A Novel Method for Estimating Blood Oxygen That Aims In Situ Analysis of Retinal Metabolism - Spectrum colorimetric analysis -

一种旨在原位分析视网膜代谢的血氧估算新方法 - 光谱比色分析 -

基本信息

批准号：
12650415
负责人：
AIZU Yoshihisa
金额：
$ 2.62万
依托单位：
MURORAN INSTITUTE OF TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

The purpose of this study is to develop a method for evaluating blood oxygen, which may be applied in the near future to in situ measurements of retinal metabolism. Some topical results are briefly reviewed below.1. To measure spectral reflectances above a blood vessel and on the surrounding tissue simultaneously, a measuring apparatus was constructed by using a dual-type multi-channel spectrometer and computer.2. A new method was proposed for analysis of spectral reflectances in terms of the dominant wavelength and the excitation purity in the CIEXYZ colorimetric system. According to this method, the analyzing software was developed and successfully used for discriminating between vein and artery.3. By producing spectral reflectance data with various conditions by using the Monte Carlo method, a derivation of oxygen saturation from the dominant wavelength was tried. The result gives rough estimation of the saturation but its quality was not enough.4. To estimate an absolute value of the saturation, a different approach was investigated by using the multiple regression analysis combined with the Monte Carlo simulation. The result improved the estimation.5. The usefulness of this method was verified by experiments with phantom models consisting of the Intralipid solution, pigments and blood. Erroneous estimation was made in case of spectra having noises.6. To apply the proposed method to retinal oxymetry, a method using a reference table that gives directly the oxygen saturation from spectra was newly introduced. The software which follows this method was developed.7. To verify the feasibility of this method, experiments were conducted for samples with known parameters. Measured reflectance spectra were successfully used to estimate the oxygen saturation by using the present method. Measurements with weak light-intensity still suffer from significant errors, but in usual cases results are acceptable.

这项研究的目的是开发一种评估血氧的方法，该方法可以在不久的将来应用于视网膜代谢的原位测量。在下面简要审查了一些局部结果1。为了测量血管上方和周围组织上的光谱反射，使用双型多频道光谱仪和计算机构建了测量设备。2。提出了一种新方法，以分析ciexyz色色系中的主要波长和激发纯度方面的光谱反射率。根据这种方法，开发了分析软件并成功地用于区分静脉和动脉3。通过使用Monte Carlo方法生成具有各种条件的光谱反射率数据，尝试了从显性波长中衍生的氧饱和度。结果对饱和度进行了粗略的估计，但其质量还不够。4。为了估计饱和度的绝对值，通过使用蒙特卡洛模拟的多重回归分析研究了一种不同的方法。结果改善了估计值5。通过使用幻影模型，包括脂质溶液，色素和血液组成的幻象模型的实验来验证该方法的有用性。在光谱有噪声的情况下进行了错误的估计。6。为了将所提出的方法应用于视网膜氧合，新引入了使用参考表直接从光谱中获得氧饱和度的方法。开发了遵循此方法的软件7。为了验证该方法的可行性，对具有已知参数的样品进行了实验。测得的反射光谱成功地用于使用当前方法来估计氧饱和度。光强度弱的测量仍然遭受重大错误，但在常规情况下结果是可以接受的。