Analysis of the primary structure of summer-morph-producing hormone and photoperiodic and endocrine regulatory mechanism in the butterfly

蝴蝶夏季形态产生激素的一级结构及光周期和内分泌调节机制分析

• 批准号: 14540628
• 负责人: ENDO Katsuhiko
• 金额: $ 1.98万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540628/
• 关键词: Lycaena phlaeas; pupal body colors; seasonal morphs; Brain of the silkmoth; summer-morph hormone; purify and isolation; amino acid sequencce; primary structure; 蛹の体色; 季節型; カイコガの脳; 精製単離; N-末端のアミノ酸配列; 一次構造; 脳-食道下神経節

a) The small copper butterfly, Lycaena phlaeas daimio, has pupal beige/black polymorphism, development of which is found to be controlled in all apparent association with the development of adult seasonal polymorphism (spring and summer morphs) by photoperiod and temperature given in the larval stages. That is, pupae of beige and black types developed under long-day and short-day conditions tended to develop alto brown-winged and red-winged adults, respectively. In addition, a large proportion of long-day pharate pupae which had been chilled at 4℃ for 5 days developed into pupae whose head-thoracic complexes and abdomens were judged to be black and intermediate types, respectively. They developed into adults with more reddish wings as compared to those developed from the unchilled controlsb) Analysis of the primary structure of Bombyx summer-morph-producing hormone (SMPH) Foamy thousand brain-soboesophageal ganglion complexes were obtaind from the silkmoths, Bombyx mori, by dissection in 0.9% NaCl. A cerebral neuropeptide showing SMPH-activity in the Asian comma butterfly, Polygonia c-aureum. Crude extracts of SMPH-active peptide were purified by a gel-filtration and six steps of reverse-phased HPLC.A peptide fraction showing a suigle peak in absorbance of 220nm was obtained twice and analyzed amino acid sequence of N-terminal of the SMPH-active peptide. As the results, we succeeded to obtain the arrangement of 10 amino acids from the N-terminal of the peptides. However, amino acid sequences of N-terminals obtained by the first and second trials were different partly. We should try to purify the SMPH-active peptide main from Br-SG complexes of the silkmoth and analyze amino acid sequence of the N-terminals

A)小铜蝴蝶Lycaena phlaeas Daimio具有米色/黑色的蛹多态，其发育受幼虫阶段的光周期和温度的控制，与成虫季节多态(春夏季多态)的发展明显相关。也就是说，在长日照和短日照条件下发育的米色和黑色类型的蛹，分别倾向于发育成棕翅和红翅的中高成虫。此外，在4℃冷藏5d的长日照蛹中，有很大一部分发育成头胸复合体和腹部分别为黑色和中间型的蛹。与未冷冻的对照相比，它们发育成更具红色翅膀的成虫b)家蚕夏季形态产生激素(SMPH)泡沫的一级结构分析通过在0.9%的氯化钠中解剖家蚕，获得了数千个脑-食道神经节复合体。一种在亚洲逗号蝴蝶中显示SMPH活性的脑神经肽。SMPH活性多肽的粗提物经凝胶过滤和6步反相高效液相色谱(HPLC)纯化，得到两次在220 nm处有一糖峰的多肽部分，并对其N端氨基酸序列进行了分析。作为结果，我们成功地从多肽的N-端获得了10个氨基酸的排列。然而，第一次试验和第二次试验获得的N-末端的氨基酸序列有部分不同。我们应该尝试从蚕蛾的BR-SG复合体中提纯主要的SMPH活性多肽，并对N-端的氨基酸序列进行分析

项目成果

Tanaka et al.: "Spetial ecology and pattern of setts used in Japanese Badger, Meles meles anakuma"Mammal Study. 27. 15-22 (2002)

Tanaka 等人：“日本獾、Meles meles anakuma 中使用的特殊生态学和固定模式”哺乳动物研究。

Manago J, Tamada K, Okuhisa R, Koda H, Imai H, Yamanaka A, Endo K: "Moth phases in the Councel Center of Forestry of Yamaguchi Prefecture"Biology of Yamaguchi. 29 (in press). (2003)

Manago J、Tamada K、Okuhisa R、Koda H、Imai H、Yamanaka A、Endo K：“山口县林业委员会中心的飞蛾阶段”山口生物学。

Tanaka et al.: "Spacing pattern and sett utilization of the Japanese badger, Meles meles anakuma"Information. 5. 481-490 (2002)

Tanaka 等人：“日本獾 Meles meles anakuma 的间距模式和定位利用”信息。

Muranaka et al.: "Effects of juvenile hormone-I and adult brain-suboesophageal ganglion extracts on ovarian matsuration of reproductive dianuasing femals"Asian Information Life Science. 1(In press). (2003)

Muranaka 等人：“保幼激素-I 和成人脑食管下神经节提取物对生殖障碍女性卵巢成熟的影响”亚洲信息生命科学。

遠藤克彦: "チョウの生物学(第6章 成長と発育(変態)のホルモン調節)"東京大学出版(本田計一, 加藤義臣編). 10 (2003)

远藤克彦：“蝴蝶生物学（第6章：生长和发育的激素调节（变态））”东京大学出版社（本田圭一和加藤佳臣编辑）10（2003）。