Regulation of Mast Cell Differentiation by mi Transcription Factor (MITE)
mi 转录因子 (MITE) 对肥大细胞分化的调节
基本信息
- 批准号:12002010
- 负责人:
- 金额:$ 124.93万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Specially Promoted Research
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We investigated the effect of MITF on development of mast cells. 1).Many mutant alleles have been reported in the mi locus that encodes MITF. We examined the interrelationship between the structure of mutant MITF and the biological effect of the mutated MITF. The mi is the firstly found mutant allele, and an arginine was deleted at the basic domain. The MITF produced by the mi mutant allele (mi-MITF) lacks DNA binding potential. Although the mi-MITF has no transcription ability, it can interact with other transcription factors and inhibit their functions (inhibitory mutation). The tg is a transgene insertion mutation, and MITF is not produced in cultured mast cells (CMCs) derived from tg/tg mice (null mutation). The phenotype of tg/tg CMCs was more severe than that of mi/mi CMCs. 2).The necessity of MITF for development of mast cells was examined by grafting bone marrow cells of normal (+/+) or tg/tg mice to irradiated W/Wv mice. W/Wv mice are deficient in mast cells due to the defect of multipotential hematopoietic stem cells. Erythrocytes, neutrophils, macrophages, B cells and T cells became of donor origin after the bone marrow transplantation from either +/+ or tg/tg mice. Mast cells developed in all examined tissues of W/Wv mice when +/+ bone marrow cells were injected, but did not when tg/tg bone marrow cells were injected. MITF appears essential for differentiation of mast cells from their precursors in tissues of adult mice.
我们研究了MITF对肥大细胞发育的影响。1).在编码MITF的mi基因座中已经报道了许多突变等位基因。我们研究了突变的MITF的结构和突变的MITF的生物学效应之间的相互关系。mi是第一个发现的突变等位基因,在碱基结构域缺失了一个精氨酸。由mi突变等位基因产生的MITF(mi-MITF)缺乏DNA结合潜力。虽然mi-MITF没有转录能力,但它可以与其他转录因子相互作用并抑制它们的功能(抑制性突变)。tg是一种转基因插入突变,并且在来自tg/tg小鼠的培养肥大细胞(CMC)中不产生MITF(无效突变)。tg/tg型细胞的表型较mi/mi型细胞严重。2).通过将正常(+/+)或tg/tg小鼠的骨髓细胞移植到经辐射的W/Wv小鼠中来检查MITF对于肥大细胞发育的必要性。W/Wv小鼠由于多能造血干细胞的缺陷而导致肥大细胞缺陷。从+/+或tg/tg小鼠骨髓移植后,红细胞、中性粒细胞、巨噬细胞、B细胞和T细胞成为供体来源。当注射+/+骨髓细胞时,肥大细胞在W/Wv小鼠的所有检查组织中发育,但当注射tg/tg骨髓细胞时,肥大细胞没有发育。MITF似乎是成年小鼠组织中肥大细胞从其前体分化所必需的。
项目成果
期刊论文数量(46)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Morii E, Oboki K, Kataoka TR, Igarashi K, Kitamura Y: "Interaction and cooperation of MITF and MAZR for transcription of mouse mast cell protease 6 gene."Journal of Biological Chemistry. 277. 8566-8571 (2002)
Morii E、Oboki K、Kataoka TR、Igarashi K、Kitamura Y:“MITF 和 MAZR 在小鼠肥大细胞蛋白酶 6 基因转录中的相互作用与合作。”生物化学杂志。
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- 影响因子:0
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Ito A, Jippo T, Wakayama Y, Morii E, Koma Y, Onda H, Nojima H, Iseki S, Kitamura Y: "SgIGSF : a new mast-cell adhesion molecule used for attachment to fibroblasts and transcriptionally regulated by MITF."Blood. 101. 2601-2608 (2003)
Ito A、Jippo T、Wakayama Y、Morii E、Koma Y、Onda H、Nojima H、Iseki S、Kitamura Y:“SgIGSF:一种新的肥大细胞粘附分子,用于附着到成纤维细胞并受 MITF 转录调节。”血液
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- 影响因子:0
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Hirota S, Kitamura Y et al.: "Familiar gastrointestinal stromal tumors associated with dysphagia and novel type germline mutation of KIT gene"Gastroenterology. 122. 1493-1499 (2002)
Hirota S、Kitamura Y 等人:“与吞咽困难相关的熟悉胃肠道间质瘤和 KIT 基因的新型种系突变”胃肠病学。
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- 影响因子:0
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Ito A, Kitamura Y et al.: "Inhibitory effect of natural killer activity of mi-MITF"Blood. 97. 2075-2083 (2001)
Ito A、Kitamura Y 等人:“mi-MITF 自然杀伤活性的抑制作用”血液。
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- 影响因子:0
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Jipppo T, Kitamura Y et al.: "Tissue-dependent alteration of protease expression phenotype in murine mast cells"American Journal of Pathology. 158. 1695-1901 (2001)
Jipppo T、Kitamura Y 等人:“小鼠肥大细胞中蛋白酶表达表型的组织依赖性改变”美国病理学杂志。
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KITAMURA Yukihiko其他文献
KITAMURA Yukihiko的其他文献
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{{ truncateString('KITAMURA Yukihiko', 18)}}的其他基金
Analysis of Phenotypic Variation of Mast Cells by Using Messenger RNA Expression as a Marker
以信使 RNA 表达为标志物分析肥大细胞表型变异
- 批准号:
06454193 - 财政年份:1994
- 资助金额:
$ 124.93万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Regulation of Hemopoietic Stem Cells and Its Clinical Application
造血干细胞的调控及其临床应用
- 批准号:
06277103 - 财政年份:1994
- 资助金额:
$ 124.93万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Development of Mutant Rats Useful for Analysis of Immediate Hypersensitivity
可用于速发型超敏反应分析的突变大鼠的培育
- 批准号:
03558010 - 财政年份:1991
- 资助金额:
$ 124.93万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Fibroblast-dependent Proliferation of Mast Cells
肥大细胞成纤维细胞依赖性增殖
- 批准号:
02404030 - 财政年份:1990
- 资助金额:
$ 124.93万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
Developemtnt, Prfuction, and promotion of usilization of Mast Cell Deficient W/W/^v and Sl/Sl^d Mice.
肥大细胞缺陷型 W/W/^v 和 Sl/Sl^d 小鼠的发育、生产和利用促进。
- 批准号:
60880010 - 财政年份:1985
- 资助金额:
$ 124.93万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research
相似国自然基金
基于CRF-Mast cell信号通路探索疏肝健脾法治疗IBS内脏高敏感的机制研究
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