Kin structure of colony in polydoumous termites and ants

多穴白蚁和蚂蚁群体的亲缘结构

• 批准号: 07454211
• 负责人: MATSUMOTO Tadao
• 金额: $ 4.54万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07454211/
• 关键词: Nastitermes; DNA fingerprint; Formica japonica; polygynous; kin relationship; Hodotermopsis japonica; kin structure

Field surveys on Nasutitermes takasagoensis in Iriomote and Ishigaki Isl. were carried out during the three years. DNA fingerprint method was applied on the investigation of genetic polymorphism of the colonies. Increasing of relatedness between the nearest colonies were revealed. In the case of Formica japonica, queen number, mating frequency and nest kin structure of the ant were studied in the field and thelaboratory. Nest excavation in the study site, the east slope of Mt.Fuji Gotenba, Japan, revealed that F.japonica is weakly polygynous all year round and the queen number increases after the nuptial filght season, suggestinq the adoption of newly mated queens by established nests. Dissection and laboratory rearing demonstrated that nearly all queens in polygynous nests had mated and were fertile with mature oocytes in their ovaries. Multilocus DNA finqerprinting was used to examine kin relationships among ants found in the same nests. The fingerprint band patterns were apparently governed by a simple genetic rule and suggested monoandry (single mating per queeen) . The mean band sharing score of DNA fingerprints among full sisters was 0.90, and the mean value between queens and their daughters was 0.75. Comparison of DNA fingerprints of adult and pupal workers with pupal gynes suggested that multiple queens in a nest may contibute unequally to gyne (new queen) production. Hodotermopsis Japonica was also investigated on kin structure of the colonies in Yakushima Isl.

西表岛和石垣岛高砂白蚁的野外调查。在这三年中进行了。应用DNA指纹技术对群体遗传多态性进行了研究。最近的群体之间的相关性增加。以日本蚁为例，在野外和室内对蚁后数量、交配频率和巢亲结构进行了研究。在日本富士御殿场东坡进行的蚁巢发掘表明，日本赤眼蜂全年弱一夫多妻，在婚期过后，蜂王数量增加，不利于已建巢对新交配蜂王的采纳。解剖和实验室饲养表明，几乎所有的女王在一夫多妻制巢交配，并与成熟的卵母细胞在他们的卵巢生育。多位点DNA指纹被用来研究在同一个巢穴中发现的蚂蚁之间的亲缘关系。指纹带模式显然是由一个简单的遗传规律，并建议单雄交配（单交配每女王）。全姐妹间DNA指纹谱带共享度平均值为0.90，蜂王与其女儿间平均值为0.75。成虫和蛹工蜂与蛹雌蜂的DNA指纹比较表明，一个巢中的多个蜂王可能对雌蜂（新蜂王）的生产做出不平等的贡献。同时对屋久岛日本热白蚁的亲缘结构进行了研究。

项目成果

Miura T.& Matsumoto T.: "Diet and nest material of the processional termite Hospitalitermes and cohabitation of Termes(Isoptera:Termitidae)" Insectes Sociaux. 44. 267-276 (1997)

三浦T.

Miura,T & Matsumoto T.: "Foraging organization of the open-air processional lichen-feeding termite Hospitalitermes (Isoptera,Termitidae)in Borneo." Insectes Sociaux. 44. 301-316 (1997)

三浦,T

Miura T.& Matsumtoto T.: "Ergatoid preproductives in Nasutitermes takasagoensis(Isoptera:Termitidae)." Sociobiology. 27. 223-238 (1997)

三浦T.

Sato T., Masuko Kand Matsumoto, T.: "Colony gentic structure in the mono-and polygynous sibling species of the ants Camponotus nawai and Camponotus yamaokai DNA fingerprint analysis." Ecological Research. 12. 71-76 (1997)

Sato T.、Masuko Kand Matsumoto, T.：“Camponotus nawai 和 Camponotus yamaokai 的单性和多性同胞物种中的群体遗传结构分析。”

Miura, T.and Matsumoto, T.: "Diet and nest material of the processional termite Hospitalitermes, and cohabitation of Termes (Isoptera : Termitidae) on Borneo Island." Insects Sociaux. 44. 267-275 (1997)

Miura, T. 和 Matsumoto, T.：“婆罗洲岛上游行白蚁医院白蚁的饮食和巢穴材料，以及白蚁（等翅目：白蚁科）的共居。”