Identification and Determination of Unstable Chemical Species Induced by the Solar Radiation in Natural Waters.

天然水中太阳辐射引起的不稳定化学物质的鉴定和测定。

• 批准号: 12640590
• 负责人: KITAO Fujiwara
• 金额: $ 2.69万
• 依托单位: Tokyo University of Pharmacy and Life Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640590/
• 关键词: solar radiation; photochemical reaction; active oxygen; natural water; seawater; hydrogen peroxide; superoxide negative; humic acid; 活性酸素; NADH; フローインジェクション法; ニトロブルーテトラゾリウム; 海洋表層; ポルフィクン錯体; 水和電子

It is considered that the active oxygen species generate in the surface of natural waters under irradiation of the sun. In coastal seawater which contains abundant organic substance, hydrogen peroxide, one of the major active oxygen, generates up to 300 nM by the solar irradiation. OH radical, superoxide negative ion, singlet oxygen are the active oxygen species other than hydrogen peroxide. However, these species have much shorter life time compared with hydrogen peroxide, and difficult to determine. We investigated the methods to determine OH radical and superoxide negative ion in river and seawater after irradiation of solar simulator. OH radical is detected by means of conversion rate of benzene to phenol and separated by HPLC. In the case of superoxide negative ion, lactate dehydrogenase-NADH reaction, nitroblue-tetrazolium method, and nitromethane method were investigated. However, deionized water produce more superoxide negative ion than river water after the irradiation of solar simulator. Flow injection type chemiluminescence detector was constructed and chemiluminescence was detected after alkaline luminol and superoxide negative ion. In this case, humic acid gave a strong chemiluminescence compared with deionized water after irradiation of solar simulator.

认为天然沃茨表面在太阳照射下产生活性氧。在含有丰富有机物的近海海水中，过氧化氢是主要的活性氧之一，在太阳照射下产生高达300 nM的过氧化氢。OH自由基、超氧阴离子、单线态氧是过氧化氢以外的活性氧物种。然而，与过氧化氢相比，这些物种的寿命要短得多，并且难以测定。研究了太阳模拟器照射后河水和海水中OH自由基和超氧阴离子的测定方法。OH自由基的检测采用苯转化为苯酚的转化率法，分离采用高效液相色谱法。在超氧阴离子的情况下，研究了乳酸脱氢酶-NADH反应、硝基蓝-四氮唑法和硝基甲烷法。而去离子水经太阳模拟器照射后产生的超氧阴离子比河水多。构建了流动注射化学发光检测器，并对碱性鲁米诺和超氧阴离子进行了化学发光检测。在这种情况下，腐殖酸给出了一个强的化学发光与去离子水相比，太阳模拟器照射后。

项目成果

Teruaki Sakurai et al.: "Modulation of cell sdhesion and viability of cultured murine bone marow cells by arsenobetaine, a major organic compound in marine animals"Br.J.Pharmacol. 132. 143-150 (2001)

Teruaki Sakurai 等人：“砷甜菜碱（海洋动物中的一种主要有机化合物）对培养的小鼠骨髓细胞的细胞粘附和活力的调节”Br.J.Pharmacol。

藤原祺多夫: "新い検出昧よる海洋化学成分の解明"海洋化学研究. 13. 60-75 (2000)

Keitao Fujiwara：“通过新检测阐明海洋化学成分”海洋化学研究 13. 60-75 (2000)。

Tarek Samir Awad 他: "Generation of diborane from boric acid and its chromatographic determination"Anal. Lett.. 34. 2771-2784 (2001)

Tarek Samir Awad 等人：“从硼酸中生成乙硼烷及其色谱测定”Lett. 34. 2771-2784 (2001)

Kazuhiko Takeda 他: "Three-year determination of trace metals and lead isotope ratio in rain and snow depositions collected in Higashi-Hiroshima, Japan."Atmospheric Environment. 34. 4525-4535 (2000)

Kazuhiko Takeda 等人：“日本东广岛收集的雨雪沉积物中痕量金属和铅同位素比率的三年测定”。大气环境。34. 4525-4535 (2000)

Kazuhiko Tarada 他: "Three-year determination of trace metals and the lead isotope ratio in rain and snow depositions collected in Higashi-Hiroshima, Japan"Atmospheric. Environment. 34. 4525-4535 (2000)

Kazuhiko Tarada 等人：“日本东广岛收集的雨雪沉积物中痕量金属和铅同位素比率的三年测定”大气环境。