy is how to detect the residual leukemic cells in the patients in complete remission and how to eradicate such residual leukemic cells.In the present study, we have tried to detect suitable methods which can detect the residual leukemic cells. For the purpose we used nested-PCR method and amplified IgH gene from the plasm of the patients with B-cell leukemia and lymphoma. Some of the patients showed the rearrangement of IgH gene, although they were in complete remission. It was of interest that those patients suffered from the relapse of the diseases in several months.Further studies will be required to establish the method that can predict the prognosis in any leukemia patient.

急性髓系白血病(AML)是一种高度致命性的恶性同源性疾病。要治愈AML患者，必须彻底清除患者体内的恶性白血病细胞。大多数AML患者可通过强化化疗和/或骨髓移植获得完全缓解。白血病治疗的主要问题是如何检测完全缓解患者体内残留的白血病细胞，以及如何清除这些残留的白血病细胞。为此，我们采用巢式聚合酶链式反应的方法，从B细胞白血病和淋巴瘤患者的血浆中扩增出IgH基因。部分患者虽然完全缓解，但仍有IgH基因重排。有趣的是，这些患者的疾病在几个月内复发。还需要进一步的研究来建立能够预测任何白血病患者预后的方法。

项目成果

Shuji Tohda, Chizuko Sakashita, Tetsuya Fukuda, Naomi Murakami, Nobuo Nara: "Establishment of a double Philadelphia chromosome-positive acute lymphoblustic leukemia-derired cell line, TMD5"Leukemia Research. 23. 255-261 (1999)

Shuji Tohda、Chizuko Sakashita、Tetsuya Fukuda、Naomi Murakami、Nobuo Nara：“双费城染色体阳性急性淋巴细胞白血病衍生细胞系 TMD5 的建立”白血病研究。

Tohda S,Murakami N.Nara N.: "Polymerase chain reaction detection of rearranged immunoglobulin heavy chain DNA in plasma samples is useful in the diagnosis of B-cell lymphoma"Internaional Journal of Hematology. 72. 74-78 (2000)

Tohda S、Murakami N.Nara N.：“血浆样本中重排免疫球蛋白重链 DNA 的聚合酶链反应检测可用于 B 细胞淋巴瘤的诊断”国际血液学杂志。

Bai A, Kojima H, Hori M, Nara N, +50thers: "Priming with G-CSF effectively enhances low-dose Ara-C induced in vivo apoptosis in myeloid leukemia cells."Experimental Hematology. 27. 259-265 (1999)

Bai A、Kojima H、Hori M、Nara N、50thers：“G-CSF 引发可有效增强低剂量 Ara-C 诱导的骨髓性白血病细胞体内凋亡。”实验血液学。

Tohda S, Sakashita C, Fukuda T, Murakami N, Nara N: "Establishment of a double Philadelphia chromosome-positive acute lymphoblastic leukemia derived cell line, TMD5 : effects of cytokines and differentiation inducers on growth of the cells."Leukemia Resea

Tohda S、Sakashita C、Fukuda T、Murakami N、Nara N：“双费城染色体阳性急性淋巴细胞白血病衍生细胞系 TMD5 的建立：细胞因子和分化诱导剂对细胞生长的影响。”白血病研究

Tohda S, Murakami N, Nara N: "Polymerase chain reaction detection of rearranged immunoglobulin heavy chain DNA in plasma samples is useful in the diagnosis of B-cell lymphoma."International Journal of Hematology. 72. 74-78 (2000)

Tohda S、Murakami N、Nara N：“血浆样本中重排免疫球蛋白重链 DNA 的聚合酶链反应检测可用于 B 细胞淋巴瘤的诊断。”国际血液学杂志。