Effect of Environmental Endocrine Disrupters on pancreatic hormones secretion

环境内分泌干扰物对胰腺激素分泌的影响

• 批准号: 11839012
• 负责人: TSUDA Kinsuke
• 金额: $ 2.18万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11839012/
• 关键词: Enviromental Endocrine Disrupters; Bisphenol-A; Nonylphenol; Insulin; 環境ホルモン

We pay attention Environmental Endocrine Disrupters (EEDs).Some of them have the actions like estrogen. Estrogen is important for development or propagation. It has many other actions ; lipid metabolism, bone metabolism and so on, but it is not clear whether estrogen has any action on pancreatic hormone secretion. On the other hand patients with diabetes mellitus increase rapidly in Japan. We make a hypothesis that EEDs may any relation with the increase of diabetes mellitus.So to clarify whether some of EEDs have any effect on insulin secretion we studied the effects of Bisphenol A and Nonylphenol by using rat isolated pancreatic islets.Our data suggest that the pancreatic islets have the estrogen receptor (α type and β type). We also suggest that Bisphenol A and Nonylphenol have the insulinotropic actions using isolated pancreatic islets examinations.

我们关注环境内分泌干扰物（eed）。其中一些有雌激素的作用。雌激素对发育和繁殖很重要。它还有许多其他的动作；脂质代谢、骨代谢等，但雌激素对胰腺激素分泌是否有作用尚不清楚。另一方面，糖尿病患者在日本迅速增加。我们提出了一个假设，即eed可能与糖尿病的增加有关。因此，为了弄清某些eed是否对胰岛素分泌有影响，我们利用大鼠离体胰岛研究了双酚A和壬基酚的作用。我们的数据表明，胰岛有雌激素受体（α型和β型）。我们还建议双酚A和壬基酚通过离体胰岛检查具有胰岛素调节作用。

项目成果

K.Nunoi,K.Yanida K.Tsuda et al: "Wortmannin. a P13-kinase indilution : Prinoting effect on ioudu noustion from pancneation cells thash a CAMP-dependent prthway "Biochem Biophys.Res.Comman. 270. 798-805 (2000)

K.Nunoi、K.Yanida K.Tsuda 等人：“Wortmannin。P13 激酶稀释液：对来自 panneation 细胞的 ioudu noustion 的打印效应，thash 依赖于 CAMP 的通道”Biochem Biophys.Res.Comman。

A.Inada,K,Tsuda.et al.: "The cyclic AMP response element modulator family regulates the Insulin gene transcription by interacting with transcription factor 2D"J.Biol.Chem. 274(30). 21095-21103 (1999)

A.Inada,K,Tsuda.et al.：“环 AMP 反应元件调节剂家族通过与转录因子 2D 相互作用来调节胰岛素基因转录”J.Biol.Chem。

N.Shihara,K.Yasuda,K.Tsuda, et al.: "The association between Trp64 Arg polymorphism of the β3-adrenergic receptor and autonomic nervous system activity."J.Clinical Endocrinol.Metab.. 84. 1623-1627 (1999)

N.Shihara、K.Yasuda、K.Tsuda 等人：“β3-肾上腺素受体 Trp64 Arg 多态性与自主神经系统活动之间的关联。”J.Clinical Endocrinol.Metab.. 84. 1623-1627 ( 1999）

N.Shihara, K.Yasuda, , K.Tsuda, et al.: "The association between Trp64 Arg polymorphism of the β 3-adrenergicreceptor and autonomicnervous system activity."J.Clinical Endocrinol.Metab.. 84(5). 1623-1627 (1999)

N.Shihara、K.Yasuda、K.Tsuda 等人：“β 3-肾上腺素受体 Trp64 Arg 多态性与自主神经系统活动之间的关联。”J.Clinical Endocrinol.Metab.. 84(5)。 1623 -1627 (1999)

K.Nunoi, K.Yasuda, K.Tsuda et al.: "Wortmanin, a PI3-kinase inhibitor : promoting effect on insulin secretion from pancreaticbeta cells through a cAMP-dependentpathway Biochem. Biophy."Res.Comm.. 270(3). 798-805 (2000)

K.Nunoi、K.Yasuda、K.Tsuda 等人：“Wortmanin，一种 PI3 激酶抑制剂：通过 cAMP 依赖性途径 Biochem. Biophy 促进胰腺β细胞分泌胰岛素。”Res.Comm.. 270(3