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Evolutionary biology of Wolbachia, selfish bacterial symbionts in insects : natural history of cytoplasmic incompatibility in a planthopper and sex ratio distortion in moths.

沃尔巴克氏体的进化生物学，昆虫中自私的细菌共生体：飞虱细胞质不相容的自然史和飞蛾性别比例扭曲。

基本信息

批准号：
09839010
负责人：
HOSHIZAKI Sugihiko
金额：
$ 1.86万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Laodelphax striatellus individuals were sampled during 1997-1999 from Honshu in order to examine the spatio-temporal dynamics in the prevalences of Wolbachia infection. PCR assay with ftsZ gene indicated that L. striatellus populations were infected with the B clade of Wolbachia only. Based on the analysis of allozyme polymorphism, it was inferred that the level of long distance migration in L. striatellus is not as high as to prevent genetic differentiation among geographical populations. The long distance migration was discussed in relation to the advance of Wolbachia infection wave in L. striatellus. The relationships between spread of Wolbachia infection and each of mitochondrial DNA variation and reproductive isolation in host insects were discussed under the zoogeographical context.Multiple, maternal lineages expressing thelygeny trait were obtained from each of Ostrinia furnacalis and O. scapulalis populations. Wolbachia infection and feminization of genetic males were strongly associated, and therefore Wolbachia infection was considered to cause feminization in both Ostrinia species. The phylogenetic position of the Wolbachia infected with Ostrinia among major strains of Wolbachia was estimated using DNA sequences of the ftsZ gene. Wolbachia infection showed low prevalences in each of the two Ostrinia species, and high rates of vertical transmission in the laboratory. Five maternal lineages of O. scapulalis showed thelygeny although they were not associated with Wolbachia infection (uSR lineages). Antibiotic treatements and PCR assays indicated that thelygeny in each of the five uSR lineages was not bacterially induced, although was induced by a cytoplasmic/W chromosomal factor(s). The thelygeny in each of the uSR lineages was considered to be underlied with some mechanism(s) other than any of those already known from Lepidoptera.

1997-1999年从本州采集灰飞虱个体，以研究Wolbachia感染流行的时空动态。ftsZ基因的PCR检测结果表明，L. striatellus种群仅感染Wolbachia的B分支。根据等位酶多态性分析，推测长距离迁移的水平可能与长距离迁移的水平有关。striatellus不高，以防止地理种群之间的遗传分化。本文还讨论了长距离迁飞与L.纹状体。从动物地理学角度探讨了Wolbachia感染的传播与宿主昆虫线粒体DNA变异和生殖隔离的关系。scapulalis populations.沃尔巴克氏体感染和遗传雄性的雌性化密切相关，因此沃尔巴克氏体感染被认为是导致这两种鸵鸟属物种雌性化的原因。利用ftsZ基因的DNA序列估计感染Ostrinia的Wolbachia在Wolbachia主要菌株中的系统发育位置。Wolbachia感染在两种Ostrinia物种中均表现出低患病率，并且在实验室中垂直传播率较高。O. scapulalis显示出胚胎发育，尽管它们与Wolbachia感染（uSR谱系）无关。抗生素筛选和PCR分析表明，5个uSR谱系的每一个的胚胎发生不是细菌诱导的，尽管是由细胞质/W染色体因子诱导的。在每个uSR谱系中的雌生殖被认为是以一些机制为基础的，而不是任何已知的鳞翅目机制。