The enzyme systems of trehalose metabolism on the fruit-body formation of Lentinula edodes
海藻糖代谢酶系统对香菇子实体形成的影响
基本信息
- 批准号:12660156
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Torehalose Is the major soluble carbohydrate in the mycelium, and is supported to function as the main translocate carbohydrate for the fruit-body growth in mushroom fungi. This sugar has been reported to be a stress protectant of cytoplasmic menbrances against dryness, freezing and heat stress. But, the detailed imformation sbout these functions is not well known. Then, we examined the changes of trehalose hydrolyzing enzyme activities (trehalase and trehalose phosphorylase during development of fruit-body of Lentinula edodes. Trehalases from the culture filtrate and mycelial extract of L. edodes were purified and characterized. Molecular masses were estimated to be 158kDa (extracellular enzyme) and 130kDa (intracellular enzyme).The enzymes were composed of two identical subunits and carbohydrate molecules. Therefore, it was shown that the activity of phosphoglucomutase was regulated by trehalose from the study on metabolic regulation of trehalose using Pleurotus ostreatus. On the other hand, the effect of trehalose on the preservation of spawn of mushroom fungi was examined. The survival rates of edible mushrooms such as L. edodes and P. ostreatus increased with culturing in trehalose addition as the growth substrate. The mycelia cultured in trehalose was much more effective than that of the glucose substrate for the mycelial growth and fruit-body formation after freeze treatment and less than 20% (RH) at 4℃.
海藻糖是菌丝体中主要的可溶性碳水化合物,是菌丝体生长的主要转运碳水化合物。据报道,这种糖是细胞质膜抗干燥、冷冻和热应激的应激保护剂。但是,关于这些功能的详细信息并不为人所知。在此基础上,研究了香菇子实体发育过程中海藻糖水解酶(海藻糖酶和海藻糖磷酸化酶)活性的变化。从培养滤液和菌丝提取液中提纯海藻酶并对其进行了表征。分子质量估计为158kDa(胞外酶)和130kDa(胞内酶)。酶由两个相同的亚基和碳水化合物分子组成。因此,从平菇对海藻糖代谢调节的研究中可以看出,海藻糖可以调节磷酸葡萄糖糖化酶的活性。另一方面,考察了海藻糖对蘑菇菌种保存的影响。添加海藻糖作为生长基质,可提高食用菌(L. edodes, P. ostreatus)的成活率。在海藻糖培养基中培养的菌丝在4℃条件下,冷冻处理后菌丝生长和子实体形成的效果明显优于葡萄糖培养基。
项目成果
期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takao TERASHITA: "Effect of trehalose on the spawn storage in some edible mushroom fungi (2) : Effect of preservation In the freezer"Mycoscience. (under submission).
Takao TERASHITA:“海藻糖对某些食用菌菌种储存的影响(2):冰箱中的保存效果”Mycoscience。
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- 影响因子:0
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Takao TERASHITA: "Changes in Trehalose Hydrolyzing Enzyme Activities during Development of Fruit-body and the purification and Some Properties of Mycelial Trehalase from Lentinula edodes 発表予定"Journal of Wood Science.
Takao TERASHITA:“子实体发育过程中海藻糖水解酶活性的变化以及香菇菌丝体海藻糖酶的纯化和一些特性即将发表”《木材科学》杂志。
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Mika MURATA: "Puri fication and characterization of an extracellular acid trehalase from Lentinula edodes"Mycoscience. 42・5. 479-482 (2001)
Mika MURATA:“香菇胞外酸性海藻糖酶的纯化和表征”Mycoscience 42・5(2001)。
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寺下 隆夫: "食用きのこ菌類の菌株保存に対するトレハロースの効果 第1報 冷蔵庫内保存の影響"日本応用きのこ学会誌. 10・1(印刷中). (2002)
Takao Terashita:“海藻糖对食用蘑菇真菌菌株保存的影响第 1 部分:冰箱储存的影响”日本应用蘑菇协会杂志 10/1(印刷中)。
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- 影响因子:0
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Takao TERASHITA: "Effect of trehalose on the spawn storage in edible mushroom fungi (1) : Effect of preservation in the refrigerator"Mushroom Sci. and Biotechnology. 10-1, (in press). (2002)
Takao TERASHITA:“海藻糖对食用菌菌种储存的影响(1):冰箱保存的效果”Mushroom Sci.
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TERASHITA Takao其他文献
TERASHITA Takao的其他文献
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{{ truncateString('TERASHITA Takao', 18)}}的其他基金
Purification and identification of the growth promoting substance from corn fiber and its promotion mechanisms on useful mushroom productions
玉米纤维促生长物质的纯化、鉴定及其对有用菌增产的促进机制
- 批准号:
15580151 - 财政年份:2003
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Structure and function of low-molecular-weight proteins with a cytochrome P450 heme-binding domain derived from the basidiomycete Lentinus edodes
担子菌香菇来源的具有细胞色素 P450 血红素结合域的低分子量蛋白的结构和功能
- 批准号:
10660076 - 财政年份:1998
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Genetic analysis on fruiting-time types of Lentinus edodes
香菇结果期类型的遗传分析
- 批准号:
01560011 - 财政年份:1989
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
On the Suitability of Cultivation of Shiitake(Lentinus edodes {Berk.}Sing) on Synthetic Sawdust Substrates.
论在合成木屑基质上种植香菇(Lentinus edodes {Berk.}Sing)的适宜性。
- 批准号:
63860020 - 财政年份:1988
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research