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Development of Biosensor-type-DNA Chip for the Diagnosis of Cancer

开发用于癌症诊断的生物传感器型DNA芯片

基本信息

批准号：
11794017
负责人：
KANEKO Shuichi
金额：
$ 9.6万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for University and Society Collaboration
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11794017/
关键词：
Biosensor Cancer DNA chip SAGE Molecular Diagnosis 遺伝子

项目摘要

Development of a biosensor-type-DNA chip for the diagnosis of cancer is an objective of this research. To attain this objective, two lines of research projects were designed.For the first line of the project, we systematically examined expression profiles in the liver with various diseases including hepatocellular carcinoma. Over one hundred clinical materials were analyzed with home-made DNA chips, and the expression profile in each disease was demonstrated. This study revealed that a DNA chip analysis gives new information that cannot be obtained by routine biochemical and pathological examinations, and made the DNA chip be a new diagnostic technique, or a new tumor marker. Using serial analysis of gene expression (SAGE), over 300,000 expressed tags in the liver were obtained and deposited into databases. A DNA chip, which predicts the efficacy of drugs against chronic hepatitis C, was put on the market.Another line of the project is to make a biosensor-type-DNA chip, which can be used in routine clinical settings on site. Using microelectrodes on a chip, target nucleic acids can be measured in liquid (applied for a patent in 2001). A small-size thermal cycler for the introduction of measurement on microelectrodes was developed (applied for a patent in 2000). A DNA spotter on a microelectrode was also developed (pending for a patent). Using these successive developments, a biosensor-type-DNA chip, which is small-size, less expensive compared with former laser-detection-type DNA chips, and faster for the clinical diagnosis, was made.

本研究的目的之一是开发一种用于癌症诊断的生物传感器型DNA芯片。为了实现这一目标，我们设计了两条研究路线，第一条路线是系统地检测包括肝细胞癌在内的各种疾病肝脏中的表达谱。用自制的DNA芯片分析了100多份临床资料，并展示了每种疾病的表达谱。本研究揭示了DNA芯片分析提供了常规生化和病理检查无法获得的新信息，使DNA芯片成为一种新的诊断技术，或一种新的肿瘤标志物。使用基因表达系列分析（SAGE），获得了肝脏中超过300，000个表达的标签并存入数据库。该项目的另一条线是制作生物传感器型DNA芯片，可用于现场常规临床设置。使用芯片上的微电极，可以在液体中测量目标核酸（2001年申请专利）。开发了用于引入微电极测量的小型热循环仪（2000年申请专利）。还开发了微电极上的DNA点样器（正在申请专利）。利用这些连续的发展，生物传感器型DNA芯片，这是小尺寸，比以前的激光检测型DNA芯片更便宜，更快的临床诊断，被制成。