Polymer Science from Single Chains Investigated by Scanning Photon Microscopy-Non-statistic Observation of Morphology and Molecular Assembly

本研究计画系利用最新发展之显微镜，扫描光子显微镜，对聚合物进行奈米级之研究。这种新型的显微镜使我们能够观察单链的高分辨率超过光波的衍射限制，和作为光学显微镜的上级性能已被应用于研究单链，分子组装，界面和聚合物材料的单层的形态。迄今为止，这些研究都是在整体平均的基础上进行的，即使是分子水平的讨论，但本项目致力于从单个链的真实的图像发展非平均、非统计的聚合物科学和技术。主要问题如下：1）通过扫描光子显微镜观察嵌入单分子膜中的单个大分子的二维构象。2）表面吸附，刚性DNA链在固体基质上的链形态和取向; 3）通过荧光显微镜和能量转移动力学研究互穿聚合物网络的非均匀纳米结构; 4）聚合物共混物单分子膜的二维相分离; 5）深紫外扫描光子显微镜的研制及“无荧光”聚合物样品的观察。

This research project is concerned with nanometric investigation of polymers by means of recently developed microscopy, scanning photon microscopy. This new type microscopy enables us to observe single chains with a high resolution over the diffractive limitation of light wave, and the superior performance as an optical microscope has been applied for investigating morphology of single chains, molecular assemblies, interfaces, and monolayers of polymeric materials. So far these researches have been performed on the basis of quantities ensemble-averaged even for the molecular level discussions, but this project is devoted to develop non-averaged, non-statistic polymer science and technology from real image of individual chains. The major issues are listed below.1)Two-dimensional conformation of a single macromolecule embedded in monolayers observed by scanning photon microscopy.2)Surface adsorption, chain morphology and orientation of rigid DNA chains on a solid substrate.3)Inhomogeneous nanometric structure of interpenetrating polymer network studied by fluorescence microscopy and energy transfer kinetics.4)Two-dimensional phase separation of polymer blend monolayers.5)Development of deep UV scanning photon microscope and observation of "non-fluorescnent" polymer samples.