Development of bovine milk antibody to prevent dental caries

由致龋变形链球菌产生的细胞表面蛋白抗原（PAc）和水不溶性葡聚糖合成酶（GTF-I）是该细菌在人类口腔中定植的两个主要因素。我们研究了牛奶的效果，免疫后产生的这些蛋白质的功能结构域的融合蛋白，对变形链球菌的pesticonizaion。将PAc的唾液结合丙氨酸富集区（PAcA）与GTF-Ⅰ的葡聚糖结合区（GB）融合，制备PAcA-GB融合蛋白，免疫荷斯坦奶牛，获得免疫乳。8名成年受试者接受西吡氯铵（CPC）治疗后，一个亚组（n=4）用免疫牛奶漱口，对照组（n=4）用非免疫牛奶漱口。CPC治疗后，两组唾液和牙菌斑中的变形链球菌水平均下降。免疫乳漱口能显著抑制唾液和菌斑中变形链球菌的增殖。另一方面，N ...更多信息 对照组唾液和菌斑中变形链球菌数量在CPC治疗后立即增加，并分别在CPC治疗后42和28天超过基线水平。免疫乳组唾液和菌斑中变形链球菌占链球菌总数的比例低于对照组。这些结果表明，从免疫奶牛生产的牛奶可能是有用的控制变形链球菌在人口腔中。我们研究了免疫牛奶的影响，变形链球菌在大鼠模型中的致龋性。用浓缩免疫乳喂养大鼠，每日1次，连续55天。牛乳铁蛋白分子和含有残基473 - 538的相对长的乳铁蛋白片段强烈抑制变形链球菌对唾液包被的羟基磷灰石珠的粘附。Lf 411中的每个半胱氨酸残基（居民473至538）被丝氨酸残基取代，并且突变体Lf 411-C481 S和Lf 411-C532 S强烈抑制变形链球菌粘附。这些结果表明，结合唾液膜的乳铁蛋白的功能结构域位于残基473至538，并且该区域可能被Lf 411片段中Cys 481和Cys 532之间的二硫键形成所隐藏。少

Cell surface Protein antigen(PAc) and water-insoluble glucan-synthesizing enzyme(GTF-I) produced by cariogenic Streptococcus mutans are two major factors implicated in the colonization of the human oral cavity by this bacterium. We examined the effect of bovine milk, produced after immunization with a fusion protein of functional domains of these proteins, on the recolonizaion of S.mutans. To prerare immune milk, a pregnant Holstein cow was immunized with the fusion protein PAcA-GB, a fusion of the saliva-binding alanine-rich region(PAcA) of PAc and the glucan-binding(GB) domain of GTF-I. After eight adult subjects received cetylpyridinium chloride(CPC) treatment, one subgroup(n=4) rinsed their mouths with immune milk arid a control group(n=4) rinsed with nonimmune milk. S.mutans levels in saliva and dental plaque decreased after CPC treatment in both groups. Mouth rinsing with immune milk significantly inhibited recolonization of S.mutans in saliva and plaque. On the other hand, the n … More umber of S.mutans cells in saliva and plaque in the control group increased immediately after the CPC treatment and surpassed the baseline level 42 and 28 days, respectively, after the CPC treatment. The ratios of S.mutans to total streptococci in saliva and plaque in the group that received immune milk were lower than those in the control group. These results suggest that milk produced from immunized cow may be useful for controlling S.mutans in the human oral cavity.We examined the effect of the immune milk on the cariogenicity of S.mutans in a rat model. Concentrated immune milk was fed to rats once a day for 55 days. The group that received immune milk had significantly less caries development than controls.The bovine lactoferrin molecule and relatively long lactoferrin fragments containing residues 473 to 538 strongly inhibited adherence of S.mutans to saliva-coated hydroxyapatite beads. Each cysteine residue in Lf411(residents 473 to 538) was replaced by a serine residue, and the mutants Lf411-C481S and Lf411-C532S strongly inhibited S.mutans adherence. These results suggests that the functional domain of lactoferrin that binds to a salivary film lies in residues 473 to 538 and that region might be concealed by disulfide bond formation between Cys481 and Cys532 in the Lf411 fragment. Less