Investigation on Insect-Microbial Symbiosis and Molecular Phylogeny of Social Insects

社会昆虫昆虫-微生物共生及分子系统发育研究

• 批准号: 11691174
• 负责人: MATSUMOTO Tadao
• 金额: $ 11.8万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11691174/
• 关键词: Brazil; molecular phylogeny; Guatemala; Neo-tropical region; termites; aphids; Passalidae; symbiosis; メキシコ; グアテマラ; フランス; テングシロアリ; ミトコンドリアDNA; 社会性昆虫; 共生微生物

The investigated sites were selected at the highly natural places in Brazil, Mexico, Guatemala of the Neo-tropical region. The investigated materials were termites, ants, aphids in social insects, subsocial Passalidae and cockroaches. The symbiotic relationships between the insects and microorganisms (bacteria, fungi and protozoa) were investigated. Collections of insects were performed in wide area as possible as done. And investigated places were selected along humidity cline from humid to dry regions. Research discussions on the relationship of insects and microorganisms were carried out in Renne(France), in New York (USA) and in Iguasu (Brazil). Detailed observations on insect samples by a light microscope and an electro microscope were done. Phylogenetic studies were performed by the analysis of mtDNA in insects and microorganisms. The results were compared to the already known informations in the Nansei-Archipelago and Taiwan of the Palaeoarctic region.

调查地点选择在新热带地区的巴西、墨西哥、危地马拉等自然条件较好的地方。调查对象为白蚁、蚂蚁、社会性昆虫中的蚜虫、亚社会性雀蛉科和蟑螂。研究了昆虫与微生物（细菌、真菌和原生动物）的共生关系。在尽可能大的范围内进行昆虫采集。调查地点沿着湿度梯度由湿润区向干燥区选择。在雷恩（法国）、纽约（美国）和伊瓜苏（巴西）进行了关于昆虫和微生物关系的研究讨论。用光学显微镜和电子显微镜对昆虫样品进行了详细的观察。通过分析昆虫和微生物的线粒体DNA进行系统发育研究。并与古北极南西群岛和台湾地区的已知资料进行了比较。

项目成果

Garcia J., Maekawa K., Miura T., Matsumoto T.: "Population Structure and Genetic Diversity in Insular Populations of Nasutitermes takasagoensis (Isoptera: Termiitidae) Analyzed By AFLP markers"Zoological Science. vol.19(in press). (2002)

Garcia J.、Maekawa K.、Miura T.、Matsumoto T.：“通过 AFLP 标记分析高砂白蚁岛群的种群结构和遗传多样性（等翅目：白蚁科）”动物学科学。

Miura T., Roisin Y., Matsumoto T.: "Molecular Phyogeny and Biogeography of the Nasute Terrmite Genus Nasutitermes (Isoptera: Termitidae) in the Pacific Tropics"Mol. Phylogen. Evolution. vol.17. 1-10 (2001)

Miura T.、Roisin Y.、Matsumoto T.：“太平洋热带地区 Nasute 白蚁属 Nasutitetermes（等翅目：白蚁科）的分子系统发育和生物地理学”Mol。

Maeyama,T.: "Molecular phylogeny of Rubiaceous epiphytic myrmecop phyte based on the atpB-rbcL intergene region"Tropics. 10(in press). (2001)

Maeyama，T.：“基于 atpB-rbcL 基因间区域的红花附生 myrmecop 植物的分子系统发育”热带。

Miura,T: "Soldier morphogenesis in a nasute termite : discovery of a disk-like structure forming a soldier nasus."Proc.R.Soc.Lond.B. 267. 1185-1189 (2000)

Miura,T：“鼻白蚁的士兵形态发生：发现形成士兵鼻的盘状结构。”Proc.R.Soc.Lond.B.

Miura T., Roisin Y., Matsumoto T.: "Molecular Phylogeny and Biogeography of the Nasute Termite Genus Nasutitermes (Isoptera : Termitidae) in the Pacific"Molecular Phylogenetics Evolution. 17. 1-10 (2001)

Miura T.、Roisin Y.、Matsumoto T.：“太平洋 Nasute 白蚁属 Nasutitetermes（等翅目：白蚁科）的分子系统发育和生物地理学”分子系统发育进化。