Mechanism of dye decoloration using enzyme system bleaching agent for the purpose of environmental loading reduction-Reaction selectivity of dye-

以降低环境负荷为目的，使用酶系漂白剂进行染料脱色的机理-染料的反应选择性-

• 批准号: 11680099
• 负责人: MORITA Miyuki
• 金额: $ 2.37万
• 依托单位: Hokkaido University of Education
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680099/
• 关键词: enzyme system bleaching agent; dye decoloration; reaction selectivity

Using 3 kinds of pigments, the reaction which used the dye by the mixed system was examined. The decoloration rate constant increases more, as the oxidation-reduction potential is smaller. It is considered that the reason why the rate constant differs is because the dye space structure differs, though oxidation-reduction potential is equal. In the dye mixed system, it was not effective for the reaction between Orange I and HRP, even if Orange II and Orange G coexist, and the reaction of Orange I rapidly progressed. In the meantime, Orange II slowed down, and Orange G quickened. It seems to be a cause that the radical dye generation rate differs on this by the difference between enzymatic substrate specificity and dye oxidation-reduction potential. Therefore, the reaction mechanism of anticipated dye mixed system consists like the following. In case of the dye mixed system, since Orange I of the reaction is very rapider than that of other dye, the radical is formed from the right after reaction, and it decomposes afterwards. In the meantime, the reaction of Orange II does not start, since the Orange II oxidation-reduction potential is high, while Orange I is reacting with the enzyme prior. The reaction of Orange II starts after the reaction end of Orange I.It is also similar that reaction start of Orange G is late. It is based that the reaction rate of Orange II is also slower than the single system by mixed system on following reasons. In doing the role of the activator of Orange G, the orange II radical receives the electron in Orange G.The orange II radical returns in Orange II.It is considered that in the dye mixed system, the reaction of Orange II is suppressed further than the Orange II single system.

用3种颜料考察了混合体系中染料的反应。氧化还原电位越小，脱色速率常数越大。据认为，速率常数不同的原因是因为染料的空间结构不同，虽然氧化还原电位相同。在染料混合体系中，即使橙子II和橙子G共存，橙子I和HRP之间的反应也是无效的，并且橙子I的反应迅速进行。与此同时，橙子II减速，橙子G加速。这似乎是一个原因，自由基染料的产生速率不同，在这方面的酶底物特异性和染料氧化还原电位之间的差异。因此，预期的染料混合体系的反应机理如下。在染料混合体系的情况下，由于反应的橙子I比其它染料的反应快得多，因此在反应后立即形成自由基，然后它分解。同时，橙子II的反应不开始，因为橙子II的氧化-还原电位高，而橙子I先与酶反应。橙子II的反应在橙子I的反应结束之后开始。同样，橙子G的反应开始较晚。这是基于以下原因，橙子II的反应速率也比单一系统的混合系统慢。在起橙子G的活化剂作用时，橙子II自由基在橙子G中接收电子。橙子II自由基在橙子II中返回。认为在染料混合体系中，橙子II的反应比橙子II单一体系受到进一步抑制。