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Study on output mechanism from the superior colliculus concerning vertical saccadic eye movement.

上丘垂直扫视眼运动输出机制研究

基本信息

批准号：
11680804
负责人：
SUGIUCHI Yuriko
金额：
$ 2.37万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680804/
关键词：
vertical eye movement saccadic eye movement superior colliculus ocular motoneuron

项目摘要

(1) Anatomical identification of supranuclear gaze center of vertical eye movement.Distribution of premotor neurons projecting to motoneurons concerning vertical eye movements was investigated. In anesthetized cats with Nembutal, wheat germ agglutinin conjutated to horseradish peroxidase (WGA-HRP) was injected into the nerves of the eye muscles which concern vertical eye movements. WGA-HRP was taken up by motor axons and retorogradely transported to their cell bodies. It was further taken up by synaptic terminals of premotor neurons terminating on those motoneurons and their cell bodies were transsynaptically labeled. This study revealed that the last-order premotor neurons were located in the field of Forel, the interstitial nucleus of Cajal, and the vestibular nuclei, and the distribution of such neurons were analyzed.(2) Identification of location of output neurons of the superior colliculus which produce upward and downward eye movements.After identifiying the location in the superior colliculus from which evoke upward or downward eye movement was evoked by electrical stimulation, biocytin (anterograde tracer) was injected into the superior colliculus and projections from the superior colliculus to the brain stem structure were investigated.(3) Identification of projection from the superior colliculus to supuranuclear neurons using a double labeling technique.Injection of biocytin into the superior colliculus using the mothod mentioned in (2) and injection of WGA-HRP into the muscle nerves using the method mentioned in (1) were performed in the same animal. This study enabled us to identify the last order premotor neurons receiving input from the superior colliculus and terminating on ocular motoneurons.

(1)垂直眼动核上凝视中心的解剖鉴定。研究了运动前神经元向运动神经元投射的分布。在用Nembutal麻醉的猫中，将小麦胚芽凝集素与辣根过氧化物酶结合（WGA-HRP）注射到与眼部垂直运动有关的眼肌神经中。WGA-HRP被运动轴突摄取并逆行转运至其细胞体。它进一步被终止于这些运动神经元上的前运动神经元的突触末端吸收，它们的细胞体被跨突触标记。本研究发现，末级运动前神经元主要分布在前庭核、Cajal间质核和Forel区，并分析了这些神经元的分布。(2)识别产生上下眼动的上丘输出神经元的位置。在确定电刺激引起眼球上下运动的上丘位置后，将生物细胞素（顺行示踪剂）注入上丘，观察上丘向脑干结构的投射。(3)利用双标记技术识别上丘向核上神经元的投射。采用(2)的方法向上丘注射生物细胞素，采用(1)的方法向肌肉神经注射WGA-HRP。这一研究使我们能够确定从上丘接收输入并终止于眼运动神经元的最后一级前运动神经元。