The immunohistochemical analysis in the mouse stroke model.

小鼠卒中模型中的免疫组织化学分析。

• 批准号: 11835049
• 负责人: YAMANO Mariko
• 金额: $ 1.41万
• 依托单位: Osaka Prefecture College of Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11835049/
• 关键词: Pyramidal tract lesion; immunohistochemistry; c-fos; GFAP; central nervous system; muscle; 脳卒中; 脳; 脳卒中モデル動物; 神経活性物質; c-fos; CaM Kinase II

(1) We made stroke model of mouse which were received hemi electrically lesion of pyramidal tract and examined the immunohistochemical changes in the central nervous system and muscles in these animals.(2) Only few c-fos immunoreactivity (IR) were occurred in the rest animals but after treadmill running, rich c-fos-IR nuclei were distributed in the CNS.Glial fibrillary acidic protein (GFAP)-IR and CaM kinase II-IR were a few in the CNS both rest and running animals.(3) In the lesioned animals many changes were occurred. Very rich GFAP-IR and CaM kinase II (CaM II)-IR were seen widely in the operated side. After 30min treadmill running, c-fos-IR were not found in the lesion site especially GFAP-IR and CaMII-IR were rich areas at day 4 and day 7 after surgery. These findings suggest that reactive astrocytes may depressed the neuronal activity at early recovery stage. At day 14 to 1 month, c-fos-IR were gradually increased and GFAP were decreased in the lesioned site.(4) In the muscles, few c-fos-IR nuclei were occurred in the palaritic side on the contrary, many c-fos-IR nuclei were occurred in the normal side at day 4 to day 7. These findings suggest that opposite muscles may enlarged compensation to the palaritic side at early recovery stage.

(1)采用半电损毁锥体束的方法建立小鼠脑卒中模型，观察脑卒中后中枢神经系统和肌肉的免疫组化变化。(2)运动后，c-fos-IR核在中枢神经系统内分布丰富，GFAP-IR和CaM激酶II-IR在运动和安静时均为少数。(3)在损伤的动物中发生了许多变化。手术侧广泛分布着丰富的GFAP-IR和CaM激酶II（CaM II）-IR。运动30 min后，病灶区未见c-fos-IR，尤其是术后第4、7天GFAP-IR和CaMII-IR较丰富。这些结果表明，反应性星形胶质细胞可能在恢复早期抑制神经元的活动。14 ~ 1个月时，损伤区c-fos-IR逐渐升高，GFAP逐渐降低。(4)第4 ~ 7天，患侧肌肉中c-fos-IR阳性细胞数较少，而正常侧肌肉中c-fos-IR阳性细胞数较多。提示在恢复早期，对侧肌群可能对患侧有代偿作用。

项目成果

Mariko Yamano: "C-fos expression during experimental stroke in the mice."Acta Anatomica Nipponica. VOL.76-1. 88 (2001)

Mariko Yamano：“小鼠实验性中风期间 C-fos 的表达。”Acta Anatomica Nipponica。

Mariko Yamano: "C-fos expression during exercise in the mice II."Acta Anatomica Nipponica. VOL.75-1. 105 (2000)

Mariko Yamano：“小鼠 II 运动期间 C-fos 的表达。”Acta Anatomica Nipponica。

山野眞利子: "運動とc-fos蛋白の動態についてII"Acta Anatomica Nipponica. 75-1. 105 (2000)

Mariko Yamano：“c-fos 蛋白 II 的运动和动力学”Acta Anatomica Nipponica 75-1 (2000)。

山野眞利子: "錐体路破壊動物の筋および中枢神経系の動態について"Acta Anatomica Nipponica. 76-1. 88 (2001)

Mariko Yamano：“锥体束破坏动物的肌肉和中枢神经系统动力学”Acta Anatomica Nipponica 76-1（2001）。