Effects of Magnetic field on Nerve Transmission and Analyses of Efficiency and Safety for Human on Application of Magnetic Field.

磁场对神经传递的影响及人体应用磁场的效率和安全性分析。

• 批准号: 11834009
• 负责人: IKEHARA Toshitaka
• 金额: $ 2.3万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11834009/
• 关键词: time-varying strong magnetic field; extremely-low-frequency magnetic field; intracellular Ca^<2+>; Ca^<2+>-dependent K^+ channel; endoplasmic reticulum; infrared spectroscopy; chromaffin cells; PC12 cells; イノシトール3燐酸; 誘導電流; ブラディキニン; アセチルコリン; 変動

1. The time-varying magnetic fields (MF) partly suppressed K^+ uptake through Ca^<2+>-dependent K^+ channels. When the cells were placed in the high K^+ medium, [Ca^<2+>]c increased to about 1.4 times the original level, but exposure to the magnetic fields completely suppressed the increase. Addition of ionomycin to the high K^+ medium increased [Ca^<2+>]c to the level of control cells, regardless of exposure to the magnetic field. But the inhibition of K^+ uptake by the magnetic field was not restored by addition of ionomycin. The suppression of K^+ influx could relate to a change in electric properties of cell surface.2. The time-varying MF inhibited the transient increase in [Ca^<2+>]c stimulated by acetylcholine, bradykinin, caffeine, ionomycin and thapsigargin in chromaffin cells. Also, [Ca^<2+>]c was markedly increased in inositol 1,4,5-trisphosphate-loaded cells, and this increase was inhibited by exposure. Inhibition of bradykinin-induced increase in [Ca^<2+>]c by exposure for … More 0.5 hr was mostly recovered after placing the cells for 1 hr outside the magnetic field. Our results indicate that the MF-exposure inhibits Ca^<2+> release from intracellular Ca^<2+> stores, but that bradykinin binding to bradykinin receptors of the cell membrane and inositol 1,4,5-trisphosphate production are not influenced.3. The effects of exposure to 50 Hz extremely-low-frequency (ELF) MF (maximally 41.7-43.6 mT) on structures of membrane protein of living HeLa cells were studied by the attenuated total reflection infrared spectroscopy. The exposure for 1 min shifted the peak absorbance of amide I band to a smaller wave number, strongly reduced the absorbance of amide II band and increased the absorbance at around 1600 cm^<-1>. These results suggest that exposure to the ELF magnetic field reversibly influences N-H inplane bendings and C-N stretching vibrations of peptide linkages, and changes secondary structures of alpha-helix and beta-sheet in proteins of the cell membrane.4. Exposure to ELF MF to 60 Hz MF (maximally 3 mT) further increased the differentiation of PC12 cells stimulated by addition of nerve growth factor. Less

1.时变磁场部分抑制了依赖于Ca~(2+)和Gt~(2+)的K~(++)通道对K~+的吸收。当细胞置于高K^+培养液中时，[Ca^&lt；2+&gt；]c增加到原来的1.4倍左右，但暴露在磁场中完全抑制了这种增加。在高K^+培养液中加入离子霉素使[Ca^&lt；2+&gt；]c增加到对照细胞的水平，与磁场暴露无关。但外加离子霉素不能恢复磁场对K+吸收的抑制作用。K~+内流的抑制可能与细胞表面电学性质的改变有关。时变MF抑制乙酰胆碱、缓激肽、咖啡因、离子霉素和thapsigargin刺激的嗜铬细胞[Ca~(2+)]_c的一过性升高。此外，在1，4，5-三磷酸肌醇负载的细胞中，[Ca~(2+)&t；2+&gt；]c显著升高，这种升高可被暴露所抑制。…抑制缓激肽诱导的[Ca~(2+)]_c升高在磁场外放置1小时后，细胞大部分恢复到0.5小时以上。我们的结果表明，磁场暴露抑制了细胞内钙离子的释放，但不影响缓激肽与细胞膜上缓激肽受体的结合以及1，4，5-三磷酸肌醇的产生。用衰减全反射红外光谱仪研究了50赫兹极低频(ELF)磁场(最大41.7-43.6mT)对活体HeLa细胞膜蛋白结构的影响。暴露1min使酰胺I带的峰值吸光度移动到一个较小的波数，显著降低了酰胺II带的吸光度，并在1600 cm~(-1)~(-1)处增大了吸光度。这些结果表明，暴露在ELF磁场中可逆地影响了多肽键的N-H面内弯曲和C-N伸缩振动，并改变了细胞膜蛋白质中α-螺旋和β-折叠的二级结构。暴露于60赫兹磁场(最大3mT)的极低频磁场进一步增加了由神经生长因子刺激的PC12细胞的分化。较少

项目成果

福井康広、池原敏孝他: "ELF磁界による歯根膜誘導電流の解析ー歯槽骨再生の基礎ー、"日本磁気歯科学会雑誌. 8(1). 41-47 (1999)

Yasuhiro Fukui、Toshitaka Ikehara 等人：“ELF 磁场引起的牙周膜感应电流分析 - 牙槽骨再生基础”，日本磁力牙科学会杂志 8(1) (1999)。

K.H.Park, T.Ikehara, H.Yamaguchi, H.Houchi, K.K.Yoshizaki, Y.Kinouchi, and H.Miyamoto: "Effects of a time-varying magnetic field on acetylcholine-Induced transient increase in Intracellular Ca^<2+> in cultured Chromaffin cells."The Japanese Journal of the

K.H.Park、T.Ikehara、H.Yamaguchi、H.Houchi、K.K.Yoshizaki、Y.Kinouchi 和 H.Miyamoto：“时变磁场对乙酰胆碱诱导的细胞内 Ca^2 瞬时增加的影响

R.Murato, T.Ikehara, Y.Tanji, K.H.Boku, H.Kashimoto H.Houchi, K.Hosokawa, H.Yamaguchi, K.Yoshizaki, H.Miyamoto, and Y.Kinouchi: "Effects of a time-varying strong magnetic field on intracellular Ca^<2+> concentration in cultured bovine chromaffin cells."Ma

R.Murato、T.Ikehara、Y.Tanji、K.H.Boku、H.Kashimoto H.Houchi、K.Hosokawa、H.Yamaguchi、K.Yoshizaki、H.Miyamoto 和 Y.Kinouchi：“随时间变化的效应

Ikehara,T.,Park,K.H.et al: "Effects on Rb^+(K^+)uptake of HeLa cells in a high K^+ medium of exposure to a switched 1.7 Tesla magnetic field."Bioelectromagnetics. 21(in press).

Ikehara,T.,Park,K.H.等人：“在暴露于切换的 1.7 特斯拉磁场的高 K^ 介质中对 HeLa 细胞 Rb^ (K^) 吸收的影响。”生物电磁学。

K.Saito,K.Aizawa et al.: "Effects of photodynamic therapy using mono-L-aspartyl chlorin e6 on vessels and its contribution to the antitumor effect."Jpn J Cancer Res.. 91(5). 560-565 (2000)

K.Saito、K.Aizawa 等人：“使用单-L-天冬氨酰二氢卟酚 e6 的光动力疗法对血管的影响及其对抗肿瘤作用的贡献。”Jpn J Cancer Res.. 91(5)。