Effects of Magnetic field on Nerve Transmission and Analyses of Efficiency and Safety for Human on Application of Magnetic Field.

磁场对神经传递的影响及人体应用磁场的效率和安全性分析。

• 批准号: 11834009
• 负责人: IKEHARA Toshitaka
• 金额: $ 2.3万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11834009/
• 关键词: time-varying strong magnetic field; extremely-low-frequency magnetic field; intracellular Ca^<2+>; Ca^<2+>-dependent K^+ channel; endoplasmic reticulum; infrared spectroscopy; chromaffin cells; PC12 cells; イノシトール3燐酸; 誘導電流; ブラディキニン; アセチルコリン; 変動

1. The time-varying magnetic fields (MF) partly suppressed K^+ uptake through Ca^<2+>-dependent K^+ channels. When the cells were placed in the high K^+ medium, [Ca^<2+>]c increased to about 1.4 times the original level, but exposure to the magnetic fields completely suppressed the increase. Addition of ionomycin to the high K^+ medium increased [Ca^<2+>]c to the level of control cells, regardless of exposure to the magnetic field. But the inhibition of K^+ uptake by the magnetic field was not restored by addition of ionomycin. The suppression of K^+ influx could relate to a change in electric properties of cell surface.2. The time-varying MF inhibited the transient increase in [Ca^<2+>]c stimulated by acetylcholine, bradykinin, caffeine, ionomycin and thapsigargin in chromaffin cells. Also, [Ca^<2+>]c was markedly increased in inositol 1,4,5-trisphosphate-loaded cells, and this increase was inhibited by exposure. Inhibition of bradykinin-induced increase in [Ca^<2+>]c by exposure for … More 0.5 hr was mostly recovered after placing the cells for 1 hr outside the magnetic field. Our results indicate that the MF-exposure inhibits Ca^<2+> release from intracellular Ca^<2+> stores, but that bradykinin binding to bradykinin receptors of the cell membrane and inositol 1,4,5-trisphosphate production are not influenced.3. The effects of exposure to 50 Hz extremely-low-frequency (ELF) MF (maximally 41.7-43.6 mT) on structures of membrane protein of living HeLa cells were studied by the attenuated total reflection infrared spectroscopy. The exposure for 1 min shifted the peak absorbance of amide I band to a smaller wave number, strongly reduced the absorbance of amide II band and increased the absorbance at around 1600 cm^<-1>. These results suggest that exposure to the ELF magnetic field reversibly influences N-H inplane bendings and C-N stretching vibrations of peptide linkages, and changes secondary structures of alpha-helix and beta-sheet in proteins of the cell membrane.4. Exposure to ELF MF to 60 Hz MF (maximally 3 mT) further increased the differentiation of PC12 cells stimulated by addition of nerve growth factor. Less

1。时间变化的磁场（MF）部分抑制了通过Ca^<2+> - 依赖的K^+通道的K^+摄取。当将细胞放置在高K^+培养基中时，[Ca^<2+>] C增加到原始水平的约1.4倍，但是暴露于磁场会完全抑制增加。在高k^+培养基中添加离子霉素会增加[Ca^<2+>] C到对照细胞水平，而不论暴露于磁场。但是，通过添加离子霉素，无法恢复磁场对K^+摄取的抑制作用。 K^+影响的抑制可能与细胞表面的电特性变化有关2。随时间变化的MF抑制了乙酰胆碱，心动激素，咖啡因，离子霉素和Thapigargin在铬蛋白细胞中刺激的[Ca^<2+>] C的瞬时增加。同样，在1,4,4,5-三磷酸细胞中，[Ca^<2+>] C显着增加，并且暴露抑制了这种增加。在将细胞放置在磁场外1小时后，抑制缓铁蛋白诱导的[Ca^<2+>] C的增加，大多数0.5小时。我们的结果表明，MF暴露会抑制细胞内Ca^<2+>储存中的Ca^<2+>释放，但是在细胞膜和肌醇1,4,5-三磷酸磷酸盐的产生中，双阳蛋白与细胞膜和肌醇1,4,5-三磷酸的结合不受影响。3不影响3.3。暴露于50 Hz的极低频率（ELF）MF（最大41.7-43.6 MT）对活的HeLa细胞膜蛋白结构的影响通过减弱的总反射感染光谱谱研究。 1分钟的暴露使酰胺I波段的峰吸收转移到较小的波数，强烈降低了酰胺II带的吸收，并增加了在1600 cm^<-1>>大约1600 cm^<-1>上的吸收。这些结果表明，暴露于ELF磁场会可逆地影响肽链接的N-H内平面弯曲和C-N拉伸振动，并改变细胞膜蛋白中α-螺旋和β-片的二级结构。4。暴露于ELF MF至60 Hz MF（最大3吨）进一步增加了通过添加神经生长因子刺激的PC12细胞的分化。较少的

项目成果

福井康広、池原敏孝他: "ELF磁界による歯根膜誘導電流の解析ー歯槽骨再生の基礎ー、"日本磁気歯科学会雑誌. 8(1). 41-47 (1999)

Yasuhiro Fukui、Toshitaka Ikehara 等人：“ELF 磁场引起的牙周膜感应电流分析 - 牙槽骨再生基础”，日本磁力牙科学会杂志 8(1) (1999)。

R.Murato, T.Ikehara, Y.Tanji, K.H.Boku, H.Kashimoto H.Houchi, K.Hosokawa, H.Yamaguchi, K.Yoshizaki, H.Miyamoto, and Y.Kinouchi: "Effects of a time-varying strong magnetic field on intracellular Ca^<2+> concentration in cultured bovine chromaffin cells."Ma

R.Murato、T.Ikehara、Y.Tanji、K.H.Boku、H.Kashimoto H.Houchi、K.Hosokawa、H.Yamaguchi、K.Yoshizaki、H.Miyamoto 和 Y.Kinouchi：“随时间变化的效应

K.H.Park, T.Ikehara, H.Yamaguchi, H.Houchi, K.K.Yoshizaki, Y.Kinouchi, and H.Miyamoto: "Effects of a time-varying magnetic field on acetylcholine-Induced transient increase in Intracellular Ca^<2+> in cultured Chromaffin cells."The Japanese Journal of the

K.H.Park、T.Ikehara、H.Yamaguchi、H.Houchi、K.K.Yoshizaki、Y.Kinouchi 和 H.Miyamoto：“时变磁场对乙酰胆碱诱导的细胞内 Ca^2 瞬时增加的影响

Ikehara,T.,Park,K.H.et al: "Effects on Rb^+(K^+)uptake of HeLa cells in a high K^+ medium of exposure to a switched 1.7 Tesla magnetic field."Bioelectromagnetics. 21(in press).

Ikehara,T.,Park,K.H.等人：“在暴露于切换的 1.7 特斯拉磁场的高 K^ 介质中对 HeLa 细胞 Rb^ (K^) 吸收的影响。”生物电磁学。

K.Saito,K.Aizawa et al.: "Effects of photodynamic therapy using mono-L-aspartyl chlorin e6 on vessels and its contribution to the antitumor effect."Jpn J Cancer Res.. 91(5). 560-565 (2000)

K.Saito、K.Aizawa 等人：“使用单-L-天冬氨酰二氢卟酚 e6 的光动力疗法对血管的影响及其对抗肿瘤作用的贡献。”Jpn J Cancer Res.. 91(5)。