The development of an optimal mushroom farming system for the edible mushroom, using the bioelectric potential of the mushrooms as an index, with a LED as a source of illumination

以蘑菇的生物电势为指标，以 LED 作为照明源，开发食用菌的最佳蘑菇养殖系统

• 批准号: 14550429
• 负责人: HIRAMA Junji
• 金额: $ 2.3万
• 依托单位: Kanazawa Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550429/
• 关键词: Mushroom; Optimum Growth Condition; Bio-Electric Potential; Light Stimuli; Wavelength Dependence; Genetic Analysis; Morphgenetic Property; MRI System; 至適生育条件

The scientific study of the optimal growth conditions for mushroom farming, such as culture medium, temperature, humidity, illumination, etc, has been making slow progress. By observing the bioelectric-potential of mushrooms, which is considered to be closely related to the growth, the relationship between light stimulus conditions (wavelength, brightness, and illumination methods) and the morphogenetic properties has been investigated. A new type of light source was developed out of studies of the optimum farming conditions from an engineering standpoint. Photographs were taken non-destructively of the growth of fungal threads by MRI. Furthermore, with regard to the effect of the light quality on the morphogenesis, genetic investigations were made into the expression of DNA by an advanced study of bio-molecules.Here are the outlines of this study :(1)Systematic changes in light stimulus factor :An improvement was made in the accurate auto-measurement system for recording bio-electric … More potentials stimulated by illumination in a purpose-built growth chamber.(2)Measurement of very weak spontaneous magnetic field :The very weak spontaneous magnetic field generated by the mushrooms were measured using a SQUID as a non-destructive and non-invasive measuring technique. The effectiveness of the use of the measured results in the study of ripeness of the fungal threads in the culture medium or growth levels of the pileus was evaluated.(3)Development of a light source at the mushroom farming site :Using the improved purpose-built simple growth chamber, comparative experiments were conducted on morphogenetics with various light stimuli. Bio-electric potential sensing was also undertaken at the same time in order to make health checks on the mushrooms. The effectiveness of the use of mushrooms as a bio-sensor as an environmental monitor was also studied. Currently, a comparison study of morphogenesis under various illumination regimes has been conducted, including continuous illumination, fluctuated illumination, pulsed illumination, etc. However, no clear conclusions have yet been arrived at.(4)Cross sectional photographs taken using MRI :The photographs of the mushroom growth process from fungus through to the formation of primordium have been taken, non-destructively and non-invasively, every two weeks. The results have led to a perspective study of the mushroom ripeness.(5)Genetic analysis :With regard to the influence of the characteristics of illumination on the morphogenesis, the messenger RNA(mRNA) was detected using the DD method and compared for analysis. Less

对蘑菇栽培的最佳生长条件，如培养基、温度、湿度、光照等的科学研究进展缓慢。通过观察蘑菇的生物电势，这被认为是密切相关的生长，光刺激条件（波长，亮度和照明方法）和形态发生特性之间的关系进行了研究。一种新型的光源是从工程角度研究最佳耕作条件而开发出来的。通过MRI非破坏性地拍摄真菌线生长的照片。此外，本文还从光质对形态发生的影响出发，利用生物分子学的先进研究方法，对DNA的表达进行了遗传学研究，主要内容如下：（1）光刺激因子的系统性变化：改进了生物电自动测量系统 ...更多信息 在专门建造的生长室中通过照明激发的电势。（2）极弱自发磁场的测量：利用SQUID作为非破坏性和非侵入性测量技术测量蘑菇产生的极弱自发磁场。在培养基中的真菌线程的成熟度或菌盖的生长水平的研究中使用的测量结果的有效性进行了评价。（3）蘑菇养殖场光源的开发：利用改进的特制简易生长室，进行了各种光刺激的形态发生学比较实验。同时还进行了生物电位传感，以便对蘑菇进行健康检查。还研究了使用蘑菇作为生物传感器作为环境监测器的有效性。目前，形态建成的比较研究在不同的照明制度，包括连续照明，波动照明，脉冲照明等，但尚未得出明确的结论。（4）使用MRI拍摄的横截面照片：每两周非破坏性和非侵入性地拍摄蘑菇从真菌生长到原基形成的过程的照片。这些结果导致了对蘑菇成熟度的前瞻性研究。（5）遗传分析：利用DD法检测了光照特性对形态建成的影响，并进行了比较分析。少

项目成果

松田真人, 平間淳司, 他5名: "マイタケにおける光照射時の自発性極微弱磁場信号計測"日本植物工場学会誌. 16巻1号. 20-23 (2004)

Masato Matsuda、Junji Hirama 等 5 人：“舞茸蘑菇光照射过程中自发超弱磁场信号的测量”，日本植物工厂学会杂志，第 16 卷，第 1. 20-23 期（2004 年）。

坂本香織, 平間淳司, 他6名: "なめこ子実体の光形態形成初期過程における発現遺伝子の解析"農業環境工学関連学会2003年合同大会. 361 (2003)

Kaori Sakamoto、Junji Hirama 等 6 人：“滑子子实体光形态发生早期过程中表达基因的分析”2003 年农业与环境工程协会联席会议 361（2003）。

神野克典, 平間淳司, 他5名: "光刺激に応答した子実体形成初期段階における発現遺伝子の解析"農業環境工学関連4学会2002年合同大会. 161 (2002)

Katsunori Jinno、Junji Hirama 等 5 人：“子实体形成早期响应光刺激的表达基因分析”2002 年四届农业与环境工程学会联席会议 161（2002）。

Hideyuki Yanagibashi, Junji Hirama: "Bio-Electric Potential under Light Stimuli of Grifola frondosa.(Part 3) -Relatinship Between the Environmental Temperature and the Bio-Electric Potential-"Journal of Society of High Technology in Agriculture. 15(2). 94

Hideyuki Yanagibashi、Junji Hirama：“灰树花光刺激下的生物电势。（第三部分）-环境温度与生物电势之间的关系-”农业高新技术学会学报。

Junji Hirama: "Relatinship between the Bio-electric Potential and the Morphogenetic Property of Grifola frondosa when Subjected to Different Light Stimuli"First Japan-Thailand Joint Symposium on Advanced Technologies in Agricultual Production and Post-Pro

平间淳二：“不同光刺激下灰树花生物电势与形态发生特性的关系”首届日泰农业生产及后期先进技术联合研讨会