Development of autologous cultured dermal substitute for treatment of burn scarand giant nevus

治疗烧伤疤痕及巨痣的自体培养真皮替代品的研制

• 批准号: 19500407
• 负责人: KUROYANAGI Yoshimitsu
• 金额: $ 2.75万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19500407/
• 关键词: 培養真皮; 線維芽細胞; ヒアルロン酸; コラーゲン; 熱傷瘢痕; 色素性母斑

Severe burn scar in children results in the undesirable functional disturbance. Giant nevus in children results in the mental disturbance. This study was aimed to develop the autologous cultured dermal substitute (auto-CDS) for the treatment of these skin diseases. The results in this study proved the following evidences. 1) The cryopreservation for auto-CDS was investigated in detail. The suitable matrix for auto-CDS that is able to keep the physical properties was developed. 2) The cryopreserved and thaued CDS is able to maintain the potency to produce various growth factors including VEGF, HGF, and bFGF that are essential for angiogenesis. 3) As well as developing auto-CDS, the proper covering material for aluto-CDS was developed. This covering material is composed of a hyaluronic acid spongy sheet containing EGF. The results in culture system revealed that EGF released from covering material functions to stimulate fibroblasts in auto-CDS, and thereby fibroblasts can release more amounts of VEGF and HGF. The use of auto-CDS in conjunction with the EGF-incorporated covering material is able to provide a successful treatment for severe burn scar and giant nevus in children.

儿童严重的烧伤瘢痕会导致不良的功能障碍。儿童巨痣可引起精神障碍。本研究旨在开发自体培养真皮替代物（auto-CDS）来治疗这些皮肤病。本研究的结果证明了以下证据。1)对自体cds的低温保存进行了详细的研究。开发了一种适合于自动cds的能保持其物理性能的基体。2)低温保存和解冻后的CDS能够维持生成血管生成所必需的各种生长因子的能力，包括VEGF、HGF、bFGF。3)在开发自动光盘的同时，开发了适合自动光盘的覆盖材料。这种覆盖材料由含有表皮生长因子的透明质酸海绵状薄片组成。培养系统的结果显示，覆盖材料释放的EGF对自体cds的成纤维细胞有刺激作用，因此成纤维细胞可以释放更多的VEGF和HGF。自体cds与表皮生长因子结合的覆盖材料能够成功地治疗儿童严重烧伤疤痕和巨大痣。

项目成果

A case of pyoderma gangrenosum with intractable leg ulcers treated by allogeneic cultured dermal substitutes.

同种异体培养真皮替代物治疗坏疽性脓皮病合并顽固性腿部溃疡一例。

• 发表时间: 2008
• 期刊: Dermatology Online Journal 14(11)
• 作者: Toyozawa S;Yamamoto Y;Nishide T;Kishioka A;Kanazawa N;Matsumoto Y;Kuroyanagi Y;Furukawa F: Case report
• 通讯作者: Furukawa F: Case report

Standardization for mass production of allogeneic cultured dermal substitute by measuring the amount of VEGF, bFGF, HGF, TGF-β, and IL-8.

通过测量 VEGF、bFGF、HGF、TGF-β 和 IL-8 的量，实现同种异体培养真皮替代品大规模生产的标准化。

• 发表时间: 2008
• 期刊: J. Artif. Organs 11
• 作者: Hashimoto A;Kuroyanagi Y
• 通讯作者: Kuroyanagi Y

Therapeutic angiogenesis by autlogous bone marrow cell implantation inconjunction with allogeneic cultured dermal substitute for intractable ulcers in critical limb ischemia.

通过自体骨髓细胞植入联合同种异体培养真皮替代物进行治疗性血管生成，治疗严重肢体缺血的顽固性溃疡。

• 期刊: Ann. Plast. Reconstr (in press)
• 作者: Mizuno H;Shimamoto M;Koike S;Hyakusoku H;Miyamoto M;Kuroyanagi Y
• 通讯作者: Kuroyanagi Y

アルギニン含有ピアルロン酸創傷被覆材の糖尿病マウスにおける創傷治癒促進効果

含精氨酸丙醛酸伤口敷料促进糖尿病小鼠伤口愈合的作用

• 发表时间: 2009
• 作者: 松本靖広;中野幹人;黒柳能光
• 通讯作者: 黒柳能光

患者までとどいている再生誘導治療(培養真皮)

再生诱导治疗到达患者身边（培养真皮）

• 发表时间: 2008
• 作者: 小澤信介;石川浩平;西原裕晶;伊藤逸毅;他;黒柳能光
• 通讯作者: 黒柳能光