Hybrid Spectroscopic Topography for Functional Imaging of Oxygenation

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The purpose of this study is to develop hybrid optical topography for bio-medicine, which provides us simultaneous measurements on the flow, concentration and oxygenation of blood together with the depth and thickness of the blood layer in skin tissue.1. Optical absorbance properties of blood were investigated to choose the optimum wavelength of 780 and 830 nm, the latter of which was found to better for sensitivity.2. A new method was proposed for estimation of the blood concentration including optical path length using the time-integrated absorbance of speckle images and their multiple regression analysis based on the modified Lambert-Beer law.3. An apparatus for speckle image acquisition and image processing program were developed and optimized, and used to make experiments using skin tissue phantoms based on the agar solution. The result confirmed the usefulness for dig the relative variation.4. Deconvolution processing was developed on the basis of Gaussian point spread function to sharpen topographic images.5. Analyzing algorithm and program were developed by using our own method with isosbestic wavelengths to estimate the hemoglobin concentration (mol/liter) and oxygenation (%), together with the depth and thickness under certain conditions.6. The proposed method was finally unified with the preceding technique to develop a system for measuring the depth and thickness as well as the blood flow and concentration and oxygen saturation. The system was applied to experiments on skin tissue phantoms and human volunteers to verify the usefulness including resolution.