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Establishment of noble therapy using allogeneic cultured dermal substitute combined with wound dressing containing epidermal growth factor

同种异体培养真皮替代品联合含表皮生长因子伤口敷料建立贵族疗法

基本信息

批准号：
23500545
负责人：
KUROYANAGI Yoshimitsu
金额：
$ 3.33万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

项目摘要

This study aimed to establish a novel therapy for skin defect using allogeneic cultured skin substitute combined with wound dressing containing epidermal growth factor (EGF). Finally we developed an excellent two-layered cultured dermal substitute (CDS). The upper layer is a hyaluronic acid (HA) and collagen (Col) spongy sheet with epidermal growth factor (EGF). The lower layer is a HA spongy sheet and Col gel containing fibroblasts. The CDS is promising, because there is no risk of cellular damage caused by cryopreservation, thawing and rinsing processes. The CDS is prepared in serum-free medium, followed by placing on a wound surface. The critical issue is how to reduce the cellular damage during a prolonged period of incubation in serum-free medium. EGF-incorporating CDS can be used after a period of several days incubation in serum-free medium. This period is sufficient for transport of CDS from manufacturing facilities to hospitals. This CDS is promising for a practical therapy.

本研究的目的是建立一种新的治疗皮肤缺损的方法，使用同种异体培养皮肤替代物结合创面敷料含有表皮生长因子（EGF）。最后研制出一种性能优良的双层培养真皮替代物。上层是透明质酸（HA）和胶原蛋白（Col）海绵片与表皮生长因子（EGF）。下层是含有成纤维细胞的HA海绵片和Col凝胶。CDS是有前途的，因为没有由冷冻保存、解冻和冲洗过程引起的细胞损伤的风险。CDS在无血清培养基中制备，然后置于伤口表面上。关键的问题是如何减少在无血清培养基中长时间孵育期间的细胞损伤。在无血清培养基中孵育几天后，可以使用EGF掺入的CDS。这段时间足以将CDS从生产设施运输到医院。这种CDS有望成为一种实用的治疗方法。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Wound dressing composed of hyaluronic acid and collagen containing EGF or bFGF: comparative culture study

DOI：
10.1080/09205063.2012.731375
发表时间：
2013-05
期刊：
Journal of Biomaterials Science, Polymer Edition
影响因子：
0
作者：
Akane Yu;Hayato Niiyama;Shinya Kondo;A. Yamamoto;Ryusuke Suzuki;Y. Kuroyanagi
通讯作者：
Akane Yu;Hayato Niiyama;Shinya Kondo;A. Yamamoto;Ryusuke Suzuki;Y. Kuroyanagi

Effect of EGF and bFGF on fibroblast proliferation and angiogenic cytokine production from cultured dermal substitute.

EGF 和 bFGF 对培养真皮替代物中成纤维细胞增殖和血管生成细胞因子产生的影响。