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Study on the mechanism of lunar-related spawning in honeycomb grouper

蜂窝石斑鱼涉月产卵机制研究

基本信息

批准号：
18580186
负责人：
SOYANO Kiyoshi
金额：
$ 2.43万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580186/
关键词：
maturation lunar-related spawning honeycomb grouper hormone spawning migration biotelemetry

项目摘要

The honeycomb grouper ainephelus merra, which is distributed in the sub-tropical and tropical area in Pacific Ocean, is known as a lunar-related spawning. However, details of gonadal development and spawning in this species are not well understood. In this study, we investigated the gonadal development, hormonal changes, and expression of gonadotropin gene in female collected from the reef area around Sesoko Island and Iriomote Island in Okinawa during spawning season. In addition, we investigated the spawning migration to suitable environment for spawning using biotelemetry method.Ovaries of fish collected in both areas were occupied with oocytes of the tertiary yolk stage, when gonadosomatic index increased and reached the maximum level around full moon. However, mature size of fish in Ireornete Island was smaller than that in Sesoko Island. Moreover, fish of Treomote started female-to-male sex change immediately after the spawning season. On the other hand, sex change in fish collected from Sesoko Island stared in a few months after the spawning season. The different characteristics in both areas may depend on the difference in density, niche in the reefs, depth, water current, and water temperature. Estradiol levels indicated high just prior to spawning. Luteinizing hormone beta subunit gene expressed during reproductive period in female grouper, suggesting LH has important roles on gonadal development and steroid synthesis. In both areas, the fish migrated to suitable environment for spawning from habitat place just after full moon. It appears that the environment has water depth necessary to do the spawning behavior.

蜂窝石斑鱼是一种与月相关的产卵鱼，分布于太平洋亚热带和热带海域。然而，在这个物种的性腺发育和产卵的细节还没有得到很好的理解。本研究以日本冲绳的濑子岛和西表岛为研究对象，对产卵期雌性的性腺发育、激素变化和促性腺激素基因的表达进行了研究。此外，我们还利用生物遥测技术研究了产卵洄游到适宜产卵环境的过程，结果表明，在这两个地区采集的鱼类卵巢都被卵黄晚期的卵母细胞所占据，此时性腺指数增加，并在满月前后达到最大值。然而，Ireornete岛的鱼类成熟尺寸小于Sesoko岛。此外，Treomote鱼开始雌性到雄性的性别变化后，立即产卵季节。另一方面，从濑子岛收集的鱼的性别变化在产卵季节后的几个月内开始。这两个地区的不同特征可能取决于密度、礁体生态位、深度、水流和水温的差异。产卵前的雌激素水平很高。LH β亚基基因在雌性石斑鱼生殖期表达，提示LH在性腺发育和类固醇合成中具有重要作用。在这两个地区，鱼类迁移到合适的环境，从栖息地产卵后，满月。看来环境具有进行产卵行为所需的水深。