Establishment of noble therapy using allogeneic cultured dermal substitute combined with wound dressing containing epidermal growth factor

本研究旨在探索异体皮肤替代物与含有表皮生长因子(EGF)的创面敷料相结合治疗皮肤缺损的新方法。最后，我们研制出了一种优良的双层培养真皮替代物(CDS)。上层是含有表皮生长因子(EGF)的透明质酸(HA)和胶原(Col)海绵状薄片。下层是含有成纤维细胞的HA海绵状薄片和Col凝胶。CDS很有前景，因为冷冻保存、解冻和漂洗过程没有造成细胞损伤的风险。CDS在无血清介质中制备，然后放置在伤口表面。关键问题是如何减少在无血清培养液中长时间孵育对细胞的损伤。加入EGF的CDS在无血清条件下孵育数天后即可使用。这段时间足以将CDS从制造设施运送到医院。这种CDS有望成为一种实用的治疗方法。

This study aimed to establish a novel therapy for skin defect using allogeneic cultured skin substitute combined with wound dressing containing epidermal growth factor (EGF). Finally we developed an excellent two-layered cultured dermal substitute (CDS). The upper layer is a hyaluronic acid (HA) and collagen (Col) spongy sheet with epidermal growth factor (EGF). The lower layer is a HA spongy sheet and Col gel containing fibroblasts. The CDS is promising, because there is no risk of cellular damage caused by cryopreservation, thawing and rinsing processes. The CDS is prepared in serum-free medium, followed by placing on a wound surface. The critical issue is how to reduce the cellular damage during a prolonged period of incubation in serum-free medium. EGF-incorporating CDS can be used after a period of several days incubation in serum-free medium. This period is sufficient for transport of CDS from manufacturing facilities to hospitals. This CDS is promising for a practical therapy.