Regulation of sterol absorption in intestine

肠道内甾醇吸收的调节

• 批准号: 12660117
• 负责人: IKEDA Ikuo
• 金额: $ 2.37万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660117/
• 关键词: sitosterol; campesterol; SHRSP rat; plant sterols; cholesterol; absorption; ABCG5; ABCG8; シトスタノール; SHRSP; ブラッシカステロール

Deposition and absorption of various plant sterols, campesterol, brassicasterol, sitosterol, stigmasterol and sitostanol, were investigated in SHRSP rats, which was shown to deposit plant sterols in their body. Deposition and absorption of campesterol were highest among plant sterols examined. Those of sitosterol were also higher. Highly positive correlation was observed between plant sterol content in the liver and the absorption rate. The results suggest that deposition of plant sterols in SHRSP rats depends on their absorption rate. cDNA sequences of ABCG5 and ABCG8 in SHRSP rats were compared with those of WKA, WKY and Wistar rats. No mutation was found in ABCG8. One mutation was found in ABCG5, but this must be confirmed. There was no-difference in expression of ABCG5 and ABCG8 in the intestine among the rat strains examined. Higher the deposition of plant sterols in the body, lower the expression of ABCG5 and ABCG8 in the liver. The results suggest that ABCG5 and ABCG8 might participate in excretion of plant sterols from liver to bile.

研究了菜油甾醇、菜子甾醇、谷甾醇、豆甾醇和谷甾烷醇等植物甾醇在SHRSP大鼠体内的沉积和吸收情况，结果表明，植物甾醇在SHRSP大鼠体内有存款。在所检测的植物甾醇中，菜油甾醇的沉积和吸收最高。谷甾醇的含量也较高。肝脏中植物甾醇含量与吸收率呈高度正相关。结果表明，植物甾醇在SHRSP大鼠体内的沉积取决于其吸收率。将SHRSP大鼠ABCG 5和ABCG 8的cDNA序列与WKA、WKY和Wistar大鼠进行比较。ABCG8基因未发现突变。在ABCG5中发现了一个突变，但这必须得到证实。ABCG 5和ABCG 8在肠道中的表达在所检测的大鼠品系之间没有差异。植物甾醇在体内的沉积越高，ABCG 5和ABCG 8在肝脏中的表达就越低。结果表明，ABCG 5和ABCG 8可能参与了植物甾醇从肝脏向胆汁的排泄。

项目成果

Ikeda et al.: "Mechanisms of phytosterolemia in stroke-prone Spontaneously hypertensive and WKY rats"Metabolism. 50・11. 1361-1368 (2001)

Ikeda 等：“易发生中风的自发性高血压和 WKY 大鼠的植物甾醇血症的机制”代谢 50・11（2001）。

Ikeda et al.: "Mechanisms of phytosterolemia in stroke-prone spontaneously hypertensive and WKY rats"Metabolism. 50. 1361-1368 (2001)

Ikeda 等人：“易发生中风的自发性高血压和 WKY 大鼠的植物甾醇血症机制”代谢。