昆虫微小脳の視覚情報高次処理・統合に関する組織学的解析

昆虫微脑视觉信息高阶处理和整合的组织学分析

• 批准号: 12048223
• 负责人: 下東 美樹
• 金额: $ 2.62万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12048223/
• 关键词: 昆虫; 視覚情報; 脳内投射; Golgi染色法; Bodian染色法; 視葉; 視小葉; 前大脳; 準超薄連続切片法

昨年の研究結果に基づき,視葉から中脳への視覚ニューロンの投射をさらに詳しく調べた.2種類の蛍光色素で視葉の部域の染め分けを行った結果,視葉の最終ニューロピルlobulaから前大脳前側葉への投射様式を明らかにすることができた.それによると,lobulaの単位構造の配置は維持されたまま,軸索は交叉することなく前大脳前側葉にはいっていた.したがって,前大脳前側葉には,視葉の受容野に相当する単位構造があることが推測された.モンシロチョウの脳について,Bodian染色連続切片,岩崎法準超薄連続切片像を解析し,脳内ニューロピルの立体配置,ニューロピル間の連絡を明らかにした.とくに,視葉ニューロンが終末する部域とそれらから,記憶中枢キノコ体,運動出力センター中心複合体を結ぶ経路を同定し,Golgi標本の精査によりそれらを結ぶニューロンの形態を明らかにした.さらに,中心複合体の単位構造を構成するニューロンの形態をGolgi染色標本で精査し,それら細胞の細胞体が分布する領域をBodian染色連続切片との照合により調べた.その結果,中心複合体を構成する細胞群は中心複合体の前方と後方に位置し,後方の細胞群は中心体のFanshaped Bodyの単位構造を形成し,前方に位置するニューロンはFanshaped Bodyの全域にわたって側枝を出すことがわかった.これらの知見により,ほかの部域の脳内ニューロピルとの情報伝達様式をさらに詳しく検索する足がかりができた.

The results of last year's study are based on the results of last year's study. 2. The results of last year's study show that there are significant differences in the results of last year's study. The results of last year's study show that the results of last year's study are based on the results of last year's study. 2. The results of last year's study show that the results of last year's research are very important. In order to improve the performance of the system, the lobula configuration is used to maintain the configuration, and the cable crossover is required to make sure that you are in the front of the building. In the front of the building, there is a lot of room for tolerance in the front of the building. The ultrathin section of the Iwasaki method is like "parsing", the internal configuration is different, and the link is clearly linked to each other. In the end of the day, you will be able to record the data of the central computer, the copy of the center, the copy of the center, and the Golgi tag. The results of the system will be the same as those of the central complex. In this paper, the location of the central complex is made into the shape of the Golgi staining, the distribution of the cell body, the field of the Bodian staining, the section of the section, and the shape of the cell. According to the results of the experiment, the central complex was formed into the front and rear position of the central complex, the Fanshaped Body position of the central body of the cell population, and the position in front of the cell population, the central body of the cell population, the center of the cell population, the center of the cell population, the central body of I don't know if I know what I'm talking about. I don't know. I don't know.

项目成果

Meinertzhagen I.A.& Shimohigashi M. et al: "Photoreceptor phenotypes for Drosophila mutants nonC and hdcjk910."Bull.Can.Soc.Zool.. 31(2). 7 (2000)

迈纳茨哈根 I.A.

Tomioka K.& Shimohigashi M. et al: "Immunohistochemical demonstration of Gryllus PDF,a bioactive peptide isolated from the brain of the cricket Gryllus bimaculatus."Comp.Biochem.Physiol.. 127. 374 (2000)

富冈 K.

Shimohigashi M. et al.: "Molecular cloning and characteristic expression profile of clock protein PERIOD in silk moth Bombyx mori"Proceeding for 6th International Congress of Neuroethology. 44 (2001)

Shimohigashi M.等人：“丝蛾Bombyx mori中时钟蛋白PERIOD的分子克隆和特征表达谱”第六届国际神经行为学大会论文集。

Kinoshita M., Shimohigashi M.et al.: "Projection sites of the optic lobe outputs in the brain of the swallowtail butterfly Papilio xuthus"Zoological Science. 18(Supplement). 101 (2001)

Kinoshita M.、Shimohigashi M.等人：“燕尾蝴蝶 Papilio xuthus 大脑中视叶输出的投影位点”动物学。

Toda K., Shimohigashi M.et al.: "Mapping of the butterfly brain : Identification of major neuropiles and descending neuron clusters in the brain of the swallowtail butterfly Papilio xuthus"Zoological Science. 18(Supplement). 101 (2001)

Toda K.、Shimohigashi M.等人：“蝴蝶大脑的绘图：燕尾蝴蝶 Papilio xuthus 大脑中主要神经桩和下降神经元簇的识别”动物学。