Establishment of an assay system for biological effect of radiation using neural stem cells
Establishment of an assay system for biological effect of radiation using neural stem cells
批准号:
22651019
负责人:
KODAMA Seiji
金额:
$2.23万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011
中文摘要
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英文摘要
To establish an assay system for biological effect of radiation using neural stem cells, we used mouse neurosphere cells that contain neural stem cells, and fibroblast cells as a control. Neurosphere cells showed faster repair kinetics for DNA double strand breaks than fibroblast cells. To establish immortal neurosphere cell lines, we subcultured the neurosphere cells by successive transfer until the cells exceeded 100 population doubling numbers. Finally, we immortalized several mouse neurosphere cell lines that retained diploid karyotype and characteristics of neural stem cells.
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放射線被ばく染色体を介した染色体不安定性
由辐射暴露染色体介导的染色体不稳定性
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[児玉靖司, 白石一乗, 押村光雄]
通讯作者:
押村光雄
放射線被ばくした染色体を介した染色体不安定性
由辐射暴露染色体介导的染色体不稳定性
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[児玉靖司, 白石一乗, 押村光雄]
通讯作者:
押村光雄
マウスニューロスフェア形成細胞を用いた細胞不死化の試み
尝试使用小鼠神经球形成细胞进行细胞永生化
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[今西香絵, 白石一乗, 原正之, 児玉靖司]
通讯作者:
児玉靖司
UVA 照射染色体の移入による染色体不安定性の誘発
UVA 照射的染色体转移诱导染色体不稳定
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[漆原あゆみ, 児玉靖司]
通讯作者:
児玉靖司
染色体不安定性誘発における紫外線の影響
紫外线辐射对诱导染色体不稳定的影响
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[橋本翔子, 岡田和嗣, 今岡進, 漆原あゆみ]
通讯作者:
漆原あゆみ
共 24 条
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