Mechanism of spinal deformity produced by pinealectomy and the role of a circadian dock of the pineal gland on developing the spine in the chick

松果体切除造成脊柱畸形的机制及松果体昼夜节律对雏鸡脊柱发育的作用

基本信息

批准号：
12640647
负责人：
HATTORI Atsuhiko
金额：
$ 2.3万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Pinealectomized chickens ax known to develop scoliosis, but the mechanism underlying this phenomenon is unknown. On the other hand, the pineal gland at 1 day-old chick shows a ciicadian rhythm, however, the rhythm dumps out in accordance with aging. Therefore, we investigated the role of a arcadian dock in the pineal gland on the spinal deformity. We examined the rate of spinal deformity in the broilers that are pinealectomized at different ages. The rate of spinal deformity decreased in accordant with removal day of age. These results suggest that the Orcadian dock in the pineal gland is important to develop the spine. We conducted the next study to verify two proposed hypotheses lor the etiology of the spinal deformity: asymmetric growth cfliievertebral bone and the buckling. Tnerefore the influence of body growth on the degree of spinal deformity was investigated Piiiealectomized broilers at 1 day-old were kept under different dietary foods for 2 weeks. Tne mean degree of the spinal deformity in full, 75, and 50% diet was 50,38 and 13°, respectively. These residta show that the induction of spinal deformity in pinealectomized chicks is related to the body growth. Futhermore, the pinealectomized diiciks were sacrificed at 4 days old, and the 76 vertebra was collected and examined ossification in the anterior and posterior parts of the vertebral bone. No difference was found in ossification in the anterior part of vertebral bone, but the area of ossification in the posterior part was significantly larger (P<0.001) in the pinealectomized chicks. At present, scoliotic deformities were thought to occur as a result of causing differences in bone growth between the anterior and posterior elements of the vertebral column, and the buckling phenomenon caused by the marked increase of the body growth.

松果体切除的鸡斧已知会出现脊柱侧弯，但是这种现象的基础机制尚不清楚。另一方面，1天大的鸡肉的松果体显示出一种Ciicadian节奏，但是，节奏会根据衰老倾倒。因此，我们研究了Arcadian码头在松果体中的作用在脊柱畸形中。我们检查了在不同年龄的松果切除术中的脊柱畸形率。根据脊柱畸形的程度，对脊柱畸形的程度降低了1日大的脊柱畸形肉体，将piiieal切除肉肉保持在不同的饮食中，以验证下一项研究，以验证两种提出的假设，使脊髓畸形的病因学：不对称生长cfliievertric cfliieverte cfliieverteplal骨骼和低位。 2周。全，75和50％饮食的脊柱畸形的平均水平分别为50,38和13°。这些残留物表明，松果体切除鸡的脊柱畸形诱导与人体生长有关。 futhermore，在4天大时处死了松果体切除的diicik，并在椎骨的前部和后部收集了76个椎骨并检查了骨化。在椎骨前部的骨化中没有发现差异，但是在松果体切除的小鸡中，后部骨化区域明显更大（p <0.001）。目前，由于椎骨柱的前元和后元素之间的骨骼生长差异以及由于身体生长的明显增加而引起的屈曲现象，因此被认为是由于导致骨骼生长差异而发生的。