Functional Analysis of the Promoter for Phytoalexin Synthesis Gene

植物抗毒素合成基因启动子的功能分析

• 批准号: 12660043
• 负责人: YOSHIOKA Hirofumi
• 金额: $ 2.18万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660043/
• 关键词: Phytoalexm; Sesquiterpene cyclase; Rishitm; Potato; Defensegene; Promoter; ファイ卜アレキシン

Exposure of potato tuber tissues to incompatible race of Phytophthora infestans, a pathogen of potato late blight, causes multiple defense responses, including the oxidative burst, accumulation of sesquiterpenoid phytoalexins (lubimin and rishitin). All these reactions are also induced in potato tubers by the treatment with a crude elicitor preparation (hyphal wall component ; HWC) from the mycelium of the fungus. Sesquiterpene cyclase is a key enzyme in isoprenoid pathway for the synthesis of sesquiterpenoid phytoalexin. The expression of the gene in plant-pathogen interactions is not well documented. Here we isolated and characterized CDNA encoding potato sesquiterpene cyclase, vetispiradiene synthase (PVS). PVS was encoded by multiple gene family (PVS1-PVS4). As one molecular approach to elucidation of the mechanisms that regulate the phytoalexin synthesis in potato tubers, we investigated changes with time in the transcript levels of PVS. These members were stimulated by an incompatible and a compatible races of P. infestans. Interestingly, only PVS3 was induced in the potato leaves.We isolated and characterized the PVS3 genes from a potato genomic library.Transient expression of luciferase (Luc) reporter gene under the control of PVS3 promoter showed that high level of Luc activity was induced by the fungal elicitor. These results indicated that the promoter region contains cis-acting element for defense response.

马铃薯晚疫病病原菌致病疫霉（Phytophthora infestans）不亲和小种对马铃薯块茎组织的侵染引起了多种防御反应，包括氧化爆发、倍半萜类植物抗毒素（Lubimin和Rishitin）的积累。所有这些反应也通过用来自真菌菌丝体的粗激发子制剂（菌丝壁组分; HWC）处理在马铃薯块茎中诱导。倍半萜环化酶是合成倍半萜类植物抗毒素的类异戊二烯途径中的关键酶。该基因在植物-病原体相互作用中的表达没有很好的记录。在这里，我们分离和鉴定的cDNA编码马铃薯倍半萜环化酶，香根草合成酶（PVS）。PVS由多基因家族（PVS 1-PVS 4）编码。作为阐明马铃薯块茎中植物抗毒素合成调控机制的一种分子方法，我们研究了PVS转录水平随时间的变化。这些成员受到致病疫霉的不相容和相容小种的刺激。我们从马铃薯基因组文库中分离并鉴定了PVS 3基因，在PVS 3启动子控制下的荧光素酶（Luc）报告基因的瞬时表达表明，真菌诱导子能诱导高水平的Luc活性。这些结果表明，该启动子区含有防御反应的顺式作用元件。

项目成果

Yoshioka Hirofumi: "Plant Diseases and Their Control"China Agricultural Scientech Press. 171 (2001)

吉冈博文：《植物病害及其防治》中国农业科技出版社。

Yoshioka, H., Kuchimura, K. and Doke, N.: "China Agricultural Scientech Press, Beijing, China,"Molecular cloning, expression, and characterization of the cDNA and gene for sesquiterpene cyclase in potato., Shimai, Z., Guanghe, Z. and Huaifang, L.. 36-40 (

Yoshioka, H.、Kuchimura, K. 和 Doke, N.：“中国农业科学出版社，北京，中国，”马铃薯倍半萜环化酶 cDNA 和基因的分子克隆、表达和表征。，Shimai，Z.，

Yoshioka Hirofumi: "Plant Diseases and Their Control"Molecular cloning, expression, and characterization of the cDNA and gene for sesquiterpene cyclase in potato. 171 (2001)

Yoshioka Hirofumi：“植物病害及其防治”马铃薯倍半萜环化酶 cDNA 和基因的分子克隆、表达和表征。

Yoshioka Hirofimi: "Induction of plant gp91 phox homologue by fungal cell wall, arachidonic acid and salicylic acid in potato"Molecular Plant-Microbe Interactions. (印刷中).

Yoshioka Hirofimi：“马铃薯中真菌细胞壁、花生四烯酸和水杨酸诱导植物 gp91 phox 同源物”分子植物-微生物相互作用（正在出版）。