INFLOUENCE OF ENDOCREINE DISRUPTORS ON EMBRYO AND FRY OF VIVIPAROUS FISH.

内分泌干​​扰物对胎生鱼胚胎和鱼苗的影响。

• 批准号: 11839019
• 负责人: SOYANO Kiyoshi
• 金额: $ 2.18万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11839019/
• 关键词: ENDOCRINE DESRUPTOR; VIVIPAROUS FISH; GONADAL ABNORMALITY; ESTROGEN; ビスフェノールA; 雄性ホルモン

There is little information of endocrine disruptors in viviparous fish. The purpose of the present study was to investigate the influence of environmental estrogens on embryo and fry of mosquitofish, viviparous fish, using bisphenol A(BPA) which is found to exert estrogenic effects in fish. Females with ovulated eggs and pregnant females were exposed to BPA at concentration of 20ppb and reared until the parturition of fry. The mortality and the abnormality of newborn fry which was bred by BPA exposed pregnant females were examined. The stillbirth(92.1%) was induced by BPA exposure. The deformation of head, the curvature of vertebra and the hypertrophy of yolk sac were observed as abnormalities in all the stillborn fry. Newborn fry bred by normal females were exposed to BPA at the same concentration for 10, 20 or 30 days after the parturition. In this experiment, the mortality of exposed fry was not significantly different from control fry and the abnormality was not observed. These results suggest that environmental estrogens may influence the embryogenesis in viviparous fish.

关于胎生鱼体内内分泌干扰物的信息很少。本研究的目的是探讨环境雌激素对鱼胚胎和鱼苗的影响，使用双酚A（BPA），发现在鱼类中发挥雌激素效应。将产卵的雌鱼和怀孕的雌鱼暴露于浓度为20ppb的BPA中，并饲养至鱼苗分娩。对BPA暴露的孕母鱼所培育的新生鱼苗的死亡率和畸形率进行了检测。死胎（92.1%）是由BPA暴露引起的。所有死产鱼苗均出现头部变形、脊椎弯曲和卵黄囊肥大等异常。将正常雌鱼培育的新生鱼苗在分娩后分别暴露于相同浓度的BPA中10、20和30天。在本试验中，暴露鱼苗的死亡率与对照鱼苗无显著差异，未观察到异常。这些结果表明，环境雌激素可能影响胎生鱼胚胎发生。

项目成果

H.Ishibashi, K.Tachibana, M.Tsuchimoto, K.Soyano, Y.Ishibashi, M.Nagae, S.Kohra, Y.Takao, N.Tominaga, and K.Arizono: "In vivo testing system for determining the estrogenic activity of endocrine disrupting chemicals(EDCs) in goldfish(Carassius auratus)"Jou

H.Ishibashi、K.Tachibana、M.Tsuchimoto、K.Soyano、Y.Ishibashi、M.Nagae、S.Kohra、Y.Takao、N.Tominaga 和 K.Arizono：“用于测定雌激素的体内测试系统

Senoo,Daisuke et al: "The influence of bisphenol A on embryo and fry of mosquito fish (Gambusia affinis)"Environment Sciences. 8. - (2001)

Senoo、Daisuke 等人：“双酚 A 对食蚊鱼 (Gambusia affinis) 胚胎和鱼苗的影响”环境科学。

Ishibashi,Hiroshi et al: "In Vivo testing system for determining the estrogenic activity of endocrine disrupting chemicals (EDCs) in goldfish."Journal of Health Science. 47. 213-218 (2001)

Ishibashi, Hiroshi 等人：“用于测定金鱼内分泌干扰化学物质 (EDC) 雌激素活性的体内测试系统。”健康科学杂志。

D.Senoo: "The influence of bisphenol A on early development of mosquitofish(Gambusia affinis)."Master's thesis, Nagasaki University. (2001)

D.Senoo：“双酚A对食蚊鱼（Gambusia affinis）早期发育的影响。”硕士论文，长崎大学。

D.Senoo, I.Takahashi, H.C.Lee, Y.Koya, Y.Takao, M.Nagae, and k.Soyano: "The influence of bisphenol A on embryo and fry of mosquitofish(Gambusia affinis)"Environment Sciences. 8. (2001)

D.Senoo、I.Takahashi、H.C.Lee、Y.Koya、Y.Takao、M.Nagae 和 k.Soyano：“双酚 A 对食蚊鱼（Gambusia affinis）胚胎和鱼苗的影响”环境科学。