In Situ Analysis of Fundus Blood Flow - Real Time Measurements of Blood Vessel and System Development -

眼底血流原位分析 - 血管实时测量和系统开发 -

• 批准号: 10650397
• 负责人: AIZU Yoshihisa
• 金额: $ 2.24万
• 依托单位: Muroran Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650397/
• 关键词: fundus blood flow; retinal blood vessel; retinal reflectance; retinal spectroscopy; blood vessel diameter; blood flow measurements

The purpose of this study is to develop an optical method for real time measurements of retinal vessel diameter and to establish the in situ analyzing system of fundus blood flow by combining with the laser retinal blood flowmeter. Some topical results are reviewed below.1. To measure the retinal spectral reflectance, an experimental apparatus was constructed by using a fundus camera, white light source, optical fiber, and multi-channel spectrometer.2. Analysis of the retinal spectral reflectance measured from human subjects was carried out to determine the optimum wavelength of the light source to be 5 70 nm for high-contrast imaging of vessels. A new method was proposed for optical discrimination of artery and vein using color perception.3. A unit for measuring retinal vessel diameters was developed by using a linear image sensor. In this unit, the scanning time of the sensor was set to be 125 msec and vessels may be imaged over a region of 25-50 pixels on the sensor.4. To determine successfully the vessel diameter from signals of the linear image sensor, a signal-processing software was newly developed by means of the moving average and differentiation of pixel data.5. A calibration test was conducted to the measuring unit. Then, this method was compared with the vessel analysis of video images, and results showed the usefulness of this method.6. The unit for measuring retinal vessel diameters was combined with the laser retinal blood flowmeter, and some software programs were modified to measure simultaneously the blood flow velocity, vessel diameter, and blood flow rate in a real time manner. Finally, the in situ analyzing system of fundus blood flow was established by installing some additional functions.The color perception method for vessel discrimination proposed in this study may potentially be useful for oxygen saturation measurements.

本研究的目的是开发一种实时测量视网膜血管直径的光学方法，并结合激光视网膜血流量计建立眼底血流原位分析系统。一些主题结果回顾如下： 1．为了测量视网膜光谱反射率，利用眼底相机、白光源、光纤和多道光谱仪搭建了实验装置。 2．对人类受试者测量的视网膜光谱反射率进行分析，确定用于血管高对比度成像的最佳光源波长为 5×70 nm。提出了一种基于颜色感知的光学辨别动静脉的新方法。 3．使用线性图像传感器开发了一种测量视网膜血管直径的装置。在此单元中，传感器的扫描时间设置为 125 毫秒，血管可在传感器上 25-50 像素的区域内成像。4。为了成功地从线性图像传感器的信号中确定血管直径，新开发了信号处理软件，利用像素数据的移动平均和微分。 5．对测量单元进行校准测试。然后将该方法与视频图像血管分析进行比较，结果表明该方法的有效性。 6．将视网膜血管直径测量装置与激光视网膜血流量计结合，修改部分软件程序，可同时实时测量血流速度、血管直径和血流速率。最后，通过安装一些附加功能，建立了眼底血流的原位分析系统。本研究提出的血管辨别颜色感知方法可能对血氧饱和度测量有用。

项目成果

Yoshihisa Aizu: "Diffuse reflectance spectra of blood vessels within a skin tissue model"SPIE Proceedings. 3599巻. 58-67 (1999)

Yoshihisa Aizu：“皮肤组织模型内血管的漫反射光谱”SPIE Proceedings 3599. 58-67（1999）。

Yoshihisa Aizu: "Spectrophotometric investigation of skin blood vessels on the basis of color perception"SPIE Proceedings. 3749巻. 735-736 (1999)

Yoshihisa Aizu：“基于颜色感知的皮肤血管的分光光度研究”SPIE Proceedings Vol. 3749. 735-736 (1999)

入江満: "高精度センシングのためのレーザー光制御に関する研究"室蘭工業大学地域共同研究開発センター研究報告. 10号. 31-41 (1999)

Mitsuru Irie：“用于高精度传感的激光光控制研究”室兰工业大学区域协作研究开发中心研究报告第10. 31-41号（1999）。

Yoshihisa Aizu: "Diffuse reflectance spectra of blood vessels within a skin tissue model" SPIE Proceedings. 3599巻(発表予定). (1999)

Yoshihisa Aizu：“皮肤组织模型内血管的漫反射光谱”SPIE 论文集第 3599 卷（待出版）。

Yoshihisa Aizu: "Coherent optical techniques for diagnostics of retinal blood flow"Journal of Biomedical Optics. Vol.4, No.1. 61-75 (1999)

Yoshihisa Aizu：“用于诊断视网膜血流的相干光学技术”生物医学光学杂志。