Studies on Polymer Structures and Functions in Nanometric Dimensions: Challenges to Ten Nano-meters Regions by Scanning Photon Microscopy

纳米尺寸聚合物结构与功能研究：扫描光子显微镜挑战十纳米区域

基本信息

批准号：
15205016
负责人：
ITO Shinzaburo
金额：
$ 32.86万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

This research project is aimed at development of a novel scanning photon microscope characterized both with high spatial resolutions of 10-100 nm and with high sensitivities capable of detecting extremely weak emission from a single molecule. Owing to the excellent performance, this research could exemplify the usefulness of this novel method for studying polymer structures and functions in the "critical dimensions" between microscopic molecular systems and macroscopic bulk systems. The issues investigated during the project term are listed as follows;1. Real space imaging of conformation and expansions of single polymer chains in a polymer matrix2. Polymer structure and entanglement in a restricted space such as ultra-thin polymer films and surfaces3. Quantitative evaluation of spatial distribution of near-field optical waves and spatial resolution of microscope by using model samples prepared with highly fluorescent quantum dots4. Analysis of molecular diffusion at the polymer surface and interfaces in a nanometric dimensions5. Study of segmental orientation of main-chain conjugated polymers by fluorescence polarization imaging with a dual detection scanning photon microscope6. Polymer chain orientation under mechanical elongation of polymer films; comparative study of macroscopic stress-strain and molecular level orientation7. Development of a new scanning photon microscope working for a liquid environment specimen, and high resolution observation of biomaterials.

该研究项目旨在开发一种新型的扫描光子显微镜，该显微镜具有高空间分辨率为10-100 nm，并且具有高灵敏度，能够检测到单个分子的极弱发射。由于表现出色，这项研究可以体现这种新型方法在研究微观分子系统和宏观体积系统之间“临界维度”中的聚合物结构和功能的实用性。项目术语期间研究的问题如下列出； 1。聚合物矩阵中单个聚合物链的构象和膨胀的真实空间成像。聚合物结构和纠缠在受限空间中，例如超薄聚合物膜和表面3。使用用高荧光量子点制备的模型样品4的定量评估近场光波和显微镜空间分辨率的定量评估。分析在聚合物表面和纳米尺寸中的界面上的分子扩散5。通过双重检测扫描光子显微镜的荧光极化成像对主链共轭聚合物的分段取向的研究。在聚合物膜的机械伸长下聚合物链方向；宏观应力 - 应变和分子水平方向的比较研究7。为液体环境标本工作的新型扫描光子显微镜的开发以及生物材料的高分辨率观察。