Mechanism of spinal deformity produced by pinealectomy and the role of a circadian dock of the pineal gland on developing the spine in the chick

松果体切除造成脊柱畸形的机制及松果体昼夜节律对雏鸡脊柱发育的作用

• 批准号: 12640647
• 负责人: HATTORI Atsuhiko
• 金额: $ 2.3万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640647/
• 关键词: pineal gland; spine; scoliosis; deformity; chick; bukling; circadian rhythm; biological clock; 側弯; 突発性側弯症; 組織学的手法; メラトニン; 骨代謝; カルシウム; 骨密度; 骨形成マーカー; 骨吸収マーカー

Pinealectomized chickens ax known to develop scoliosis, but the mechanism underlying this phenomenon is unknown. On the other hand, the pineal gland at 1 day-old chick shows a ciicadian rhythm, however, the rhythm dumps out in accordance with aging. Therefore, we investigated the role of a arcadian dock in the pineal gland on the spinal deformity. We examined the rate of spinal deformity in the broilers that are pinealectomized at different ages. The rate of spinal deformity decreased in accordant with removal day of age. These results suggest that the Orcadian dock in the pineal gland is important to develop the spine. We conducted the next study to verify two proposed hypotheses lor the etiology of the spinal deformity: asymmetric growth cfliievertebral bone and the buckling. Tnerefore the influence of body growth on the degree of spinal deformity was investigated Piiiealectomized broilers at 1 day-old were kept under different dietary foods for 2 weeks. Tne mean degree of the spinal deformity in full, 75, and 50% diet was 50,38 and 13°, respectively. These residta show that the induction of spinal deformity in pinealectomized chicks is related to the body growth. Futhermore, the pinealectomized diiciks were sacrificed at 4 days old, and the 76 vertebra was collected and examined ossification in the anterior and posterior parts of the vertebral bone. No difference was found in ossification in the anterior part of vertebral bone, but the area of ossification in the posterior part was significantly larger (P<0.001) in the pinealectomized chicks. At present, scoliotic deformities were thought to occur as a result of causing differences in bone growth between the anterior and posterior elements of the vertebral column, and the buckling phenomenon caused by the marked increase of the body growth.

已知切除松果体的鸡会发生脊柱侧凸，但这种现象背后的机制尚不清楚。另一方面，1日龄雏鸡的松果体呈卡氏节律，但随着年龄的增长，这种节律逐渐消失。因此，我们研究了一个阿卡迪亚码头的作用，在松果体对脊柱畸形。我们研究了在不同年龄切除松果体的肉鸡脊柱畸形的发生率。脊柱畸形发生率随取出日龄的增加而降低。这些结果表明，松果体中的Orcadian dock对脊椎的发育是重要的。我们进行了下一个研究来验证两个关于脊柱畸形病因的假设：不对称生长的椎骨和屈曲。在此基础上，研究了机体生长对脊柱畸形程度的影响。全量、75%和50%饮食的脊柱畸形平均程度分别为50 °、38 °和13 °。这些结果表明，雏鸡松果体切除后脊柱畸形的发生与机体的生长发育有关。4日龄时处死切除松果体的大鼠，取76个椎体，检查椎体前、后部骨化情况。去松果体鸡椎体前部骨化无差异，后部骨化面积显著增大（P <0.001）。目前，脊柱侧凸畸形被认为是由于脊柱前后元件之间的骨生长差异以及身体生长显著增加引起的屈曲现象而发生的。

项目成果

日本比較内分泌学会: "ブルーバックス 生命をあやつるホルモン"講談社. 238 (2003)

日本比较内分泌学会：“Bluebacks：控制生命的激素”讲谈社238（2003）。

Suzuki, M. et al.: "Bisphenol A influences the plasma calcium level and inhibits catatonin secretion in goldfish"Zool. Sci. in press. (2003)

Suzuki, M. 等人：“双酚 A 影响金鱼的血浆钙水平并抑制紧张素分泌”Zool。

Nakayama et al.: "Effects of melatonin and 5-methoxytryptamine under excessive illumination in Cyanobacterium"Proc. Japan Soc. Comp. Endoc. 17. 86 (2002)

Nakayama 等人：“过度光照下褪黑激素和 5-甲氧基色胺对蓝藻的影响”Proc。

Sano et al.: "Effect of melatonin osteoclastic and osteoblastic activities in cultured frontal bones of chicks"Zool. Sci.. 19. 1485 (2002)

Sano 等人：“褪黑激素破骨细胞和成骨细胞活性对培养的雏鸡额骨的影响”Zool。

Hattori: "Melatonin and sleep"Annual Review Neuron. 2002. 7-17 (2002)

服部：“褪黑素与睡眠”神经元年度评论。