Analyses of primary structure and production cells of a Bombyx cerebral neuropeptide producing summer morphs in Polygonia c-aureum

经Sephadex G-50凝胶过滤，显示夏季催眠激素(SMPH)活性的家蚕脑神经肽的相对分子质量(Mw)为4500。通过Superose-12柱的凝胶过滤，以上述不同方式部分提取和纯化的SMPH活性多肽的相对分子质量估计为12,000。在这种情况下，SMPH活性多肽可能形成二聚体。我们收集了20000只成年家蚕的脑-食道下神经节复合体。但是，由于不能提供冬春季测定SMPH活性的短日龄何首乌蚕蛹，我们的实验没有进行到现在。在这个项目中，夏季变态的发育被发现是由SMPH介导的。我们成功地建立了一种利用美洲斑潜蝇滞育蛹测定SMPH的方法。现在，我们将对这种生物测定方法进行实验。

Molecular weight (MW) of Bombyx cerebral neuroopeptide showing summer-morph-producing hormone (SMPH) activity was estimated to be 4,500 by gel-filtration using a Sephadex G-50 column. By a gel-filtration using a Superose-12 column, MW of the SMPH-active peptide extracted and purified partially in different ways as above was estimated to be 12,000. It may be that SMPH-active peptide forms a dimmer in this condition. We collected 20,000 of brain-suboesophageal ganglion complexes of adult Bombyx mori. But, our experiments did not go on now, because we could not provide Polygonia short-day pupae for assaying SMPH-actovity in winter and spring.Papilio xuthus has seasonal morphs, spring and summer morphs, whose development is determined by photoperiod and temperature. In this project, the summer-morph development is found to be mediated by the SMPH. We succeeded in establishing an assaying method for SMPH using diapausing pupae of P.xuthus. Now, we are going to try our experiments on this bioassay method.