Study on the relationship between NHE and diabetic nephropathy.

阐明糖尿病肾病的发病机制和高危因素具有重要意义。我们测量了红细胞钠-锂反向转运活性（SLI），这被认为是相同的钠-质子交换剂（NHE），我们的数据表明，SLC强烈反映了高血压的易感性，它可以是一个有用的标记糖尿病肾病。最近，NHE的四种异构体被克隆和测序。我们证明了所有NHE异构体都存在于肾脏中，并且在GK大鼠（2型糖尿病模型）中NHE 3的表达高于对照大鼠。我们还阐明了NHE通过Na离子调节细胞内Ca离子浓度。现尝试建立NHE基因异常检测系统。

It is very important to clarify the pathogenesis and predictor of high risk for diabetic nephropathy. We have measured erythrocyte sodium-lithium-countertransport activity(SLI) which is thought to be identical sodium-proton exchanger(NHE) and our data suggest that SLC strongly reflects a predisposition to hypertension and that it can be a useful marker of diabetic nephropathy. Recently four isoforms of NHE are cloned and sequenced. We demonstrated that all NHE isoforms exist in kidney and in GK rats (type 2 diabetes model) NHE3 are expressed more than the control rats. We also clarified that NHE regulates intracellular Ca ion concentrations through Na ion. Now try to establish the system for detecting abnormality of NHE gene.