Development of Optimum Growth Promotion System for Mushroom Controlled by Bio-electric Potential, with a LED as a Light Source

关于蘑菇种植的最适生长条件，已在生长地点进行了充分的初步研究。然而，他们的科学调查需要不断更新。本研究以生物电势为指标，对与激活蘑菇生长密切相关的最适生长条件进行了科学研究，并对光照与形态发生的关系进行了研究。这导致了用于控制农业的创新光源的制造。此外，通过观察伴随着蘑菇生物节律的自主生物电势信号，我们讨论了一种利用生物节律作为生物传感器来控制蘑菇环境光刺激的栽培技术。此外，我们还成功地用核磁共振技术无损地拍摄了真菌菌丝在培养基中的发育过程。真菌菌丝…与子实体地上部生长的关系更多地在地下进行了讨论。具体技术进展如下：(1)研制了精密生物电势自动测量系统。利用研制的设备所获得的知识，研制了一套自动测量系统，用于测量可编程空气成分或光刺激产生的生物电势。(2)利用鱿鱼，在蘑菇果体中测量了超微弱磁场信号。光刺激自感蘑菇超微弱磁场信号的测量，在FT到PT的水平上进行测量，并对其应用进行研究，以分析蘑菇的生理活性条件和健康诊断。(3)光源设备的实际应用，针对最佳栽培条件，将蘑菇本身作为生物传感器。观察特定蘑菇的生物节律，将其作为生物传感器来控制周围蘑菇光刺激的明暗时长。(4)利用磁共振成像技术三维显示真菌菌丝的发育。我们已经成功地生成了从真菌最初种植到子实体孢子扩散的一段时间的真菌断层图像的非破坏性显示。(5)根据实验室生长室内培养蘑菇所获得的结果，制作了蘑菇Farma光源系统的实际使用。然后，为了评价该系统的有效性。(6)探讨了在蘑菇上连续施加直流电场进行生长控制的可能性。研究了生物电势对磁场的响应，以及对培养基中真菌生长的影响的磁共振研究。较少

Sufficient initial studies of the optimum growing conditions for mushroom farming have been made at the growing site. However, their scientific investigations need continuous updating. In this study, using bio-electric potential as an indicator, being closely related to the activation of mushroom growth, scientific studies of the optimum growing conditions have been made, in addition to the studies of the relationship between illumination or morphogenesis. This lead to the fabrication of an innovative light source for control of farming. Furthermore, by observing autonomous bioelectric potential signals, which accompanied the biorhythms of mushrooms, we discussed a farming technique, using the biorhythm as a biosensor to control the environmental light stimuli of the mushrooms. In addition, we succeeded in the non-destructive photography of the development of fungal threads in the medium using MRI. The relationship between the growth of the fruit bodies on the ground and fungal threads … More in the subterranean was discussed. The following is a detail of the technical development :(1)Additional production of a precise auto-measurement system for bioelectric potential.Using the knowledge gained from the fabricated equipment, an automatic system was produced for the measurement of the bioelectric potential, resulting from programmable air components or light stimuli.(2)Using a SQUID, an ultra-faint magnetic field signal was measured in the mushroom fruit body.The measurement of the ultra-faint magnetic field signal of mushroom, self-induced by light stimuli, was measured at the levels of from fT to pT and its application was investigated for the analysis of the physiologically active conditions and health diagnostics of the mushrooms.(3)Practical use of light source equipment, aiming at the optimum farming conditions, using the mushroom itself as a biosensor.Observation of the biorhythm of a specific mushroom, using it as a biosensor to control the duration of light and dark of light stimulation of the surrounding mushrooms.(4)Three-dimensional display of the development of fungal threads using MRIUsing MRI, we have succeeded in generating a non-destructive display of a tomograph of fungus for a period covering the initial planting of the fungus up to the dispersal of spores from the fruiting body.(5)Practical use of light sources at a mushroom farmA light source system was fabricated, based on the results obtained from mushroom farming in a Growth Chamber in the laboratory. Then, in order to evaluate the effectiveness of the system.(6)othersThe possibility of growth control by continuous application of a direct current field to mushrooms was discussed. The bioelectric potential response was investigated with regard to the magnetic field, together with MRI studies of the effects on the fungal growth in the culture medium. Less