Development of Growth Acceleration Light Source Equipment for Mushroom )Grifora frondosa, Pholiota nameko) Using a Light Emitting Diode with 1/f Fluctuation

使用1/f波动的发光二极管开发蘑菇（Grifora frondosa、Pholiota nameko）生长加速光源设备

• 批准号: 12650435
• 负责人: HIRAMA Junji
• 金额: $ 2.18万
• 依托单位: Kanazawa Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12650435/
• 关键词: Mushroom; Optimum Growth Condition; Bio Electric Potential; Light Stimuli; Waveletigh Dependence; 1; f Fluctuation; Circadian Rhythm; Morphogenetic Property

In this study, we have been scientifically investigating various optimum growing conditions for mushrooms, such as the culture medium, temperature, humidity, and lighting conditions. We have also precisely measured bio-electric potential signals, which are closely related to mushroom growth activation, in order to study the bio-electric potential reactions to various light stimulus conditions, such as wavelength, luminance and irradiation method, as well as the relationship between light stimuli and morpho genetic properties.We have completed the basic design of a new type of light source equipment to control growth conditions. Our study revealed that fruit body growth and fungal thread in the culture medium are closely related, so we studied the growth stages of the fungal thread and the culture medium states using MRI equipment. The following results were obtained:1. Automated accurate measurement system for measuring bio-electric potential We have designed a device for weather contr … More ol that can artificially set the atmospheric conditions and we have completed an accurate automatic system for measuring bio-electric potentials with high accuracy induced by light stimuli.2 Production of a prototype light source panel We specified the center wavelength as that which is effective for accelerating growth; with this wavelength, we developed a light source using an ultra-luminescence LED panel that can be used at the farm site. Further, we developed effectively control the light source, adding a 1/f fluctuation to the stimulating light. It was revealed that adding fluctuated light to the light stimuli is effective in accelerating mushroom growth.3. Application of the growth control light source panel for use at a farm site The prototype growth chamber has been further improved and a comparative morphological study was done by adding various types of light stimuli. At the same time, sensing of the bio-electric potential was conducted and physical checks on the mushrooms were also performed. The implication is that the growth can be established by observation of the pattern of fluctuations in the bio-electric potential.4. Observation of fungal thread growth inside the culture medium We have used a MRI instrument to non-destructively and non-invasively photograph over a period of time, the process of fungal thread growth from its initial stage in the culture medium through to the development of the primordium. Less

在这项研究中，我们一直在科学地研究各种蘑菇的最佳生长条件，如培养基，温度，湿度和光照条件。我们还精确测量了与蘑菇生长激活密切相关的生物电势信号，以研究不同光刺激条件下的生物电势反应，如波长、亮度和照射方式，以及光刺激与形态遗传特性的关系。我们已经完成了控制生长条件的新型光源设备的基本设计。我们的研究发现，菌丝体在培养基中的生长与菌丝体的生长密切相关，因此我们利用MRI设备对菌丝体的生长阶段和培养基状态进行了研究。得到了以下结果：1。我们设计了一种可以人为设定大气条件的气象控制装置，完成了一套光刺激下高精度生物电势自动精确测量系统原型光源面板的制作我们指定了加速生长有效的中心波长；利用这个波长，我们开发了一种使用超发光LED面板的光源，可以在农场现场使用。此外，我们开发了有效的控制光源，在刺激光上增加1/f的波动。结果表明，在光刺激中加入波动光对蘑菇生长有促进作用。通过添加不同类型的光刺激，进一步改进了生长室的原型，并进行了比较形态学研究。同时，进行了生物电势的传感，并对蘑菇进行了物理检测。这意味着可以通过观察生物电势的波动模式来确定生长。真菌丝在培养基中生长的观察我们利用核磁共振仪在一段时间内对真菌丝在培养基中从最初阶段到原基发育的过程进行了非破坏性、非侵入性的拍摄。少

项目成果

平間淳司: "舞茸へ照射する光質の違いが形態形成へ与える影響-照射方法(連続・1/fゆらぎ)と照射光波長特性の比較-"日本植物工場学会誌. 12・3. 36-40 (2000)

Junji Hirama：“不同光质量照射舞茸对形态发生的影响 - 照射方法（连续/1/f波动）和照射光波长特性的比较 -”日本植物工厂学会杂志 12, 3. 36 -40（ 2000）

Hirama,Junji: "Effect of a Specific Light Quality on the Morphgenetic Properties for Grifora Frondosa. )Part 1) - Comparison of Irradiation Methods )Continuous or 1/f Fluctuating Light Stimuli) and Characteristics of Light Wave Lengh -"Journal of Society

Hirama，Junji：“特定光质量对灰树花形态发生特性的影响。）第 1 部分）- 照射方法的比较）连续或 1/f 波动光刺激）和光波长度的特征 -”Journal of Society

平間淳司: "MRIを用いた舞茸栽培地内部における菌系の発達"日本植物工場学会誌. 12・3. 167-173 (2001)

Junji Hirama：“使用 MRI 开发舞茸蘑菇种植区内的真菌系统”日本植物工厂学会杂志 12・3（2001 年）。

平間淳司: "MRIを用いた舞茸培地内部における菌糸の発達の観測"日本植物工場学会誌. (掲載予定). (2001)

Junji Hirama：“使用 MRI 观察舞茸培养基内的菌丝体发育”，日本植物工厂学会杂志（待出版）。

Hirama,Junji: "Tomografic Imaging of Development of Mycelium Inside of Medium for Grifora Frondosa Using MRI System"Journal of Society of High Technology in Agriculture. 13 (3). 167-173 (2001)

平间淳二：“利用MRI系统对灰树花培养基内菌丝体发育进行断层成像”农业高新技术学会学报。