Decomposition of dyes by Bilirubin Oxidase

为了考察酶对印染废水的脱色效果，用胆红素氧化酶（BO）对10种工业蒽醌染料和8种工业偶氮染料进行了脱色处理。通过测定加入该酶的染料溶液的可见光和紫外吸收光谱，证实了该酶对所有蒽醌染料的有效脱色。加入酶的纯化C.I.酸蓝40水溶液在40℃下摇匀。去除酶后，用高效液相色谱法分析规定时间后的等分溶液。色谱图显示，加入酶后染料（C.I. Acid Blue 40）立即开始分解。在停留时间5.2 min左右出现一个与分解络合物有关的峰值，加入酶后逐渐升高，约3 h后达到峰值，24 h后基本保持，但逐渐降低。产物在该峰处的吸收光谱由光电二极管阵列分析确定。通过测定该酶存在下染料溶液的可见光和tJV吸收光谱，证实了6种偶氮染料的有效脱色，但两种偶氮染料C.I.直接黑22和C.I.直接紫48的脱色效率不高。采用高效液相色谱法测定染料的脱色效果。色谱图显示，加入酶后，8种染料立即开始分解。根据色谱峰面积计算的脱色率，前六种染料约为100%，后两种染料约为90%。

In order to examine the decolonzation of dyeing effluent by enzyme, aqueous solutions of ten commercial anthraquinone dyes and eight commercial azo dyes were treated with Bilirubin oxidase (BO). Efficient decolorization ofall anthraquinone dyes was confirmed by measuring the visible and UV absorption spectra of the dye solution to which the enzyme was added.An aqueous solution of purified C.I. Acid Blue 40, to ^hich the enzyme wasadded, was shaken at 40 ℃.Aliquots of the solution after the specified time were analyzed by HPLC after removing the enzyme. Thechromatograms showed that the decomposition of dye (C.I. Acid Blue 40) started immediately after the addition of the enzyme. A peak around a retention time of 5.2 min,which can be related to the decomposition complex, increased gradually after the addition of the enzyme and was reached after about 3 h. That peak was nearly maintained even after 24 h, although it gradually decreased. Theabsorption spectrum of the product at that peak was determined by a photodiode array analysis.Efficient decolorization of six azo dyes was confirmed by measureing the visible and tJV absorption spectraof the dye solution in the presence of the enzyme, but that of the two azo dyes, C.I. Direct Black 22 and C.I.Direct Violet 48 was not efficient. Also HPLC was introduced to measure decolorization of dyes. The chromatograms showed that the decomposition of the eight dyes started immediately after the addition of the enzyme. The decolorization ratios calculated from the peak areas in the chromatograms were ca. 100% for first the six dyes and 90% for that last two dyes.