Development of autologous cultured dermal substitute for treatment of burn scarand giant nevus

儿童严重的烧伤瘢痕会导致不良的功能障碍。儿童巨痣可引起精神障碍。本研究旨在开发自体培养真皮替代物（auto-CDS）来治疗这些皮肤病。本研究的结果证明了以下证据。1)对自体cds的低温保存进行了详细的研究。开发了一种适合于自动cds的能保持其物理性能的基体。2)低温保存和解冻后的CDS能够维持生成血管生成所必需的各种生长因子的能力，包括VEGF、HGF、bFGF。3)在开发自动光盘的同时，开发了适合自动光盘的覆盖材料。这种覆盖材料由含有表皮生长因子的透明质酸海绵状薄片组成。培养系统的结果显示，覆盖材料释放的EGF对自体cds的成纤维细胞有刺激作用，因此成纤维细胞可以释放更多的VEGF和HGF。自体cds与表皮生长因子结合的覆盖材料能够成功地治疗儿童严重烧伤疤痕和巨大痣。

Severe burn scar in children results in the undesirable functional disturbance. Giant nevus in children results in the mental disturbance. This study was aimed to develop the autologous cultured dermal substitute (auto-CDS) for the treatment of these skin diseases. The results in this study proved the following evidences. 1) The cryopreservation for auto-CDS was investigated in detail. The suitable matrix for auto-CDS that is able to keep the physical properties was developed. 2) The cryopreserved and thaued CDS is able to maintain the potency to produce various growth factors including VEGF, HGF, and bFGF that are essential for angiogenesis. 3) As well as developing auto-CDS, the proper covering material for aluto-CDS was developed. This covering material is composed of a hyaluronic acid spongy sheet containing EGF. The results in culture system revealed that EGF released from covering material functions to stimulate fibroblasts in auto-CDS, and thereby fibroblasts can release more amounts of VEGF and HGF. The use of auto-CDS in conjunction with the EGF-incorporated covering material is able to provide a successful treatment for severe burn scar and giant nevus in children.