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Expression of Neurotrophins After Experimental Spinal Cord Injury

实验性脊髓损伤后神经营养素的表达

基本信息

批准号：
11835009
负责人：
YOSHINAGA Kasunori
金额：
$ 2.11万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11835009/
关键词：
Spinal cord injury brain-derived neurotrophic factor neurotrophin-3 neurotrophin-3(NT-3)

项目摘要

BDNF and NT-3, members of the NGF family of trophic factors, have multiple functions including a role in the promotion of neuronal survival and nerve fiber elongation in both the CNS and the PNS.We assessed the expression of endogenous BDNF and NT-3 following an experimentally-induced compression injury to the spinal cord. Expression of BDNF mRNA was increased following the spinal cord injury ; reaching maximum levels 24 hr after the injury. Expression of BDNF mRNA returned to the levels observed in sham-operated control antimals within 3 days of the injury. In contrast, NT-3 mRNA was up-regulated 1 week after the injury and reached to the peak level 2 weeks after the trauma. Using the in situ hybridization technique, we observed a wide distribution of BDNF and NT-3 expression amongst the different cell types in the spinal cord including motor and sensory neurons, and in glial cells, including astrocytes and oligodendrocytes. These results suggest that BDNF is synthesized in both neurons and glial cells during the acute response to injury to the spinal cord, functioning in a mainly neuroprotective role. This is followed by a later phase of NT-3 expression in neurons and glial cells, functioning in a restorative capacity. Especially, NT-3 expression in oligodendrocytes in the later phase suggests that this factor supports restration of long tracts.

BDNF和NT-3，营养因子的NGF家族的成员，具有多种功能，包括在促进神经元的存活和神经纤维的伸长在CNS和PNS的作用。我们评估了内源性BDNF和NT-3的表达后，实验诱导的脊髓压迫损伤。脊髓损伤后BDNF mRNA的表达增加，在损伤后24小时达到最高水平。BDNF mRNA的表达在损伤后3天内恢复到假手术对照组的水平。而NT-3 mRNA在伤后1周即开始表达上调，2周达高峰。使用原位杂交技术，我们观察到广泛分布的BDNF和NT-3表达的不同类型的细胞在脊髓中，包括运动和感觉神经元，和神经胶质细胞，包括星形胶质细胞和少突胶质细胞。这些结果表明，BDNF在脊髓损伤的急性反应中在神经元和胶质细胞中合成，主要起神经保护作用。随后是神经元和神经胶质细胞中NT-3表达的后期阶段，以恢复能力发挥作用。特别是，NT-3在少突胶质细胞中的表达在后期表明，该因子支持长束的抑制。