Development of artificial skin containing biological active substance with wound healing potency

开发含有具有伤口愈合功效的生物活性物质的人造皮肤

• 批准号: 11680844
• 负责人: KUROYANAGI Yoshimitsu
• 金额: $ 2.24万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680844/
• 关键词: biological active substance; dibutyryl cyclic AMP; fibroblasts growth factor; endothelial growth factor; fibroblasts; hyaluronic acid; collagen; artificial skin; 細胞成長因子; 創傷被覆材; アスコルビン酸2リン酸; 創傷治癒

This study was designed to develop a new artificial skin containing biological active substance acceptable for the treatment of burn injury and chronic skin ulcer. The artificial skin composed of hyaluronic acid spongy containing dibutyryl cyclic AMP was proved very effective in wound healing in animal tests using rats. In addition, the authors focused on the potency of fibroblasts. These cells are able to release vascular endothelial growth factor which is necessary for promoting vasculalization. The artificial skin composed of fibroblasts combined with a two-layered spongy matrix of hyaluronic acid and collagen was proved very effective in wound healing in clinical trials. Through this study, two types of artificial skins were developed. One is an artificial skin containing biological active substance. Another is an artificial skin containing fibroblasts which are able to release biological active substances.

本研究旨在研制一种新的含有生物活性物质的人工皮肤，用于治疗烧伤和慢性皮肤溃疡。用含双丁酰环磷酸腺苷的透明质酸海绵制成的人工皮肤，在动物实验中对大鼠创面的愈合有很好的效果。此外，作者还关注了成纤维细胞的潜能。这些细胞能够释放促进血管形成所必需的血管内皮生长因子。由成纤维细胞结合透明质酸和胶原蛋白的双层海绵基质组成的人工皮肤在临床试验中被证明对伤口愈合非常有效。通过这项研究，开发了两种类型的人工皮肤。一种是含有生物活性物质的人造皮肤。另一种是含有能够释放生物活性物质的成纤维细胞的人造皮肤。

项目成果

黒柳能光: "臨床看護"ドレツッングの最前線;生体ドレッシング材と成長因子. 1698-1704 (1999)

黑柳义光：“临床护理”敷料的前沿；生物敷料和生长因子1698-1704（1999）。

Yoshimatsu Kuroyanagi: "Tissue-engineered product : allergenic cultured dermal substitute composed of spongy collagen with fibroblasts"Artificial Organs. 25. 180-186 (2001)

Yoshimatsu Kuroyanagi：“组织工程产品：由海绵状胶原蛋白和成纤维细胞组成的致敏性培养真皮替代品”人工器官。

田島道弘,井爪正人,福島貴弘,木村孝,黒柳能光: "N-サクシニル化キトサンとゼラチンからなる創傷被覆材の開発"生体材料. 18. 220-226 (2000)

Michihiro Tajima、Masato Izume、Takahiro Fukushima、Takashi Kimura、Yoshimitsu Kuroyanagi：“由 N-琥珀酰化壳聚糖和明胶组成的伤口敷料材料的开发”生物材料 18. 220-226 (2000)。

黒柳能光: "分子心血管病"再生組織工学を応用したバイオ人工皮膚の開発. 79-85 (2001)

Yoshimitsu Kuroyanagi：“分子心血管疾病”应用再生组织工程的生物人工皮肤的开发79-85（2001）。

黒柳能光: "創傷治癒と再生医療"バイオサイエンスとインダストリー. 58. 704-707 (2000)

Yoshimitsu Kuroyanagi：“伤口愈合和再生医学”《生物科学与工业》58. 704-707 (2000)。