Development of a newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic field

开发新设计的实验系统，将哺乳动物细胞暴露于极低频磁场中

• 批准号: 04559006
• 负责人: MIYAKOSHI Junji
• 金额: $ 6.59万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04559006/
• 关键词: Extremely Low Frequency Magnetic Field; Cultured Mammalian Cells; Gene Expression; beta-galactosidase; Signal Transduction; Cyclic AMP; Protein Kinase C; プロテインカイネースC; 磁石; 放射線感受性; 情報伝達

To examine the biological effects of extremely low frequency magnetic field (ELFMF), we have designed and manufactured a new equipment for long-term and high density exposure of cells to ELFMF.The ELFMF exposure system consists of a generator of magnets with a bult-in CO_2 incubator, an alternating current power supply, a gas compressor and thermocontroller for the incubator, and a cooling unit for the magnets. The maximum magnet flux density on the exposure area of the incubator is 500 mT (tesla) at a current of 556 Arms at 50 Hz. The effect of ELFMF on gene expression was investigated using PCVG cells which were transfected with the beta-galactosidase (beta-gal.) gene expression plasmid in the parental PC12 cells. The activity of beta-gal. was very low without stimulation. The treatment with forskolin (2muM) enhanced the beta-gal. activity. The activity was further enhanced by the combined exposure with forskolin and 400 mT ELFMF.The stimulation by forskolin and TPA (25ng/ml) induced 2.5-fold increase in the beta-gal. activity. The activity was further enhanced under exposure of ELFMF.From these results, the exposure of ELFMF may stimulate the intracellular signal transduction resulting in the beta-gal. expression in PCVG cells.

为了研究极低频磁场（ELFMF）的生物效应，我们设计并制造了一种新型的细胞ELFMF长期高密度暴露装置，该装置包括一个带内置式CO_2培养箱的磁体发生器、一个交流电源、培养箱的气体压缩机和温控器以及磁体冷却装置。在50 Hz、556 Arms的电流下，培养箱暴露区域上的最大磁通密度为500 mT（特斯拉）。使用用β-半乳糖苷酶（β-gal.）转染的PCVG细胞研究ELFMF对基因表达的影响。在亲本PC 12细胞中的基因表达质粒。β-半乳糖的活性。在没有刺激的情况下非常低。用毛喉素（2 μ M）处理增强了β-gal。活动Forskolin和400 mT ELFMF联合作用可进一步增强β-gal的活性，其中forskolin和TPA（25 ng/ml）联合作用可使β-gal增加2.5倍。活动结果表明，ELFMF的作用可能是通过刺激细胞内信号转导而导致β-gal的产生。在PCVG细胞中表达。

项目成果

Junji Miyakoshi: "A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic field." Journal of Radiation Research. (in press).

Junji Miyakoshi：“一种新设计的实验系统，用于将哺乳动物细胞暴露于极低频磁场中。”

Junji Miyakoshi, Shuji Ohtsu, Junko Tatsumi-Miyajima, and Hiraku Takebe: "A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic field." Journal of Radiation Research. (in press).

Junji Miyakoshi、Shuji Ohtsu、Junko Tatsumi-Miyajima 和 Hiraku Takebe：“一种新设计的实验系统，用于将哺乳动物细胞暴露于极低频磁场。”