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The development of materials for foods and drugs by bio-power of marine microorganisms

利用海洋微生物的生物动力开发食品和药品材料

基本信息

批准号：
11794013
负责人：
KODAMA Seiji
金额：
$ 8.32万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for University and Society Collaboration
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11794013/
关键词：
marine microorganism library inhibition of melanogenesis inhibition of tumor cell growth red tide phytoplankton active oxygens mannuronate inhibition of cell motility endocrine desruptors 細胞増殖阻害ゲノムDNAライブラリー微生物ライブラリーグルロン酸アルギン酸

项目摘要

We constructed a marine microorganism library to find the bacteria that produce materials useful for developing new foods or drugs. This microorganism library provides us the following new results. First, we found two strains of marine bacteria showing the ability to inhibit melanogenesis that is comparable to that of 0. 25 mM ascorbic acid by screening 548 strains of marine bacteria. The bacteria-secreted inhibitors are heat-resistant and show no reductive activity. Then, we also found 24 strains of marine bacteria showing the ability to inhibit the growth of colon cancer cells by screening 2,480 strains of marine bacteria. In addition, we found that mannuronate polymer, one of components of alginate, suppressed the motility of glioblastoma cells having wild-type p53 gene after X-irradiation. Furthermore, we found that galacturonic acid stimulated Chattonella marina and Heterosigna akashiwo to generate increased amounts of O_2-, suggesting that the binding of galacturonic acid to specific sites on the flagellate cell surface may induce the increase of O_2- production. Finally, we established in vitro and in vivo screening systems that were hypersensitive to detect endocrine desruptors in the marine resources.

我们构建了一个海洋微生物库，以寻找产生可用于开发新食品或药物的材料的细菌。该微生物文库为我们提供了以下新结果。首先，我们发现了两种海洋细菌菌株，其抑制黑素生成的能力与0。通过筛选548株海洋细菌，获得25 mM抗坏血酸。细菌分泌的抑制剂是耐热的，并且不显示还原活性。然后，我们还通过筛选2，480株海洋细菌，发现了24株海洋细菌显示出抑制结肠癌细胞生长的能力。此外，我们发现，甘露糖醛酸聚合物，藻酸盐的成分之一，抑制了具有野生型p53基因的胶质母细胞瘤细胞在X射线照射后的运动。此外，我们还发现半乳糖醛酸刺激Chattonella marina和Heterosigna akashiwo产生大量的O_2-，这表明半乳糖醛酸与鞭毛细胞表面特定位点的结合可能诱导O_2-产生的增加。最后，我们建立了在体外和体内筛选系统，是高灵敏度的检测内分泌破坏剂的海洋资源。