Purification and Isolation of Polygonia Summer-Morph-Producing Hormone and Bombyx SMPH-Active Substance.

蓼夏变形激素和家蚕 SMPH 活性物质的纯化和分离。

• 批准号: 01540601
• 负责人: ENDO Katsuhiko
• 金额: $ 1.22万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540601/
• 关键词: summer morph; hormone; Polygonia c-aureum; Bombyx mori; purification; 光周性; 人工飼料; カイコガ; イオン交換クロマトグラフィ-

Our present research results consists of 4 parts, the first of which is the characterization of the photoperiodic responses of test animal, Polygoniac-aureum Yamaguchi strain. The second and third parts are the improvement of artificial diet for Un seasonal and mass reproduction of P. c-aureum and the determination of the Bombyx stage at which the brains extracting a summer-morph-producing-hormone-active substance. The 4th part is the methodological confirmation for employing ion-exchange chromatography on a course of Bombyx Summer-Morph-Producing-Hormone (SMPH) active substance purification.The first of third parts of this project were mostly established in this project starting on April, 1989. The 4th part, maybe most important part of this project, was also established mostly, we thought, but remains some problems. in it. That is, we could obtain Bombyx moth brains of more than 20 thousands by October, 1990, when P. c-aureum season starting from May and ending at October was mostly finished. So, we remained an important problem for establishing the ion-exchange chromatography as a purification step of the Bombyx SMPH-active substance

本研究的结果包括四个部分，第一部分是试验动物黄精山口株的光周期反应特性。第二、三部分为金头鱼非季节性大量繁殖人工饲料的改进和脑提取产生夏形激素活性物质的时期的确定。第四部分是采用离子交换色谱法纯化生长激素（SMPH）活性物质的方法学验证，本项目的第一部分和第三部分主要建立在1989年4月开始的本项目中。第四部分，也许是这个项目的最重要的部分，我们认为也基本建立，但仍然存在一些问题。也就是说，到1990年10月，从5月开始到10月结束的金龟子季节已基本结束，我们可以获得2万多只金龟子蛾脑。因此，我们仍然是一个重要的问题，建立离子交换色谱法作为纯化步骤的PGEX SMPH活性物质

项目成果

Fujimoto, Y., Endo, K., Watanabe, M. and Kumagai, K.: "Species-specificity in the action of big and small prothoracico-tropic hormone of four species lepido-pteran insects, Mamestra brassicae, Bombyx mori, Papilio xuthus Polygonia c-aureum." Zool. Sci.8.

Fujimoto, Y.、Endo, K.、Watanabe, M. 和 Kumagai, K.：“四种鳞翅目昆虫（Mamestra brasicae、Bombyx mori、Papilio）的大小前胸激素作用的物种特异性

遠藤克彦: "昆虫の脱皮と変態のタイミングを決めるホルモン." 化学と工業. 43. 36-40 (1990)

Katsuhiko Endo：“决定昆虫蜕皮和变态时间的激素。化学与工业”43. 36-40 (1990)。

Nakaoka, S. and Endo, K.: "Entrainment of the Pro-Thoracico-Tropic Hormone (PTTH) secretion in the swallowtail, Papilio xuthus L. (Lepidoptera : Papilionidae)." Appl. Ent. Zool.25. 95-104 (1990)

Nakaoka, S. 和 Endo, K.：“凤蝶 Papilio xuthus L.（鳞翅目：凤蝶科）中促胸热带激素 (PTTH) 分泌的夹带。”

Endo, K.: "Insect hormones determining the timing of ecdysis and metamorphosis." Kagaku to Kogyo. 43. 36-40 (1990)

Endo, K.：“昆虫激素决定蜕皮和变态的时间。”

Endo, K.: "Hormones determining the seasonal morphs of the butterflies." Insect Physiology ed, Ohnishi et al., Asakura Press. Tokyo. 24-44 (1990)

Endo, K.：“激素决定蝴蝶的季节性变形。”