EFFECTS OF IMPACT-LOADING ON BONE MASS AND STRENGTH IN OVARIECTOMIZED RATS

冲击载荷对去势大鼠骨质量和强度的影响

• 批准号: 11680062
• 负责人: UMEMURA Yoshihisa
• 金额: $ 0.58万
• 依托单位: CHUKYO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680062/
• 关键词: BONE MASS; BONE STRENGTH; MECHANICAL STRESS; JUMP EXERCISE; OVARIECTOMY; OSTEOCALCIN; ICTP; EXERCISE FREQUENCY; 骨重

The first study was designed to investigate the effect of high-impact and low-repetition jump exercise on bones in ovariectomized rats. Forty female Wistar rats were sham-operated (Sham) or ovariectomized (OVX) at the age of 11 weeks. The rats were randomly divided into the following 4 groups ; Sham-Sedentary (SS, n=10), Sham-Exercised (SE, n=10), OVX-Sedentary (OS, n=10), and OVX-Exercised (OE, n=10). The rats started the jump-training at the age of 12 weeks. The jump-training protocol was 10 times/day, 5 days/week and the jumping height was 40 cm. After 8 weeks of training, the mass and breaking force in the tibia and ulna, cross-sectional areas of diaphysis in the tibia and serum bone turnover markers were measured. The jump-training significantly increased the fat-free dry weight, ash weight and ultimate breaking force in the tibia. The rate of increase in these parameters was similar in both the Sham and OVX groups. On the other hand, in the ulna, there were no significant changes in the ultimate breaking force. The jump-training significantly increased the periosteal perimeter and cortical area, though the increase in these parameters in OE compared to OS was lower than that in SE compared to SS.The jump-training significantly increased serum osteocalcin in the OVX groups, as well as in the Sham groups.The second study was focused on exercise frequency in one week to increase bone mass and strength in ovariectomized rats. The experimental protocol was like as the first study. Jump exercise with only one times/week increased bone mass and strength in ovariectomized rats, though did not increase cross-sectional cortical area of diaphysis. These results suggest that high-impact and low-repetition training had beneficial effects on bone formation and bone biomechanical properties in OVX rats, as well as in Sham rats.

第一项研究旨在研究高冲击低重复跳跃运动对去卵巢大鼠骨骼的影响。雌性Wistar大鼠40只，于11周龄时行假手术(Sham)或去卵巢(OVX)。将大鼠随机分为4组：假安静组(SS，n=10)、假运动组(SE，n=10)、去卵巢运动组(OS，n=10)和去卵巢运动组(OE，n=10)。大鼠从12周龄开始跳跃训练。跳跃训练方案为10次/d，5天/周，跳跃高度为40 cm。训练8周后，测量胫骨和尺骨的质量、折断力、胫骨骨干横截面积和血清骨转换指标。跳跃训练显著提高了胫骨的脱脂干重、灰重和极限折断力。Sham组和OVX组这些参数的增加速度相似。而尺骨的极限折断力无明显变化。跳跃训练显著增加骨膜周长和皮质面积，但OE组的骨膜周长和皮质面积的增加低于SE组。跳跃训练显著升高OVX组和Sham组的血清骨钙素。第二项研究集中在一周内的运动频率以增加去卵巢大鼠的骨量和力量。实验方案与第一项研究相同。每周一次的跳跃运动增加了去卵巢大鼠的骨量和力量，但并没有增加骨干的横截面积。这些结果表明，高冲击低重复训练对OVX大鼠和Sham大鼠骨形成和骨生物力学性能均有良好的影响。

项目成果

梅村義久: "1回および2回のジャンプトレーニングがラットの骨量・強度に及ぼす影響."中京大学体育研究所紀要. 15. 47-49 (2001)

Yoshihisa Umemura：“一次和两次跳跃训练对大鼠骨量和力量的影响”，中京大学体育研究所公告 15. 47-49 (2001)。

長澤省吾: "ラットのジャンプトレーニングにおけるジャンプ時の床反力と骨強度および骨重量との関係"中京大学体育学論叢. 42(印刷中). (2001)

长泽正吾：“大鼠跳跃训练时地面反作用力与骨强度和骨重量的关系”中京大学体育丛书42（出版中）。

Y Kodama, Y Umemura, S Nagasawa, WG Beamer, LR Donahue, CR Rosen, DJ Baylink, JR Farley: "Exercise and Mechanical Loading Increase Periosteal Bone Formation and Whole Bone Strength in C57BL/6J Mice but Not in C3H/Hej Mice."Calcif Tissue Int. 66. 298-306 (

Y Kodama、Y Umemura、S Nagasawa、WG Beamer、LR Donahue、CR Rosen、DJ Baylink、JR Farley：“运动和机械负荷会增加 C57BL/6J 小鼠的骨膜骨形成和整个骨骼强度，但不会增加 C3H/Hej 小鼠的骨膜骨形成和整体骨骼强度。

梅村義久: "骨重量・骨強度を高めるジャンプトレーニング"バイオメカニクス研究. 3. 160-162 (1999)

Yoshihisa Umemura：“增加骨骼重量和力量的跳跃训练”生物力学研究 3. 160-162 (1999)。

A Honda, Y Umemura, S Nagasawa: "Effect of High-impact and Low-repetition Training on Bones in Ovariectomized Rats."Journal of Bone and Mineral Research. 16 (in press).. (2001)

A Honda、Y Umemura、S Nagasawa：“高冲击力和低重复训练对卵巢切除大鼠骨骼的影响”。《骨骼与矿物质研究杂志》。