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Developemtnt, Prfuction, and promotion of usilization of Mast Cell Deficient W/W/^v and Sl/Sl^d Mice.

肥大细胞缺陷型 W/W/^v 和 Sl/Sl^d 小鼠的发育、生产和利用促进。

基本信息

批准号：
60880010
负责人：
KITAMURA Yukihiko
金额：
$ 8.64万
依托单位：
Osaka University(Medical School)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

Genetically mast cell-deficient W/W^v and Sl/Sl^d mice are useful for the analysis of mast cell biology. Especially, W/W^v mine are useful as recipients of bone marrow cells and skin pieces. Inasmuch as suspentsion and clonal cultures of mast cells have been developed, wwe combined these in vivio and in vitro systems.1) Suspension-cultured mast cells had morphological and biochemical characteristics similar to those of mucosla mast cells (MMX). However, i.p. injection of such cultured mast cells bage rise to development of cells charactersitics similar to those of nonnective tissue mast cells (CTMC).2) Hwen peritoneal cells of normal +/+ mice were cultured in methylcellulose, pure mast cell colonies appeared. Cells from individual mast cell colonies were divided and injected into the skin and stomach W/W^v mice; CTMC developed in the skin and MMC in the stomach mucosa. This indicates the presence of a common precursor for CTMC and MMC.3) Morphology of such bipotent mast cell precustors was studied by using micromanipultaion About 4% of morphologically identifiable peritineal mast cells may function as the bipotent precursors,4) Although W/W^v mice showed a defect in resistance against ixodid ticks, injection of suspension-cultured mast cells normalized the defect. The examples mentioned above indicate that combinations if in vivo and in vitro systems increara the usedfulness of W/W^v mice.

遗传性肥大细胞缺陷的W/W^v和Sl/Sl^d小鼠可用于肥大细胞生物学分析。特别是，W/W/V矿物质可用作骨髓细胞和皮肤片的受体。由于肥大细胞的悬浮培养和克隆培养已经发展成熟，我们将它们在体内和体外系统中结合起来。1）悬浮培养的肥大细胞具有与粘膜肥大细胞（MMX）相似的形态学和生化特征。2）将正常+/+小鼠腹腔细胞培养于甲基纤维素中，可形成纯肥大细胞集落。将来自单个肥大细胞集落的细胞分裂并注射到W/W^v小鼠的皮肤和胃中; CTMC在皮肤中发育，MMC在胃粘膜中发育。这表明CTMC和MMC的共同前体的存在。3）通过显微操作研究这种双能肥大细胞前体的形态。大约4%的形态可鉴定的腹膜肥大细胞可能起双能前体的作用。4）尽管W/W_v小鼠对硬蜱的抵抗力存在缺陷，但注射悬浮培养的肥大细胞使缺陷正常化。上述实施例表明，体内和体外系统的组合增加了W/W +/-小鼠的可用性。