Survey of estrogenic contamination in the costal waters of East China Sea using grey mullet.

东海近海灰鲻鱼雌激素污染调查.

• 批准号: 15405005
• 负责人: SOYANO Kiyoshi
• 金额: $ 7.81万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15405005/
• 关键词: Endocrine disruption; grey mullet; abnormality; vitellogenin; East China Sea

It is well known that endocrine and sexual disruptions in wild teleosts are induced by endocrine disrupting chemicals which have an estrogen-like function. However, the actualities of contamination by these substances in aquatic environment of coastal waters are not investigated well in Japan and East Asia. We carried out the monitoring of environmental estrogens in marine and estuarine areas using wild grey mullet (Mugil cephalus). In this research, we tried to determine the plasma concentrations of vitellogenin (VTG) which is the major yolk protein produced by the liver under the influence of estrogen. Moreover, we observed gonadal abnormalities using histological technique.VTG levels of grey mullets in the bay areas were significantly higher than the ones of fish collected in the open sea. In male and immature female fish of Tokyo Bay, Osaka Bay, Japan, VTG levels were the same as mature female fish. These areas are fed by a large amount of industrial and domestic sewage and effluent. VTG level is generally very low (<1μg/ml) in male and immature fish, because vitellogenic oocytes are not included in the gonad of these fish. These results indicate that VTG synthesis was induced by exogenous estrogen-like substance (environmental estrogen). VTG levels of the fish collected from South part of Korea were higher than another place of Korea.We will start the new survey of estrogenic contaminations in costal waters in the East China Sea with scientist of Korea and China. Moreover, we have a plan to construct the network of scientist for the research of endocrine disruption in East Asia.

众所周知，野生硬骨鱼的内分泌和性干扰是由具有类雌激素功能的内分泌干扰化学物质引起的。然而，在日本和东亚地区，这些物质对沿海水域水环境的污染现状还没有得到很好的调查。我们利用野生鲻鱼（Mugil cephalus）对海洋和河口地区的环境雌激素进行了监测。在这项研究中，我们试图测定卵黄蛋白原 (VTG) 的血浆浓度，VTG 是肝脏在雌激素影响下产生的主要卵黄蛋白。此外，我们还利用组织学技术观察到性腺异常。海湾地区灰鲻鱼的VTG水平显着高于外海采集的鱼类。日本东京湾、大阪湾的雄鱼和未成熟雌鱼中，VTG 水平与成熟雌鱼相同。这些地区有大量的工业和生活污水和废水。雄性和未成熟鱼中的 VTG 水平通常非常低（<1μg/ml），因为这些鱼的性腺中不包含卵黄发生卵母细胞。这些结果表明VTG合成是由外源性雌激素样物质（环境雌激素）诱导的。从韩国南部采集的鱼的VTG水平高于韩国其他地方。我们将与韩国和中国的科学家一起开始东海沿岸水域雌激素污染的新调查。此外，我们还计划建立东亚内分泌干扰研究科学家网络。

项目成果

Estrogenic potency of a bilphenol A metabolite on vitellogenin synthesis in Medaka,Oryzias latipes.

双酚 A 代谢物对青鳉、Oryzias latipes 卵黄蛋白原合成的雌激素效力。

• 发表时间: 2005
• 期刊: Journal of Health Science 51
• 作者: Nagae;Masaki
• 通讯作者: Masaki

High sensitivity analysis of indirubin by silyration using GC/MS.

使用 GC/MS 通过硅烷化对靛玉红进行高灵敏度分析。

• 发表时间: 2003
• 期刊: Journal of Health Science 49
• 作者: Takao;Yuji
• 通讯作者: Yuji

Gonadal histology and serum vitellogenin levels of bigeye tuna Thunnus obesus from the Northern Pacific Ocean--absence of endocrine disruption bio-indicators.

• DOI: 10.1016/s0025-326x(02)00460-5
• 发表时间: 2003-04
• 期刊: Marine pollution bulletin
• 影响因子: 5.8
• 作者: S. Hashimoto;R. Kurihara;C. A. Strüssmann;T. Yamasaki;K. Soyano;A. Hara;H. Shiraishi;M. Morita

微量人工化学物質の生物モニタリング(水産学シリーズ)(沿岸性魚類を執筆)

痕量人工化学品的生物监测（渔业科学丛书）（写于沿海鱼类）

• 发表时间: 2004
• 作者: Matsubara;Takahiro et al.;原 彰彦
• 通讯作者: 原 彰彦

Multiple vitellogenins and their unique roles in marine teleosts

• DOI: 10.1023/b:fish.0000030559.71954.37
• 发表时间: 2003-01-01
• 期刊: FISH PHYSIOLOGY AND BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Matsubara, T;Nagae, M;Hara, A
• 通讯作者: Hara, A