Effects of endocrine disrupters on function of thyroid gland, adrenal glands and gonads

内分泌干​​扰物对甲状腺、肾上腺和性腺功能的影响

• 批准号: 11839003
• 负责人: TAYA Kazuyoshi
• 金额: $ 2.3万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11839003/
• 关键词: Endocrine disrupter; Bisphenol A; Octylphenol; Genistein; testosterone; inhibin; Prolactin; rats; シャトルボックス回避能; HAA、LAAラット; Hatanoラット; 内分泌撹乱作用; 春機発動; 自発運動量; 免疫機能; 精子形成

The present study investigated the effects of a xenobiotic estrogen, Bisphenol A(BPA)and Octylphenol(OP)on reproductive functions using male and female rats. Results indicated that BPA and OP directly inhibit testicular functions. The testis is probably a more sensitive site for BPA and OP actions than the hypothalamus-pituitary axis. On the other hand, the exposure of neonatal female rats to higher doses of OP disrupts the cyclic release of LH, FSH and prolactin, and interferes with the display of sexual receptive behavior in adulthood.The Hatano high- and low-avoidance animals(HAAs and LAAs), selectively bred from Sprague-Dawley rats, are characterized as an intra-strain dissociation in avoidance learning, locomotor activity, puberty, estrous cycles, sperm motility, hypothalamo-pituitary-adrenal(HPA)axis and immune response. The present study was therefore designed to compare the effects of lactational exposure to genistein, a phytoestrogen, on those functions between HAAs and LAAs. … More To this aim, litters from both strains were fostered onto Sprague-Dawley rats, which were administered from gestational day 17 to postnatal day 21. In LAAs, puberty was significantly delayed in the both sexes of offspring reared by genistein-treated dams compared with those by vehicle-treated dams, whereas no significant effects of genistein on puberty were observed in HAAs. Open field activity was significantly increased in male HAAs that reared by genistein-treated dams. The number of lymphocytes and antibody production response to sheep red blood cells were significantly decreased in HAAs reared by genistein-treated dams. No genistein-related but strain-related differences were detected in estrous cycling, learning behavior, sperm motility and weight of reproductive organs. These results demonstrated that genistein has endocrine disrupting effects on behavior, reproduction and immune response of rats when exposures during lactation. These results also indicated that there are clear differences of endocrine disrupting effects in two genetically selected strain rats. Less

本文研究了外源雌激素双酚a （BPA）和辛基酚（OP）对雌雄大鼠生殖功能的影响。结果表明，BPA和OP直接抑制睾丸功能。睾丸对BPA和OP的作用可能比下丘脑-垂体轴更敏感。另一方面，将新生雌性大鼠暴露于高剂量的OP中，会破坏黄体生成素、卵泡刺激素和催乳素的循环释放，并干扰成年后的性接受行为。从Sprague-Dawley大鼠中选择性培育的Hatano高、低回避动物（HAAs和LAAs）在回避学习、运动活动、青春期、发情周期、精子活力、下丘脑-垂体-肾上腺（HPA）轴和免疫反应方面表现出菌株内解离。因此，本研究旨在比较哺乳期暴露于染料木素（一种植物雌激素）对HAAs和LAAs之间这些功能的影响。为此，两种菌株的幼崽都被培养到Sprague-Dawley大鼠身上，从妊娠第17天到出生后第21天。在LAAs中，染料木素处理的坝与车辆处理的坝相比，雌雄后代的青春期明显推迟，而在HAAs中没有观察到染料木素对青春期的显著影响。用染料木黄酮处理的坝饲养的雄性HAAs，其野外活动显著增加。用染料木黄酮处理的母鼠饲养的haa淋巴细胞数量和对绵羊红细胞的抗体产生反应显著降低。在发情周期、学习行为、精子活力和生殖器官重量等方面均未发现与染料木素相关的差异，但与品系相关。这些结果表明，染料木素在哺乳期暴露对大鼠的行为、繁殖和免疫反应具有内分泌干扰作用。这些结果也表明，两种遗传选择品系大鼠的内分泌干扰作用存在明显差异。少

项目成果

Heratl, C. B.: "Exposure of neonatal female rats to p-tert-octylphenol disrupts afternoon surges of LH, FSH and prolactin secretion, and interferes with sexual receptive"Biology of Reproduction. 64. 1216-1224 (2001)

Heratl, C. B.：“新生雌性大鼠接触对叔辛基苯酚会扰乱午后 LH、FSH 和催乳素分泌的激增，并干扰性接受”《生殖生物学》。

Tohei, A.: "Hypothyroidism increases prolactin secretion and decreases the intromission threshold for induction of pseudopregnancy in adult female rats"Physicology and Beharion. 69. 391-397 (2000)

Tohei, A.：“甲状腺功能减退症会增加催乳素的分泌，并降低成年雌性大鼠假孕诱导的插入阈值”《生理学》和《Beharion》。

Katsuda,S.,Yoshida,M.,Watanabe,G.Taya,K.and Maekawa,A.: "Irreversible effects of neonatal exposure to P-test-octylPkansl on the reproductive tract in female rats."Taxicology and Applied Pharmacology. 165. 217-226 (2000)

Katsuda,S.、Yoshida,M.、Watanabe,G.Taya,K. 和 Maekawa,A.：“新生儿接触 P-test-octylPkansl 对雌性大鼠生殖道的不可逆影响。”毒理学和应用药理学。

Tohei, A.: "Bisphenol A inhibits teotiarlar function and inneases LH seaetion in adult male rats"The Pruceedirgs of the Soaety for Experinental Biology and Medicino. 226. 216-221 (2001)

Tohei, A.：“双酚 A 抑制成年雄性大鼠的足部功能并促进 LH 分泌”实验生物学和医学协会的规程。

Tohei A., Suda S., Taya K., Hashimoto T.and Kogo H.: "Bisphenol A inhibits testicular function and increases LH secretion in adult male rats."The Proceedings of the Society for Experimental Biology and Medicine. 226. 216-221 (2001)

Tohei A.、Suda S.、Taya K.、Hashimoto T. 和 Kogo H.：“双酚 A 抑制成年雄性大鼠的睾丸功能并增加 LH 分泌。”实验生物学与医学学会会刊。