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Enzymatic Treatment of Textile Dysing Effluents

纺织印染废水的酶法处理

基本信息

批准号：
11680118
负责人：
KATAYAMA Michiko
金额：
$ 2.37万
依托单位：
Tokyo Kasei University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

The biodegradability of various kinds of dyes and their effluent treatment by use of an enzyme, Bilirubin oxidase (BO) are reported. Biodegradation of copper phthalocyanine (CP) was first examined by using the soil perfusion method. An aqueous CP solution was circulated through columns containing native and sterilized field soils, respectively. A rapid decrease to almost zero in both O.D.(λ max=622 nm) and total copper concentration in the solution was observed within 2 weeks in the native soil column, but no significant decrease in the sterilized soil column. A fungus growing on an agar medium containing 0.1% CP as a sole carbon source was isolated from the former column after 292 days of circulation. The fungus was identified as Myrothecium verrucaria according to the characteristic morphology of its phyalides. According to Murao and Tanaka (Agri.Biol.Chem., 1981, 45, 2383), Myrothecium verrucaria isolated from soil generates BO successfully. CP was efficiently decolorized by the BO under the optimum conditions of pH 5-7 and of temperatures 32-37℃. Aqueous solutions of 21 dyes (C.I.Acid Blue 25, 40, 41, 45, 80, 138, Acid Green 25, 27, Reactive Blue 2, 4, 5, 19, Reactive Black 5, Direct Blue 6, 15, 106, Direct Green 59, Direct Black 22, 51, Direct Violet 48, Direct Red 2) were decolorized by BO.The absorption spectrum of decolorized solutions were analysed by HPLC, and their intermediats were detected by the photodiode array analysis.

本文报道了各种染料的生物降解性及其用胆红素氧化酶（BO）处理染料废水的研究结果。首次采用土壤灌流法研究了酞菁铜（CP）的生物降解。CP水溶液分别通过含有天然和灭菌田间土壤的柱循环。两个O.D.都迅速下降到几乎为零。（λ max=622 nm）和总铜浓度在自然土柱中均在2周内出现下降，而在灭菌土柱中无明显下降。在循环292天后，从前一个柱中分离出在含有0.1%CP作为唯一碳源的琼脂培养基上生长的真菌。根据菌丝体的形态特征，鉴定该真菌为疣状漆斑菌（Myrothecium verrucaria）。根据Murao和Tanaka（Agri.Biol.Chem.，1981，45，2383），从土壤中分离的疣状漆斑菌（Myrothecium verrucaria）成功产生BO。BO对CP的最佳脱色条件为pH 5-7，温度32-37℃。21种染料的水溶液（C.I.酸性蓝25、40、41、45、80、138，酸性绿色25、27，活性蓝2、4、5、19，活性黑5，直接蓝6、15、106，直接绿色59，直接黑22、51，直接紫48，直接红2）用BO进行脱色，用高效液相色谱法分析脱色液的吸收光谱，用光电二极管阵列分析法检测脱色中间体。