ABNORMAL FORMATION OF SEROTONERGIC NETWORK AND STRESS ACTION.

血清素网络和压力作用的异常形成。

• 批准号: 11680769
• 负责人: NISHIO Hiroaki
• 金额: $ 2.37万
• 依托单位: FUKUYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680769/
• 关键词: SOUND STRESS; NEONATAL RAT; EMONTION; LEARNING; SEX-DEPENDENT; SEROTONIN (5-HT); TRANSPORTER; RECEPTOR; 情動行動; セロトニン; トランスポーター

Maternal sound stress (800 Hz ; 77dB, every other minute for 15 min/day, from day 10 to 18 of gestation), combined with forced swimming stress (15 min/day), was found to cause potentiation of sound-induced loss of locomotor activity, referred to as emotional behavior, of male offspring, but not that of female offspring, at 4 weeks of age. Maternal stress also caused an increase in the total number of errors by male, but not female offspring in the water-maze test at 6 weeks of age. These effects of stress on emotional behavior and learning behavior were abolished when dams were pretreated with buspirone (30 min before the stress, from day 8 to 18 of gestation). Thus, prenatal stress might have sex-dependent effects on emotional behavior and learning ability of neonatal rats. As to the neurochemical aspects, maternal sound stress, combined with forced swimming stress, was found to influence the binding activity of 5-HT_<2A> receptor and 5-HT transporter to their specific ligand in sex-dependent manner. No effect was observed in the binding activity of 5-HT_<1A> receptor of developing brain. Thus, it was speculated that maternal stress might cause abnormal formation of serotonergic network, and might impair the emotional behavior and learning ability of neonatal rats.

母体声音应激（800 Hz ; 77 dB，每隔一分钟持续15分钟/天，从妊娠第10天至第18天），与强迫游泳应激（15分钟/天）相结合，发现在4周龄时，雄性后代的声音诱导的运动活动（称为情感行为）丧失增强，但雌性后代则不然。母亲的压力也导致了男性的错误总数增加，但不是女性后代在6周龄的水迷宫测试。当母鼠用丁螺环酮预处理（应激前30分钟，妊娠第8天至第18天）时，应激对情绪行为和学习行为的影响被消除。因此，产前应激可能对新生大鼠的情绪行为和学习能力产生性别依赖性影响。在神经化学方面，发现母体声应激和强迫游泳应激均能影响5-HT受体<2A>和5-HT转运体与其特异性配体的结合活性，且具有性别依赖性。对发育中脑5-HT_2受体结合活性无影响<1A>。由此推测，母体应激可能导致新生大鼠神经元网络的异常形成，并可能损害新生大鼠的情绪行为和学习能力。

项目成果

Hiroaki Nishio,Shigeo Kasuga,Mitsuyasu Ushijima & Yasuo Harada: "Prenatal stress and postnatal development of neonatal rats - sex-dependent effects on emotional behavior and learning ability of neonatal rats"International Journal of Developmental Neurosci

西尾宏明、春日茂夫、牛岛光泰

Hiroaki Nishio,Shigeo Kasuga,Mitsuyasu Ushijima & Yasuo Harada: "Prenatal stress and postnatal development of neonatal rats-sex-dependent effects on emotional behavior and learning ability of neonatal rats"International Journal of Developmental Neuroscien

西尾宏明、春日茂夫、牛岛光泰

Hiroaki Nishio, Shigeo Kasuga, Mitsuyasu Ushijima & Yasuo Harada: "Prenatal stress and postnatal development of neonatal rats - sex-dependent effects on emotional behavior and learning ability of neonatal rats"International Journal of Developmental Neuros

西尾宏明、春日茂雄、牛岛光泰