Mechanism of spinal deformity produced by pinealectomy and the role of a circadian dock of the pineal gland on developing the spine in the chick

松果体切除造成脊柱畸形的机制及松果体昼夜节律对雏鸡脊柱发育的作用

• 批准号: 12640647
• 负责人: HATTORI Atsuhiko
• 金额: $ 2.3万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640647/
• 关键词: pineal gland; spine; scoliosis; deformity; chick; bukling; circadian rhythm; biological clock; 側弯; 突発性側弯症; 組織学的手法; メラトニン; 骨代謝; カルシウム; 骨密度; 骨形成マーカー; 骨吸収マーカー

Pinealectomized chickens ax known to develop scoliosis, but the mechanism underlying this phenomenon is unknown. On the other hand, the pineal gland at 1 day-old chick shows a ciicadian rhythm, however, the rhythm dumps out in accordance with aging. Therefore, we investigated the role of a arcadian dock in the pineal gland on the spinal deformity. We examined the rate of spinal deformity in the broilers that are pinealectomized at different ages. The rate of spinal deformity decreased in accordant with removal day of age. These results suggest that the Orcadian dock in the pineal gland is important to develop the spine. We conducted the next study to verify two proposed hypotheses lor the etiology of the spinal deformity: asymmetric growth cfliievertebral bone and the buckling. Tnerefore the influence of body growth on the degree of spinal deformity was investigated Piiiealectomized broilers at 1 day-old were kept under different dietary foods for 2 weeks. Tne mean degree of the spinal deformity in full, 75, and 50% diet was 50,38 and 13°, respectively. These residta show that the induction of spinal deformity in pinealectomized chicks is related to the body growth. Futhermore, the pinealectomized diiciks were sacrificed at 4 days old, and the 76 vertebra was collected and examined ossification in the anterior and posterior parts of the vertebral bone. No difference was found in ossification in the anterior part of vertebral bone, but the area of ossification in the posterior part was significantly larger (P<0.001) in the pinealectomized chicks. At present, scoliotic deformities were thought to occur as a result of causing differences in bone growth between the anterior and posterior elements of the vertebral column, and the buckling phenomenon caused by the marked increase of the body growth.

已知松果体切除的鸡会发生脊柱侧弯，但这种现象背后的机制尚不清楚。1日龄雏鸡的松果体则表现为西卡迪亚节律，但随着年龄的增长，节律逐渐减弱。因此，我们研究了松果体中的Arcadian基座在脊柱畸形中的作用。我们检测了不同年龄切除松果体的肉鸡的脊柱畸形率。脊柱畸形率随摘除年龄的增加而降低。这些结果表明，松果体中的Orcadian Dock对于脊椎的发育是重要的。我们进行了下一项研究，以验证提出的关于脊柱畸形病因的两个假说：不对称生长椎骨和屈曲。在研究生长发育对脊柱畸形程度的影响之前，将1日龄去皮下肉鸡饲养在不同日粮下2周。全日粮、75%日粮和50%日粮的平均脊柱畸形程度分别为50°、38°和13°。结果表明，去松果体雏鸡脊柱畸形的发生与其生长发育有关。并于4日龄处死松果体切除的大鼠，取76个椎体，观察椎骨前、后部骨化情况。去松果体雏鸡椎骨前部骨化无明显差异，但后部骨化面积显著增大(P&lt；0.001)。目前认为脊柱侧弯畸形的发生是由于脊柱前、后部骨生长的差异，以及由于椎体生长的显著增加而导致的屈曲现象。

项目成果

日本比較内分泌学会: "ブルーバックス 生命をあやつるホルモン"講談社. 238 (2003)

日本比较内分泌学会：“Bluebacks：控制生命的激素”讲谈社238（2003）。

Suzuki, M. et al.: "Bisphenol A influences the plasma calcium level and inhibits catatonin secretion in goldfish"Zool. Sci. in press. (2003)

Suzuki, M. 等人：“双酚 A 影响金鱼的血浆钙水平并抑制紧张素分泌”Zool。

Nakayama et al.: "Effects of melatonin and 5-methoxytryptamine under excessive illumination in Cyanobacterium"Proc. Japan Soc. Comp. Endoc. 17. 86 (2002)

Nakayama 等人：“过度光照下褪黑激素和 5-甲氧基色胺对蓝藻的影响”Proc。

Sano et al.: "Effect of melatonin osteoclastic and osteoblastic activities in cultured frontal bones of chicks"Zool. Sci.. 19. 1485 (2002)

Sano 等人：“褪黑激素破骨细胞和成骨细胞活性对培养的雏鸡额骨的影响”Zool。

Hattori: "Melatonin and sleep"Annual Review Neuron. 2002. 7-17 (2002)

服部：“褪黑素与睡眠”神经元年度评论。