Molecular phylogenetic analysis of Wolbachia infection in the Ostrinia furnacalis species group

亚洲玉米螟种群沃尔巴克氏体感染的分子系统发育分析

• 批准号: 12640675
• 负责人: HOSHIZAKI Sugihiko
• 金额: $ 2.3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640675/
• 关键词: Wolbachia; Ostrinia furnacalis; feminization; molecular phylogenettcs; feminization

DNA sequences for two Wolbachia genes (ftsZ and wsp) were determined for more than 20, in total, Wolbachia-infected individuals of four Ostrinia species (O. furnacalis, O. scapulalis, O. orientalis and O. zaguliaevi). These samples shared identical sequences for both genes, suggesting that Wolbachia strains infected in the four moth species are identical or very closely related to each other.DNA sequences for the mitochondrial COII gene were determined for a total of 35 Wolbachia-infected infections in the four host species share the same, single infection event in ancestry. Besides the COII gene was sequenced for a total of more than 200 individuals of seven species including the four Ostrinia species, O. zealis, O. nublialis and O. sp. Phylogenetic analysis of all the COII sequences showed that the Wolbachia-associated COII sequences do not belong to major mitochondrial phylogroups in the seven species. This suggests that the Wolbachia-associated COII lineage has a very old origin in the O. furnacalis species group.Based on the findings, I concluded that the Wolbachia infection has expanded its host range through hybridization of hosts in the O. furnacalis species group. The source species which has supplied the Wolbachia infection to other host species could not be specified from the present findings. The evolutionary origin of Wolbachia infection in the O. furnacalis group is most likely to date back older than speciation events of hosts.

在总共20多个感染沃尔巴克氏体的四种Ostrinia物种（O. Escherichia，O. scapulalis、O. orientalis和O. zaguliaevi）。这些样本共享相同的序列，这表明，沃尔巴克氏体菌株感染的四个蛾物种是相同的或非常密切相关的other.DNA序列的线粒体COII基因被确定为共35沃尔巴克氏体感染的感染在四个宿主物种共享相同的，单一的感染事件的祖先。此外，还测定了Ostrinia 4种、O. zealis、大花野螟O. nublialis和O.所有COII序列的系统发育分析表明，沃尔巴克氏体相关的COII序列不属于主要的线粒体DNA组在7个物种。这表明，沃尔巴克氏体相关的COII谱系有一个非常古老的起源在O。根据这些发现，作者认为Wolbachia感染通过宿主在O.属种组。从目前的研究结果中无法确定将沃尔巴克氏体感染提供给其他宿主物种的来源物种。本文对沃尔巴克氏体感染的进化起源进行了探讨。黑腹叶蝉类群最有可能追溯到比寄主物种形成事件更早的时期。