Effects of Magnetic field on Nerve Transmission and Analyses of Efficiency and Safety for Human on Application of Magnetic Field.

磁场对神经传递的影响及人体应用磁场的效率和安全性分析。

基本信息

  • 批准号:
    11834009
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2000
  • 项目状态:
    已结题

项目摘要

1. The time-varying magnetic fields (MF) partly suppressed K^+ uptake through Ca^<2+>-dependent K^+ channels. When the cells were placed in the high K^+ medium, [Ca^<2+>]c increased to about 1.4 times the original level, but exposure to the magnetic fields completely suppressed the increase. Addition of ionomycin to the high K^+ medium increased [Ca^<2+>]c to the level of control cells, regardless of exposure to the magnetic field. But the inhibition of K^+ uptake by the magnetic field was not restored by addition of ionomycin. The suppression of K^+ influx could relate to a change in electric properties of cell surface.2. The time-varying MF inhibited the transient increase in [Ca^<2+>]c stimulated by acetylcholine, bradykinin, caffeine, ionomycin and thapsigargin in chromaffin cells. Also, [Ca^<2+>]c was markedly increased in inositol 1,4,5-trisphosphate-loaded cells, and this increase was inhibited by exposure. Inhibition of bradykinin-induced increase in [Ca^<2+>]c by exposure for … More 0.5 hr was mostly recovered after placing the cells for 1 hr outside the magnetic field. Our results indicate that the MF-exposure inhibits Ca^<2+> release from intracellular Ca^<2+> stores, but that bradykinin binding to bradykinin receptors of the cell membrane and inositol 1,4,5-trisphosphate production are not influenced.3. The effects of exposure to 50 Hz extremely-low-frequency (ELF) MF (maximally 41.7-43.6 mT) on structures of membrane protein of living HeLa cells were studied by the attenuated total reflection infrared spectroscopy. The exposure for 1 min shifted the peak absorbance of amide I band to a smaller wave number, strongly reduced the absorbance of amide II band and increased the absorbance at around 1600 cm^<-1>. These results suggest that exposure to the ELF magnetic field reversibly influences N-H inplane bendings and C-N stretching vibrations of peptide linkages, and changes secondary structures of alpha-helix and beta-sheet in proteins of the cell membrane.4. Exposure to ELF MF to 60 Hz MF (maximally 3 mT) further increased the differentiation of PC12 cells stimulated by addition of nerve growth factor. Less
1.时变磁场部分抑制了依赖于Ca~(2+)和Gt~(2+)的K~(++)通道对K~+的吸收。当细胞置于高K^+培养液中时,[Ca^&lt;2+&gt;]c增加到原来的1.4倍左右,但暴露在磁场中完全抑制了这种增加。在高K^+培养液中加入离子霉素使[Ca^&lt;2+&gt;]c增加到对照细胞的水平,与磁场暴露无关。但外加离子霉素不能恢复磁场对K+吸收的抑制作用。K~+内流的抑制可能与细胞表面电学性质的改变有关。时变MF抑制乙酰胆碱、缓激肽、咖啡因、离子霉素和thapsigargin刺激的嗜铬细胞[Ca~(2+)]_c的一过性升高。此外,在1,4,5-三磷酸肌醇负载的细胞中,[Ca~(2+)&t;2+&gt;]c显著升高,这种升高可被暴露所抑制。…抑制缓激肽诱导的[Ca~(2+)]_c升高在磁场外放置1小时后,细胞大部分恢复到0.5小时以上。我们的结果表明,磁场暴露抑制了细胞内钙离子的释放,但不影响缓激肽与细胞膜上缓激肽受体的结合以及1,4,5-三磷酸肌醇的产生。用衰减全反射红外光谱仪研究了50赫兹极低频(ELF)磁场(最大41.7-43.6mT)对活体HeLa细胞膜蛋白结构的影响。暴露1min使酰胺I带的峰值吸光度移动到一个较小的波数,显著降低了酰胺II带的吸光度,并在1600 cm~(-1)~(-1)处增大了吸光度。这些结果表明,暴露在ELF磁场中可逆地影响了多肽键的N-H面内弯曲和C-N伸缩振动,并改变了细胞膜蛋白质中α-螺旋和β-折叠的二级结构。暴露于60赫兹磁场(最大3mT)的极低频磁场进一步增加了由神经生长因子刺激的PC12细胞的分化。较少

项目成果

期刊论文数量(25)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
福井康広、池原敏孝他: "ELF磁界による歯根膜誘導電流の解析ー歯槽骨再生の基礎ー、"日本磁気歯科学会雑誌. 8(1). 41-47 (1999)
Yasuhiro Fukui、Toshitaka Ikehara 等人:“ELF 磁场引起的牙周膜感应电流分析 - 牙槽骨再生基础”,日本磁力牙科学会杂志 8(1) (1999)。
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    0
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K.H.Park, T.Ikehara, H.Yamaguchi, H.Houchi, K.K.Yoshizaki, Y.Kinouchi, and H.Miyamoto: "Effects of a time-varying magnetic field on acetylcholine-Induced transient increase in Intracellular Ca^<2+> in cultured Chromaffin cells."The Japanese Journal of the
K.H.Park、T.Ikehara、H.Yamaguchi、H.Houchi、K.K.Yoshizaki、Y.Kinouchi 和 H.Miyamoto:“时变磁场对乙酰胆碱诱导的细胞内 Ca^2 瞬时增加的影响
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R.Murato, T.Ikehara, Y.Tanji, K.H.Boku, H.Kashimoto H.Houchi, K.Hosokawa, H.Yamaguchi, K.Yoshizaki, H.Miyamoto, and Y.Kinouchi: "Effects of a time-varying strong magnetic field on intracellular Ca^<2+> concentration in cultured bovine chromaffin cells."Ma
R.Murato、T.Ikehara、Y.Tanji、K.H.Boku、H.Kashimoto H.Houchi、K.Hosokawa、H.Yamaguchi、K.Yoshizaki、H.Miyamoto 和 Y.Kinouchi:“随时间变化的效应
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    0
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Ikehara,T.,Park,K.H.et al: "Effects on Rb^+(K^+)uptake of HeLa cells in a high K^+ medium of exposure to a switched 1.7 Tesla magnetic field."Bioelectromagnetics. 21(in press).
Ikehara,T.,Park,K.H.等人:“在暴露于切换的 1.7 特斯拉磁场的高 K^ 介质中对 HeLa 细胞 Rb^ (K^) 吸收的影响。”生物电磁学。
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    0
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K.Saito,K.Aizawa et al.: "Effects of photodynamic therapy using mono-L-aspartyl chlorin e6 on vessels and its contribution to the antitumor effect."Jpn J Cancer Res.. 91(5). 560-565 (2000)
K.Saito、K.Aizawa 等人:“使用单-L-天冬氨酰二氢卟酚 e6 的光动力疗法对血管的影响及其对抗肿瘤作用的贡献。”Jpn J Cancer Res.. 91(5)。
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IKEHARA Toshitaka其他文献

IKEHARA Toshitaka的其他文献

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{{ truncateString('IKEHARA Toshitaka', 18)}}的其他基金

Effects of radio-frequency electromagnetic field on physiological functions of nerve cells
射频电磁场对神经细胞生理功能的影响
  • 批准号:
    19510032
  • 财政年份:
    2007
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Effects of magnetic fields on many functions of human and other mammalian cells and proposal of guidelines and Standard for Human Health
磁场对人类和其他哺乳动物细胞许多功能的影响以及人类健康指南和标准的提议
  • 批准号:
    12558062
  • 财政年份:
    2000
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of inhibitory mechanisms on Ca^<2+>-dependent K^+ channel influenced by exposure to a time-varying strong magnetic field.
分析时变强磁场暴露对Ca^2依赖性K^通道的抑制机制。
  • 批准号:
    09680526
  • 财政年份:
    1997
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Evaluation of simulative effect of adrenal steroids secretion by exposure to an extremely-low-frequency magnetic field.
通过暴露于极低频磁场来评估肾上腺类固醇分泌的模拟效果。
  • 批准号:
    25870475
  • 财政年份:
    2013
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
The effect of a specific pulsed extremely low frequency magnetic field on thermal nociception in humans
特定脉冲极低频磁场对人类热伤害感受的影响
  • 批准号:
    278551-2003
  • 财政年份:
    2003
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Development of a newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic field
开发新设计的实验系统,将哺乳动物细胞暴露于极低频磁场中
  • 批准号:
    04559006
  • 财政年份:
    1992
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
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